XB-CLONE-3325118
Sequence:
Full length sequence: BC168077 [+]
gacgggcaccgggtcagtacggcagaacaagacgtggcacctacaggggtttatgtctcactccgacatgagcctggcagcgctggggggcgacgatgca
gccaccctgccccccccttataccccggggccccccaagcccagggaacccctggattgctgggcctgcgccgttctgatcactgcccataacctgctgg
cgggatgtgccaacctgctgatattcgtggtgctgtgcgggggggccctgctgcctgctgccgccatgctgctctacgggttcctgtgtcacccccggtt
ccgccggcccacagagcccctgtgctcccccatcctcacccccgcggcctgcaccgtcctactggtctttggagtcatcttgctgctcccgctgctcatc
ctgggcctggccgcctacgcccgcctcgcccgcaccctgcagctgggctcctgcttcctgccctacagccgcgccgtctaccaaggctccgcccagcgca
agcaagagaccaagggccggcctcaggagagcaaagcctgggtgtaaggggcggagccaccaagtgactgtgcaacagctctacaaatgatgtcatcaat
cctcttatgacctcatcattcctcttatgacctcatccttcctcttatgatgtcactgctttaactcaacgacccacgcccaccatatagcaacggttct
gcatgtctgcgacattgttacggcagtttccacgacctcaagccaatggacggagcagtctaactcactttcaaggggaagttaaactgtctatatatat
atatatatatatatagcatggggggaatttaaaggggatgttcaacttttagtataatgtagagagcgatatttcattggtcttttttttttattatttg
tggtttgaaaatgatttcacttttcgttcagcagcgagctggttgctagggcccaaattaccttagcaaccagggagtggtttgaatgaggggctggaat
atgaataggagaggggttgaatagaaaaatattctgtagagagtgatattctgagacaatttgcaattggtcttcattttttttttattgttggtagctt
tttaattattttgatttttgttgtcagcttggaacttcagcagctgtctggttgctagggtccaattcaccttggtaaccagggagtggtttgtgagagt
gactgatctataaatagtagagggtctggatagacatataactgcattttttacagttttttagttattttgctttttgttcagcagctctatttggtat
tttagcagctatctggttgctagggtcttatttatcctagcaacaaggcagtagttgaaaggagagatgagaatatgaatagtagagggtctggatagac
atataaatgatacaaagtaacaataacaataaaactggagcctcacagagcaaccaaaaacagacttgctcatccatgtaacatgtgtaacaacagtctc
tgtacccctcgccctgtaatctgtttactgtaagcctctgggggggggacatttgatacgaaatataatttttttggtttttcccgtgaaaaaaaaaaaa
aaa
5' EST: DR897033 [+]gacgggcaccgggtcagtacggcagaacaagacgtggcacctacaggggtttatgtctcactccgacatgagcctggcagcgctggggggcgacgatgca
gccaccctgccccccccttataccccggggccccccaagcccagggaacccctggattgctgggcctgcgccgttctgatcactgcccataacctgctgg
cgggatgtgccaacctgctgatnat
3' EST: DR897032 [+]tttttttttttttttcacgcggaaaaaccaaaaaaattatatttcgtatcaaatgtcccccccccagaggcttacagtaaacagattacagggcgagggg
tacagagactgttgttacacatgttacatggatgagcaagtctgtttttggttgctctgtgaggctccagttttattgttattgttactttgtatcattt
atatgtctatccagaccctctactattcatattctcatctctcctttcaactactgccttgttgctaggataaataagaccctagcaaccagatagctgc
taaaataccaaatagagctgctgaacaaaaagcaaaataactaaaaaactgtaaaaaatgcagttatatgtctatccagaccctctactatttatagatc
agtcactctcacaaaccactccctggttaccaaggtgaattggaccctagcaaccagacagctgctgaagttccaagctgacaacaaaaatcaaaataat
taaaaagctaccaacaataaaaaaaaaatgaagaccaattgcaaattgtctcagaatatcactctctacagaatatttttctattcaacccctctcctat
tcatattccagcccctcattcaaaccactccctggttgctaaggtaatttgggccctagcaaccagctcgctgctgaacgaaaagtgaaatcattttcaa
accacaaataataaaaaaaaaagaccaatgaaatatcgctctctacattatactaaaagttgaacatcccctttaaattccccccatgctatatatatat
atatatatata
Library:
Description: Library constructed using poly A RNA and oligo dT primers (Invitrogen SuperScript Plasmid System for cDNA Synthesis and Cloning). After second-strand synthesis, cDNA was size-fractionated on a sepharose 4B-CL 30 cm column and directionally cloned into pCS108 (http://mcb.berkeley.edu/labs/harland/pages/plasmids.html). Library constructed by Russell B. Fletcher in R. Harland's laboratory (University of California, Berkeley, Dept of Molecular and Cell Biology).Vector Details | Vector Map |
Name: pCS108
Type: plasmid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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