XB-CLONE-3312869
Clone Name: IMAGE:7652647
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Gene:
cep290
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC166133 [+]
gtgattcgtcatcagcatggcttgctttacaaggaatatatgagtgagcaagaatcttggcaagaggaattaaagaaattaaaagaagaaaatgctaaac
tggagaaccaaaaagaaactgatatggtgcaaatcaaagagtatacgaatttgctagatgctcttaaaatggatcctgatgaaatgaaaaaacaagttgc
tgagaacctgaggaaaatgactgtcctccgggtaaatgagaagttgctcacaaggagatacactactttgcttgaaatggagcaacatctgagaaaagat
aatggaaaactgaaaaatgatatttcagacatggaagctgctgtggcagagcgacttgggtatctgcagagatacaaggaaatagctgctttcaaaatag
catctctacaaagggctttggatgacagtgttccaatatcagagctggaaacagccaataaacaatataatgagctgacttccaagtaccgagacatgct
acaaaaggataatttgctagttcagaggactgctgacatggagcacctagagcatgaaaatgcatctttgaaatcccagattgcattctataataaagaa
ctagaaataacaaaagaaaaatgcaacacattggagcaagccttggaacatattaacaatttggatggtaatcctgcaatggataaagcaaccaaagcca
ttaccaataacgaaatcgtttcaatatctaagaaaataaccatgctggaaatgaaggagttaaatgaaaggcaaagggctgagcattcgcagaagatgta
tgaacatctgagaaccactttaactcaattagaggagcggaattttgaacttgagaccaaatttgcagagcttactaaaatcaacttggaagcacaaaaa
gttgaacaggctttaagagatgatctggccaatagcgtaagtaaagcagtgtctgacgcagataggaaaagaatcatggaactggagaagactgaagcag
aattaaaggctgagatgtccaagttacgtaatgtttcagatatagccaaaatgcaagtttcagcattagcaagtcgtcagcaatcaagagagaaggaagt
ggagtccatgcgaaagcaaattttggattatcaagtatggcctttaataataaaaccagtcatgaaagtttctttttttttttaaagccacaggtctcac
ctctggtgggggaaacagtaaaggggtgataggtaccctttaagggaaaaaacaagatctacaatattacaatatgtatgtacatgtttggttttaataa
tatttgagaaaacgattaaacgattcatcagtccccttgcttaaataatataaataatcagccccttggcaaaccagtgaaatgtcctgcctatcctttt
tgtacatttgtagttgctcatcagtcatagatttcctgtctctttttagctagtgatggatttagtagccaagatatggtagttcttggtagtgatgctg
gctctaataatgtctgacatttgtaagtgacaggactagcattatgttttagactttattcagtatgggctgattccatggggctgtgtccaaacccatc
agtttaaagcatcactcaactttccaacatgtaatcttaaaaaacatctcatcttttaaaattcaccttctccattcccttgcacatcattcaataagtt
atcatgttgtacttttttttatactgtgcactgtctatgtttgtttgtatcattatcatgttatattttattaggtcaatgtacatttttgcatttgctt
gcttatttataatcttaactctcttttgtttgatacaggatcagtctgatgaaaaagcactaattgccaaattacaccaacaaattgtagctcttcagat
cagtgagtctacagcagtgggcaagctggaagctgctacagctaaactacgcaaaatggaggcatacaatttacgtttagaacagaagattgatgataaa
gatcaagcattatattatgcccgtatggaaggtagaaaccgagccaaacacctccgtcaaactgttcactctttgaggcgacagttcagtggtgcccttc
cattggcacagcaggaaaaattttctaaaacaatgatgcagctgcaaaatgacaagttgaaaaccattcatgaaatggagcgtgttcagcaagagcgcag
agaggcagagaaccattcagtggaactagaactaaagctgaaaggtttagaagaactaatcagtaccctgaaagatgccaggggagctcaaaaggttact
gaatggcacagaaagatagaagaattgcgcttgcaggatttaaaactgagtcgtgaactaagtcaaaagaaggaagaaatcaaatatttgaataatatga
tctcagagcaagaacgcacaatcagtatactagaagaagaccttgttcagcaggggaagtttcatgaagaaaggcaaatgtcttgggatcagagagaagt
tgagctagaacgacagcttgacatgtatgagcaccagcaaaatgaagtgctaagtgcagctcaaaagtttgaagaggcaactggagaagttcctgactct
aatttacctttacctgaacaacttgaaattgcactaagaaaaataaaagagcatgtaaaggctgttgtggaaacaaaatctgtatgtagaacattagaag
agaaactaaaggagaaagattcattgttgaggacagctgaaaataatattttgagccgagacagagtgatcaatgaattacggcttcagctgcctgcgac
agcagcaaaagaaacacttctgtctaacctaaatcaaataaaggaagattcagaacagcatcaagctttgaaagttgctcatcaaactgttgccaacatg
caagcaagactgaatcaaaaggaagaaatgctaaagaaatatcaacatcttctggctaaagcaagagaggagcaagaggaagttacaaagaagcatgcag
aagaccttaaagaattgcatcagaaactgaatctctattcagatacttccattaacaattttaaagaagctgctaaagagttcatgaaaaaaccttcact
ccaaattccatcaagtgcaaatttaattcgtttggctgaaatggaacagacagttgctgaacaagaaaactcactggcttccctgtccaaccagctgagg
aaagcaacatcggagctggagaagcagaaacagatcacgatagcaaaagtcaatgaatttgaaaacagcaaaaccaggcttgaagagaagcacattgtgg
agataaaaaaactgaagaacgatgctgaagatttaagagctcgtattgctgaaacagagaaagaaattaattatcttaacactgaactggaggctcaaaa
agaggcgaatacccgagctccaacatcaactatgaagaatcttgtggaacgtctgaaaaaccaagtagcacttaaggaaaaacagcaacaggctttaagt
aaagctcttctgacccttagggcagagatgacctcccaagcagaacagcatattattgctgctgcagcacaaaaaaaaaaaaaaa
5' EST: CX820604 [+]tgattcgtcatcagcatggcttgctttacaaggaatatatgagtgagcaagaatcttggcaagaggaattaaagaaattaaaagaagaaaatgctaaact
ggagaaccaaaaagaaactgatatggtgcaaatcaaagagtatacgaatttgctagatgctcttaaaatggatcctgatgaaatgaaaaaacaagttgct
gagaacctgaggaaaatgactgtcctccgggtaaatgagaagttgctcacaaggagatacactactttgcttgaaatggagcaacatctgagaaaagata
atggaaaactgaaaaatgatatttcagacatggaagctgctgtggcagagcgacttgggtatctgcagagatacaaggaaatagctgctttcaaaatagc
atctctacaaagggctttggatgacagtgttccaatatcagagctggaaacagccaataaacaatataatgagctgacttccaagtaccgagacatgcta
caaaaggataatttgctagttcagaggactgctgacatggagcacctagagcatgaaaatgcatctttgaaatcccagattgcattctataataaagaac
tagaaataacaaaagaaaaatgcaacacattggagcaagccttggaacatattaacaatttggatggtaatcctgcaatggataaagcaaccaaagccat
taccaataacgaaatcgtttcaatatctaagaaaataaccatgctgganatgaaggagttaaatgaaaggcaaagggctgagcattcgcagaagatgtat
gaacatctgagaaccacttnntactcattagagggagcgaattttgaacttgagaccaaaattgcagagcttactaaaatcacttggaagcacaaaag
3' EST: CX820603 [+]tttttttttttttttgtgctgcagcagcaataatatgctgttctgcttgggaggtcatctctgccctaagggtcagaagagctttacttaaagcctgttg
ctgtttttccttaagtgctacttggtttttcagacgttccacaagattcttcatagttgatgttggagctcgggtattcgcctctttttgagcctccagt
tcagtgttaagataattaatttctttctctgtttcagcaatacgagctcttaaatcttcagcatcgttcttcagtttttttatctccacaatgtgcttct
cttcaagcctggttttgctgttttcaaattcattgacttttgctatcgtgatctgtttctgcttctccagctccgatgttgctttcctcagctggttgga
cagggaagccagtgagttttcttgttcagcaactgtctgttccatttcagccaaacgaattaaatttgcacttgatggaatttggagtgaaggttttttc
atgaactctttagcagcttctttaaaattgttaatggaagtatctgaatagagattcagtttctgatgcaattctttaaggtcttctgcatgcttctttg
taacttcctcttgctcctctcttgctttagccagaagatgttgatatttctttagcatttcttccttttgattcagtcttgcttgcatgttggcaacagt
ttgatgagcaactttcaaagcttgatgctgttctgaatcttcctttatttgatttaggttagacagaagtgtttcttttgctgctgtcgcaggcagctga
agccgtaattcattgatcactctgtctcggctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: