XB-CLONE-3340528
Clone Name: IMAGE:7797250
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Gene:
ncl
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR885238 [+]
ctggatcctagacacgtgaattaccattctctcccaaacacaacaccggccaccatggtgaaattagctaagggtgcaaagacacaggctaagcccaaga
aggcggcaccaccacccccaaaagatatggaagacagtgaggaagaggaggacatggaagaagaggacagcagtgatgaggagatggaactgcctgtaaa
gaagacaccagccaaaaaggcagccactccagccaaagcaacatctgcaaaggcagccactccagctaaagctacagtcacaccaggaaagaagggagca
actccagcaggagccaagaatggaaagcaggcaaagaaacaagagagtgaagatgaggaagatgactctgatgacgaagctgaagacccgacacctatta
caaacaaaccagttgctaagaaagctgtagcaaaaaaagaggaatctgaggatgatgaagaggatgatgaagatgagtctgaggaggaaaagccagtttc
caagaagccccttgccaagaaccctccagctaaagttacacctgcaaagaaacctactgggaagaaacaggaatctgaagatgaagaagatgagtctgag
gaagaaaagcctgttgccaagaaccctcctgcaaagaaacctgcaggcaaaaaacaagaatctgatgatgaagaatctgaagatgatgaagacgagccta
tggaagttgcaccagctctaaaggggaagaaaactgtccctgccaaagcagatgaggatgacgacgatgatgatgatgatgaggacgacgacgaggatga
cgacgatgatgatgaggaggatgaggatgacga
3' EST: DR885237 [+]tttttttttttttttcagcatttttgtttttgtcaaaagccatagccttctcaatcttcacctctgaaccaagtatctttttcccactcagctttaaagc
ctgctcaacctcttcttcagatgagaaatccacataaccaaacttctttgagtttccaatccttacatcctgcacagttaggttcttcttggaaaagaac
tccctcagagcatctttaagctcatcaaaatcttttgaggcgtttaagttgccaataaaaatggacaatccttctgatgtgtctgtctttgctttttttg
cctcaggaatatttttagtctttggcatttcttttttgcgttttgcagacacttgttgttcttcgtcatcctcatcctcctcatcatcatcgtcgtcatc
ctcgtcgtcgtcctcatcatcatcatcatcgtcgtcatcctcatctgctttggcagggacagttttcttcccctttagagctggtgcaacttccataggc
tcgtcttcatcatcttcagattcttcatcatcagattcttgttttttgcctgcaggtttctttgcaggagggttcttggcaacaggcttttcttcctcag
actcatcttcttcatcttcagattcctgtttcttcccagtaggtttctttgcaggtgtaactttagctggagggttcttggcaaggggcttcttggaaac
tggcttttcctcctcagactcatcttcatcatcctcttcatcatcctcagattcctctttttttgctacagctttcttagcaactggtttgtttgtaata
ggtgtcgggtcttcagcttcgtcatcagagtcatcttcctcatcttcactctcttgtttctttgcctgctttccattc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library constructed at the DOE Joint Genome Institute.Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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