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XB-CLONE-3335550
Clone Name: IMAGE:7796214 | Gene: h3-3b | Species: Xenopus tropicalis | |
Sequence:
3' EST: DR888413 [+]
Library:
Name: NIH_XGC_tropMet3 | External Dbs: Unigene tropicalis cDNA library, Xenopus IMAGE cDNA Library |
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pSPORT1 vector. This library contains the 0.5-1.5 kb size fraction (the 1.5-2 kb fraction is contained in the NIH_XGC_tropMet2 library).Library constructed at the DOE Joint Genome Institute.
Anatomy: whole organism | Stage: NF stage 62 |
Normalized: No | Subtraction: No |
Vector Details | Vector Map | Name: pSPORT1 Type: phagemid 5' Restriction Site: SalI 3' Restriction Site: NotI |
Usage:
Suppliers:
Search for clone at: Open Biosystems