XB-CLONE-3339296
Clone Name: IMAGE:7797881
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Gene:
pola2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR886025 [+]
accggccaaagaagacaaggacagggtccattttgttgccgatccctgcacgttgtctgtcaatggtgttatcttaggcataacctctacagacttgctc
ttccatatgggtgcagaggaaataagcagctctgcgggggctccggatcgatttagtcggattcttaaacacattctgacccaaagaagttactatccac
tgtatccacccaatgaagaaataaatgttgactatgaggctttctacagctacgcccctatgcctgtcactccagacatttttatagtcccgtctgagct
tcgctattttatcaaggatgtcacaggctgcatctgcattaaccctgggcggctgacaaagggactggtggggggcacatatgcacgctttctggtcaaa
agtggagccacggggtctgaaggaaagagatctccttgtatttctgctcaggtggtgaggatctaactggggaaacctgagaactgaattaacatctgac
aaatagaactggggtgaagacaatgtacataaagagcccatgtaattaacgagtgctctgaaaggagcctgcagacggctgtatcatcattttgtggatt
tgtatatttttacctgtgtttctttaaactaattcacttaaaaaaaaaaaaaaaaaaaa
3' EST: DR886024 [+]aagtgaattagtttaaagaaacacaggtaaaaatatacaaatccacaaaatgatgatacagccgtctgcaggctcctttcagagcactcgttaattacat
gggctctttatgtacattgtcttcaccccagttctatttgtcagatgttaattcagttctcaggtttccccagttagatcctcaccacctgagcagaaat
acaaggagatctctttccttcagaccccgtggctccacttttgaccagaaagcgtgcatatgtgccccccaccagtccctttgtcagccgcccagggtta
atgcagatgcagcctgtgacatccttgataaaatagcgaagctcagacgggactataaaaatgtctggagtgacaggcataggggcgtagctgtagaaag
cctcatagtcaacatttatttcttcattgggtggatacagtggatagtaacttctttgggtcagaatgtgtttaagaatccgactaaatcgatccggagc
ccccgcagagctgcttatttcctctgcacccatatggaagagcaagtctgtagaggttatgcctaagataacaccattgacagacaacgtgcagggatcg
gcaacaaaatggaccctgtccttgtcttctttggccggt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library constructed at the DOE Joint Genome Institute.Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: