XB-CLONE-3336473
Clone Name: IMAGE:7762841
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR887820 [+]
aaagcaggtctagcccgatcttccaacatgcctttcggtaacacccataataactggaaattgaattattcctctgaggaggaatatcctgacctcacca
agcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggcttcaccttggatgatgtcattca
gactggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggtgttcaaggacctttttgatccc
attattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaagggaggtgatgaccttgacccaaact
atgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagcgtcgtgccattgaaaaacactc
catcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagcagcagcagctgattgatgaccac
ttcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatctggcacaatgaaaacaagacct
tcctggtgtgggtaaatgaagaggaccatctgagagtcatttccatgcagaaaggaggaaacatgaaagaagtcttcagacgcttctgtgagggacttca
gaagattgaagagatctttaaacaagcaggacaccccttcatgtgga
3' EST: DR887819 [+]ttttttttttttttttaaatgtgcaataatataatctttatcagagaattagatttatcttctcttttatgttgattttgaagtttttgttttcttgtgc
tggatcttcctactacatttctgagattatcccacccacatccattacttatgtattcccctgtagatggccattttttgacctctaatgatcttaatgg
ggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatcaatgga
ctgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccactaccatctggacttggtccacttcagagaagcccagcctatcagca
ttggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttggggt
gtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcattccacat
gaaggggtgtcctgcttgtttaaagatctcttcaatcttctgaagtccctcacagaagcgtctgaagacttctttcatgtttcctcctttctgcatggaa
atgactctcagatggtcctcttcatttacccacaccaggaaggtcttgttttcattgtgccagataccacgggcatcaggccagtcacggcccatgccag
aggccagaagcagaggggacactggtttgtcaaacaggaagtggtcatcaatcagctgctgctgctctgcatctgacatatccttg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pSPORT1 vector. This library contains the 1.5-2 kb size fraction (the 0.5-1.5 kb fraction is contained in the NIH_XGC_tropMet3 library). Library constructed at the DOE Joint Genome Institute.Vector Details | Vector Map |
Name: pSPORT1
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: