XB-CLONE-3340378
Clone Name: IMAGE:7797126
|
Gene:
ahsg
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: DR885332 [+]
cttctgctctccttgtcactgatttgcattgcctctgctgtacataaaaaaccaacagggaggattgtcgactgtgatagcccagaggccaatgatgcag
ccttaacagctcttcattacatcaatgccaatcaccaccatggatataagtttgttctcaaccaggtggaggacattcatgtactacctgagactgctaa
tggagaagtattctatgtggaacttgaccttttggagtcaaaatgtccttgggttagtcccacacctgctgagaactgcccagtgaggcccagagcagaa
caggctgtggaaggtgattgcaaagttaaactccagaagcagaatggcaattttactgtcattggtgtaagttgcaaatctaaaccagattcagctgaaa
acgtgtttcgggcttgtcccacttgttggttactggcgcctctgaatgacacacaagtggctcatgctgttgatgtagcacttgccgacttcaacaatgg
gaataacactgtgtatttcaagctccatgaaattggccgtgctcagattcagggacctgccaacaataaagtacaggttgagtttatttttgtagcaact
aactgctcaaaggaacaggctttggacagctgtgtggccctgactggagatgatgctcaatatggctcctgtgttggcagtgtaacccagctgccagggg
cacaagatgaagagcttgatgtgcagtgtgctgtctatgaaccacagcaaccccaggttgatccaaagcattccctggtgcctgcgcaaccttcagtagt
tcatgggcattttcaccacaacctacgccattccagccctggtcctcactcatcagaatctaactcagctgaaaagcatctggcattccactctaaatca
gccatca
3' EST: DR885331 [+]attgaacaagctttatggatcagatataaagctttgtaaaatatttgcattaaatgtaattttaagatctgacaagatcaatgtcatgtttatgatttaa
gatcacctatacagtacggttaatacagacatgtattatagatgttgtataatattaaaagaatatctttttgcctggacacagaggcctcacaagtaca
ttagggccggcatgttttccacccagtgatctcttgatggctgatttagagtggaatgccagatgcttttcagctgagttagattctgatgagtgaggac
caaggctggaatggcgtaggttgtggtgaaaatgcccatgaactactgaaggttgcgcaggcaccaggggaatgcttggatcaacctggggttgctgtgg
ttcatagacagcacactgcacatcaagctcttcatcttgtgcccctggcagctgggttacactgccaacacaggagccatattgagcatcatctccagtc
agggccacacagctgtccaaagcctgttcctttgagcagttagttgctacaaaaataaactcaacctgtactttattgttggcaggtccctgaatctgag
cacggccaatttcatggagcttgaaatacacagtgttattcccattgttgaagtcggcaagtgctacatcaacagcatgagccacttgtgtgtcattcag
aggcgccagtaaccaacaagtgggacaagcccgaaacacgttttcagctgaatctggtttagatttgcaacttacaccaatgacagtaaaattgccattc
tgcttctggag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library constructed at the DOE Joint Genome Institute.Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: