XB-CLONE-3312861
Clone Name: IMAGE:7652839
|
Gene:
rtkn
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC135966 [+]
AGGGACTCCCAGTAAGTGTCACACTAGGGAATGGTTAGTGAGTGGCTGCTGTATTGTCATTGGCACAGCTGCTAGTGTCACTCACACTCACACTCACAGT
GGCGCACTCACTCCGGCAGGGAGGCACTCACACAGCTGAGCAGAGGGACATGTCGAGAGGCCTCCGAACTGACACAGACATACAGAGGCGAATAGACCAT
GAGGTGCGGATGAGGGAGGGGGCAGTAAAGTTGCTGGAAGCCTCGACTCAGAGGGAACAGGCTCTAGAGGCTGCAAAGAGCCTCCTGGTCTGTAACAGTC
GTATAATGACTTATATGTCTGAGCTGCAGCACCGCAAGGAGGAGCAAGTTCTGCAGCACAATGCCCGCAGGCCGTCGGAGACAGGCTGCACGGAGGAGAG
ACAGCCCTGCAAGGGGAAACTTTCAGTATCAGACCTGCGCATCCCCCTCATGTGGAAAGACAGTGAATATTTCAAAAACAAAGGAGAGCTGCATCGCTGT
GCCGTGTTCTGTGCCCTTCAGCTGCGCGGGGAGGTCTGGGACACGGAACTGGTCTTTGTGGACAGAACTTCTACTGACATCTGTTTTGAGGATCCCATTG
TATTCTCCGACGTGGATCCTGGCTTTGACCTACACCTGCAGTTATACAGCTGCTCTGTGGATGAAGATTTCTCTCTCTCAGGGACTCCCAGAAGGCTTTC
CACTAAGCTGAGCAGTTCCCTTGGGCGGTCGTCGGGGCGCAGGGTCCGAGCGGCTTTGGATGGTACTCAGGGGAGCCCCTTTAGCAATGGAGGAGGAGGA
GGAGGGGGACCGGTCTTACTCCCAACACCCACTGTGCAAGGTCCCAAATTTCAGCTCCTGGCTCACACTCGCCTGGCGCTCGCTGACGTACAAGATGGCT
TCCGCACCCACGATCTGACCATAAGTGGCACAGAGGAGAGCTCTGGTTGGCTGCCGCTATACGGGAGCGTCTGCTGTCGTCTCACCGCCCAACCAGACTG
TATGAACCAGGAGATCATGTCGGGCTTTCTGCAGATACAGGCCCGGGAGCAGCACGGTTGGGAGCGCGTGTTCTGTGCTCTACACGGATGCCGATTGCTC
TGTTTCCATCAGCGCAGTAAAGCGGATCCAACAGCGGAACCAGAACTGACAATATCTGTTAATAAGGAGACCAGGATCCGGGCTTCAGATCGAGATGGGC
CAGGCCAGGTCCATAGTCTGTCCATTACCAACCGGTACGGCTGCGAGGAGGTGACGCACACGCTCAGGGCCGAGAGTCGGGAGGATCTGCGCCTCTGGAT
GGAGGCTTTCTGGCAGCACTTCTATGACATGAGTCAGTGGAAACAATGCTGCGACGACCTGATGAAGATTGAGACTCCTGCCCCCAAACGCACTCCACTC
GCACTCACAAAGCAGCGCTCTCTGTACCATGACATGGCAGTGTCTGTGCCCCCTGCCCCCTGTGTAGAGGGGCTCCTGCTGCAGGATAATGCGCTGTCCG
ATGAGATCCGCTCCCTCCTCTCCTCTTATTACAGTGAAAGCTATTGAAGGTCTCGGCCCCATCCCTGATCTTTTGTCCCAAAGAATGAAAGAGTTTGAGC
TGCAGAGGGGCCTGGACCCCCCGCCCTGGACCTCCCTGTTCCCCAGCCCAAATTCTGCCCTTCCTTCCTGCTCTTCGTCCTCGGATTGCGACAGCCCCCC
CAGCAGGCCCACCAGGGCTCGGCCAAGGACACACTCTCTGGATGCCAAACTGACTTCTCTAAAGAGCCGGGCCCTGAGGAGCTGTGAGCAGAGCCCCCCC
TGCAGGAGTTCAGAGGCGCCCAGGCCTGCCAGTCCTTCCCCGGGGCCACGGGGCTCTAACAGCAGTGGCAGCAGCACCCCAGAATCAGAGCGGGGGGCTC
AGACCCGTAAGAGTGCCAGGAATCTAAAGAACAGGCTGGACCCTCGCAACTGGCTGCAGAGCCAAGTGTAAACGTTCCGCTCCTCTGGGGTGGAGACTCA
TTCAGACTCCTCCCATTTTCCTGGCAACTCTGAACTGTGCAAGGGCCATTAAGGGTCCAAATTAATGTTCCTTACCCTGACTCGCCGGGTGGGTGCCCTA
AGCTTCTAATAGATTTAATATGTCTTTATATGAGCACAATTAATCCAAGCACAAACCCCTCCCACAACAGGGTGGCAAAGGCCAAAGTGGGACGGCCAGT
CTGCAGTTTGGCTCTCCCATTGCAGGGTGATGGGAGCGAGTTAACGGCCAGCTTCTCGAAATGTAACAAAGAGTTTGCCCCTCCCACTACAGGGTGACAC
AAATGAGGGGGAACTATGGGACATAGGGTTTGCCCCTCCCACTGCAGGGTGACACAAATGAGGGGGAACTATGGGACATAGGGTTTGCCCCTCCCACTAC
AGGGTGACACAAATGAGGGGGAACTATGAGACATAGGGTTTGCCCCTCCCACTGCAGGGTGACACAAATGAGGGGGAACTATGGGACATAGGGTTTGCCC
CTCCCACTGCAGGGTGACACAAATGAGGGGGAACTATGGGACATAGGGTTTGCCCCTCCCACTGCAGGGTGACACAAATGAGGGGGAACTATGGGACATA
GGGTTTGCCCCTCCCACTGCAGAGTGATGCGGATGAGAGGCATGCTTTTGATTGGATACATATTTGAGGCACACCTCCCCCCAGAATGACTGTCCTATAA
AATAAATGATCCGTGCCCCTCCCACTCTAGGGTATGTTGTTTCCATATATGGCAGTGTCCCTCCCACATAAAAAGCATGCCCCTCCCACACTTGACACTC
CCACACATGATAGGAGAATGTCCCTCCCACTATAGTCTTTTGCTTGCCATTTCCGGCCATGTGACCTGATATTAACCCCCTCCTTGCCTGAGAGCCTGTT
GGGCCCCGTGCCAAACCCAATATGGGCCCCTGCCTGGGACAAAGCAATCAGCCTGTACCAAATATCTGCTAACGAGATTCTAATTATAATAAAATATCTA
TGTATCTAAAAAAAAAAAAAAAA
5' EST: CX820612 [+]agggactcccagtaagtgtcacactagggaatggttagtgagtggctgctgtattgtcattggcacagctgctagtgtcactcacactcacactcacagt
ggcgcactcactccggcagggaggcactcacacagctgagcagagggacatgtcgagaggcctccgaactgacacagacatacagaggcgaatagaccat
gaggtgcggatgagggagggggcagtaaagttgctggaagcctcgactcagagggaacaggctctagaggctgcaaagagcctcctggtctgtaacagtc
gtataatgacttatatgtctgagctgcagcaccgcaaggaggagcaagttctgcagcacaatgcccgcaggccgtcggagacaggctgcacggaggagag
acagccctgcaaggggaaactttcagtatcagacctgcgcatccccctcatgtggaaagacagtgaatatttcaaaaacaaaggagagctgcatcgctgt
gccgtgttctgtgcccttcagctgcgcggggaggtctgggacacggaactggtctttgtggacagaacttctactgacatctgttttgaggatcccattg
tattctccgacgtggatcctggctttgacctacacctgcagttatacagctgctctgtggatgaagatttctctctctcagggactcccagaaggctttc
cactaagctgagcagttcccttgggcggtcgtcggggcgcagggtccgagcggctttggatggtactcaggggagcccctttagcaatgga
3' EST: CX820611 [+]tttttttttttttttagatacatagatattttattataattagaatctcgttagcagatatttggtacaggctgattgctttgtcccaggcaggggccca
tattgggtttggcacggggcccaacaggctctcaggcaaggagggggttaatatcaggtcacatggccggaaatggcaagcaaaagactatagtgggagg
gacattctcctatcatgtgtgggagtgtcaagtgtgggaggggcatgctttttatgtgggagggacactgccatatatggaaacaacataccctagagtg
ggaggggcacggatcatttattttataggacagtcattctggggggaggtgtgcctcaaatatgtatccaatcaaaagcatgcctctcatccgcatcact
ctgcagtgggaggggcaaaccctatgtcccatagttccccctcatttgtgtcaccctgcagtgggaggggcaaaccctatgtcccatagttccccctcat
ttgtgtcaccctgcagtgggaggggcaaaccctatgtcccatagttccccctcatttgtgtcaccctgcagtgggaggggcaaaccctatgtctcatagt
tccccctcatttgtgtcaccctgtagtgggaggggcaaaccctatgtcccatagttccccctcatttgtgtcaccctgcagtgggaggggcaaaccctat
gtcccatagttccccctcatttgtgtcaccctgtagtgggaggggcaaactctttgttacatttcgagaagctggccgttaactcgctcccatcaccctg
ca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: