XB-CLONE-3312899
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Clone Name: IMAGE:7652973
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Gene:
nsg1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122904 
[+]
GGCTCATTGTGTTGAGAAAAGATGGTGAAACTGGGGAATAATTTCAGTGACAAGGGTGCGAAGCCTCCGATCTGTGAGGATGGATTTGATACAATCCCAT
TGATGACCCCTTTGGATGTCAACCAGCTGCAGTTCCCACCTCCAGACAAGGTGGTTGTGAAAACAAAGACTGAATACGAGCGGGATCATAAGAAAGGCAA
ATTCCGAGCCCCCAAGATTGCTGAATTTACTATCAGTATCAGTGAAGGAGTGTCCGAGAGGTTCAAGGTTACCGTTCTGGTCCTGTTTGCTTTGGCATTT
CTGGCCTGCGTTGTGTTCTTGGTTGTCTACAAGGTGTACAAATACGACCATGCCTGCCCTGAAGGCTTTGTGCTTAAGAACAATCGATGCATTCCGGCAG
CTCTGGATAACTACTACACCGAACAAGATCCCAGCGCTCAAGGAAAGTTTTATACGGTCATCAATCATTACAACCTTGCCAAGCAAACCATCACGCGTTC
CGTATCCCCGTGGATGACCGTCCTGTCAGAAGAGAAGTTGTCTGAGCAAGAGACCGAAGCCTCCCAGAAGTCTGCTTAGCCTGATTGGCGCACGTTTGGC
AATGTTAGGGGACTGAAATGGAACTTTCAAAGTAACTGCTTGCTTGCCGCTGGTAGCCAAGCGGTTAAGGATAATTTAGAGGTAACTTGTATTTATGGTT
AAAAGCGCTTCTGTTAACTGGTATTGCTGTGCACACATAACTGTACAGATCATCCATACAGTAGGTACATCAACGTGAGGGCCCCAACAGTTGTCATTAT
TTGCTAAATAATTTCACACCGATGAAACCACGGGTTTTCTTTATTTTTTCTGGTTACGGATATGATGAAAGAGTGATTGAGGAGATGGTTACAAAGCTAT
AAGAGAGAGTAACGTTGGTTGTGGAACTAAGCCGAGGGCAGTACGACCTGATTTGTCTTGCGAGTAAGCTATGAAAGAAGTAAATATGCCGGAGGATTTC
GTAGGATATATAATGTGCTAAGAAAGGTCCCTAGACTATTTATATTAGATTGACTGCAACGATATTGGGCAGAAGAAGACCATGAAGCCCATCTTTTAAT
ACATATCCTTCAGTGCAGAAAAGGAATACACTTAATGGGACTGTTTTATTAGGATATGGGGGATGCACCCTCATCCTCCATTTAACACCTCCTAAATATG
AGTTTTACACTAAAGGTCCAATCCATAGCCTCCCCTTTGAATGTACAATGATGAACATTTTTCGATTTGATGTTTTTCTAGTTTAGTCGAGTAGCAATAT
ACTGAATCATGTGTGAGTGTTTTTGGCTCATGTTGTAACGATATATACCGTGGAACCCGCTCCTTCTGCATAGTAGGTCTTAATAAATAATGTTTCCATA
GCAGTCATACCTGATTAACGCCCCACGTACCCCAACATTCTGCAATTCAGAACAGTTAATGATTTAGGAAGCCTAGACAGGCCAGTAAACTAATCACTTA
TGAAGTGGTGTAGCACTTTTATTCCCCATATAAAATGACAATTCGGCAAAGAGAGAACAATAAACATTGGGAAACTTCCCCCCCCCTCACCGAGGAGAGT
AATTACACAGTCCAGAGAATACAATCTGAAAGACGCGGTCCAGCAATACATAGTAGGCATTTAAGCCATGGCTGAGGGATTCGAACATGCAGTGAGATGT
AACGGAAGAAGAACTGTCAGTGGGATATTGCTTTCTAGATTAGACCCTTCAGGACAGATATGTATATACGGCTTATATAAGGGGGGGCAGCAAGGAAAAA
CAAAAGATGAACCACAGTGGAAGATCTAGAAGTTCCTTCTCTTGTTTCCAATGCTCAAGAGATGTTTTTGAAAATCAACATCACTACACCTATAGACCCC
ATAAGGCCTTTCCAATGTAAGAATGAACTGTTTTTTCTTTTGCTTTTTTTGGTATCAGACAGGCAATTCCATGATCTATATAGGTTTGATACCATTGAAA
GCAACCCACTTGTTGGTAGGTTTGGACAAAGGGATTAAGGATATTTGAAAATCATTTGAACATTCCTGCTTTAATAGAGCCTGATGGATGCTTTGATTTT
CAGAAGCAAAAAATAGTCCACTGTAAAGTGTTTCCAGCCTCTGTAGCCCAAGGCACAGTAAACCCATTTGTTGAGCTTAACTGGCTTAATCCATTTGCCC
ATTATTATAAAACATAAAAATCTATGTTGAAACACAACCGGAATAAAAGAGAAGATGCTGAATTGACCCTGGACTTAAACATCAGATGTATGTATGTCAT
TGATATAGAGGTTTCCTATATAATAGTATGGTAACAATTTATTTTTACCTGTGGAAGCTCTAATGTTCTCAGTGGCTGAGGACTGGTGTAGGGCTGTGAA
TATTGAAAGGCTCTCATTGGTTACTCAGTGGTTTTGTCAACTACCCACATTTAATAAACATGGACTAGGGCTTTTACCCAACTGAAATGTTCTTAAGAAT
AGAGTTTCCTAACTTTTATAGTAATCACAGAGGTGAATTAACTGTGTATAAATGTGATTTACACTACTACTTTTTATTGTAAGCCCCCCCCTTACAATCT
GACTTTAATGCTGCTTTTCTATCCCGACCATTGGAATAAGTTATCCGTTATAGTGCTAAAAACTGCAGATTTACTGCTAGAAATATCAATCTAAATATTC
CATTTATTCTGGGCCCCTACACGATCTGCTGGGAGTAAAAGCTGACACATTTCTGTACTGTAATGAAGGAGGTAATACAGAATTAGATAGATTGGCATTA
TTGGTGTTTTTTAGGCAGAAAAGGGATAAAAAAAAAAAAAACCTCTCTCTTGTAATATAGTTCAGCTTTTTCATTAAACGCCATTTAACAATACAAATAC
GCAAAGAGTTAGCTGACCGACCACTTAGTCCTGGTTAGGATGTCACTGCACCATAGTAAGGCATTGTAGTTAATGTTGTTGTGCGACCACATCATCCAAT
TAGAAATGCTCGAATTTGCTGCCCCACCATTGACGATTCCGCAAGCATCGCTTCTTACCATGTTAATAGACTCAACACTGTTCAGTCTGTTTGAGAATAT
TGTGTGTTAAGGGGACATCATGCTATCGTTTGCATTATTTTATCCTCATGCTGGTTGAATGTGATTTTTTTTTCCCACAGTGGACATTAAGATTTTTTTT
CTTTATTAATTAATAAAAGTGAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX820540 [+]ttgtgttgagaaaagatggtgaaactggggaataatttcagtgacaagggtgcgaagcctccgatctgtgaggatggatttgatacaatcccattgatga
cccctttggatgtcaaccagctgcagttcccacctccagacaaggtggttgtgaaaacaaagactgaatacgagcgggatcataagaaaggcaaattccg
agcccccaagattgctgaatttactatcagtatcagtgaaggagtgtccgagaggttcaaggttaccgttctggtcctgtttgctttggcatttctggcc
tgcgttgtgttcttggttgtctacaaggtgtacaaatacgaccatgcctgccctgaaggctttgtgcttaagaacaatcgatgcattccggcagctctgg
ataactactacaccgaacaagatcccagcgctcaaggaaagttttatacggtcatcaatcattacaaccttgccaagcaaaccatcacgcgttccgtatc
cccgtggatgaccgtcctgtcagaagagaagttgtctgagcaagagaccgaagcctcccagaagtctgcttagcctgattggcgcacgtttggcaatgtt
aggggactgaaatggaactttcaaagtaactgcttgcttgccgctggtagccaagcggttaaggataatttagaggtaacttgtatttatggttaaaagc
gcttctgttaactggtattgctgtgcacacataactgtacagatcatccatacagtaggtacatcaacgtgagggccccaacagttgtcattatttgcta
aataatttcacaccgatga
3' EST: CX820539 [+]cacttttattaattaataaagaaaaaaaatcttaatgtccactgtgggaaaaaaaaatcacattcaaccagcatgaggataaaataatgcaaacgatagc
atgatgtccccttaacacacaatattctcaaacagactgaacagtgttgagtctattaacatggtaagaagcgatgcttgcggaatcgtcaatggtgggg
cagcaaattcgagcatttctaattggatgatgtggtcgcacaacaacattaactacaatgccttactatggtgcagtgacatcctaaccaggactaagtg
gtcggtcagctaactctttgcgtatttgtattgttaaatggcgtttaatgaaaaagctgaactatattacaagagagaggttttttttttttttatccct
tttctgcctaaaaaacaccaataatgccaatctatctaattctgtattacctccttcattacagtacagaaatgtgtcagcttttactcccagcagatcg
tgtaggggcccagaataaatggaatatttagattgatatttctagcagtaaatctgcagtttttagcactataacggataacttattccaatggtcggga
tagaaaagcagcattaaagtcagattgtaaggggggggcttacaataaaaagtagtagtgtaaatcacatttatacacagttaattcacctctgtgatta
ctataaaagttaggaaactctattcttaagaacatttcagttgggtaaaagccctagtccatgtttattaaatgtgggtagttgacaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
