XB-CLONE-3312735
Clone Name: IMAGE:7652962
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Gene:
tacr1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154685 [+]
GAAGGCGGCACTTGTGTGTGAGCTGTAGGACCTGCAGTGGTGCCCAGCACTGGCACATACCCTGGCACTCAGCTCATTCCTGCTGCTCTCACCTCAGCAC
TCACTTTGTTTGGTTTGTTTTAGCTTTAAATATTTCTTTTTGGCTGATGTTTAAGTGTTTGTCGCAGCAGCAACAGCAACAGCAACACAGCAACACAGCA
GATCTTTGCACTGGGAGAAAATTCCTTCAGACCATCCTACTTTCATTTCATGTCCATCTGTCTGAATTACTTAGAGAACAGCACAGGACTGCCCTTGTGT
CATTGTAATGGTTCCATGTAGCTGATACCACTGCTCTGCTCACTGGACTGCCACAGAATTTGGAAGGGTCCCCTAGCCTGTTGCACTGTCTGACAGCCAC
CATGAATATCTCAATGCAGAATTCCTCCATATGTAATTCTACTGGTGGGAATGAAACCTGCATCGTCAACCCCTTTCTTCAGCCTGCCTGGCAAATTGCT
CTCTGGTCCATAGCCTACTCCATCATAGTGATTGTGTCTGTGGTTGGGAACATCATTGTCATGTGGATCATTTTGGCTCACAAGAGAATGAGGACTGTCA
CTAACTACTTCCTGGTGAACTTGGCTTTCGCCGAAGCGTCCATGTCCGCTTTCAATACAGTCATCAACTTCACCTATGCCATCCACAACCATTGGTACTA
TGGACTCATATATTGCAAGTTCCACAACTTCTTTCCCATTTCTGCTGTGTTCTCCAGTATTTACTCTATGACAGCAATAGCCCTGGACAGGTATATGGCC
ATCATCCATCCACTGAAGCCAAGGTTGTCGGCCACAGCCACTAGGATAGTGATTGCTGTCATCTGGGGTTTTGCCTTTGCATTAGCCTTTCCTCAGGGAT
ACTTCGCCACTGTTGAACCTACACCGGATAGAGTTGTTTGCTACATGGAGTGGCCGGAATCTGAGAACAGGAAGTTTGAGAAAGTGTACCAAGTGTTACT
ATTTGGTCTCATCTACTTCCTGCCGCTCCTGGTGATAGGTTGCTCCTACACATTTATTGGAATGACCCTGTGGGCAAGTGAGATTCCCGGTGACTCCTCA
GACCGATATCATGAGCAAGTGGTGGCCAAGCGCAAGGTTGTGAAAATGATGATCGTTGTGGTTTGTACTTTTGCAATTTGTTGGCTGCCATATCACATCT
ACTTCCTGCTTCAGCTTTTCTTACCTGATGAGAGCCAGAAATTCTACCAGCAACTTTATTTGGGGATTATGTGGCTTGGGATGAGCTCTACCATGTACAA
CCCCATCATCTACTGTTGTTTGAATGACAGGTTCCGCATTGGTTTTAAGCACGTTTTCCGCTGGTGCCCATTTATACATGCGGGTGAGTATGAAGGGCTG
GAAATGAAATCCACACGCTATCTTCAAACCCAGAGCAGCATGTACAAAATCAGTCGTATCGAAACCACCGTCTCGTCTGTCCTAAGTGCCAACGACGAAG
ACGCAGAGGAAACCACGAAGAGCAGTAAACGTCTGTCTTTGGATCTTACCTCCAATGGTTCCTCGCGCAGTGTTTGTAAGACTATGTCCGACTCATCCAG
CTTTTACTCCAATAATCTTTCATAAGGGAGACACCATCCAACCATAGCCTTGGGTGTCCCGTCTGCGGTTTCAAAGGCCGCAAGTCCCTGAAGCACGGTG
TGCAGCTCCCATCAGTTTCCACCTTCTTCAGGCCCAAAGTGAACAAGAGCTTGTTTACTATTCCAGAGTCCTTACTCTCACCTTTATAAATTCCATCAGG
GAGACCTTGTTTACAATTCAATGGCATTTAGTCAAGTGGTAGAAGACATTACCACGTATAAAGAATGTAGAATGTTTCCACTAAGTCACATTAGTGGATG
TATATCCATTTACCTCTAAAACAAATTCTCGACTCCTCTATACCCCCATAAACAGCTACTGTGATGTTTACATATTCTCAAGATTTTCAGTTACACTTTC
CTTGTTTGGCCACGTAATGCTGGTCATTGTCAAGGAAAGTATTTAAAGGCCTCTATGCCTTTAGGGGAGGTACATTTTCACTTCTGCAATCAAAGTATTT
TTCTTAAATATCTTCTATAAGATATTTATTCAGTTTTATTTTTTTTTATTATAGTTTTTCAGTACAGATTTCTTGCCTGCCATAAAATATTAACATTTGA
GTTATCTATATAATATAAATGTTTATGTACACTGTATATATATATACATCTGTGTTATTAATTGGAGGGTTGCTTTACAGAGAGTAACTTGACATATATA
TTGTGATGGGATGTATGAAATTCCTCATTTCTGCCTTATTGTTTCACTGCAAACGACCTTCAAGCACAGTTTCATTTTTGATTAAGAATTAAGACTATGT
TATTGACTACTTAACTGCCTTTGCTATGATTGTTTCTGGAGGTTCCTGCTGTACAAAACTACTGTGAAGGTGCCAGTGTTTAGATAAAATATGACAAACA
ATATAAATATATGTTTTCTCCTGTAAAGGGGAAGCAAAGGATTCTTAGAAATGTATTCCTGATGCAGTCGTTATCCTTATTTCAGAGCTGTCCATCTCCA
GGCCTCAGGGGCTTTAATGGGACAGGTATTAGACTAACCACAGTCTTGTTTAAGGTACAGTCATGGCCACGCCAGTAGTTCAGTGAGGCCATGTCCTGAT
TAAAATAGTCTGTGTCCCAAACTGCCCTGGCAAGGCCACTCTTTGGTAGATGGTACTTCTTTTCTTCTCTGTACTTGATTGCTGGGAGGTATGCACTGAC
CCTCCATTATGCACTGGTGAGTCTGAATCACTAGTGACTGTATTTGCACTTAAGCAGGGATATATAGATATACTTTCTGGCCTGTTAATGCCTTTGAAGC
CTATAGTTGGACATCCTCCTCTGCCTTTTGTTGTTTATACCTAAAGTATTATTTTTTTTTTTGATCAGGGAAATGATTTACTGCTTATCCTCAGTTTAAG
AGTATGTGATTCATCATAAACATTTGCAAACCTTCTTCACATAGCGAAGATGTACTTAAAGCTATAACACTTCTATGGAATTATAACAGGGTCTTTACTA
CTATGAATGTGAAAAATAAAAAGTGTGCTTACAATGTAACTATAGTGGGTTTTTTTTTTAATATTCCAGGACTGCCATAGTTATTCCTTATAAAACATTT
AAACAGTGTGTAAACCATAAACACAGTTTCATGTCTGCAATATCAGATCATTTATAAATGGACAAGTATATTTCCTAGAAATATACAGTATATAGTTTGA
ATACCCAACAAAGACATAAGTAGAGTTATACTGTGGTTGTACCACCATCATTTGGGTCCAGATTGACACTTCACCAGCTTTACCCTGTGCCTTTTAGATT
TTAATGGGAAAACTCCACAGCAGGGTAACTTGTAGTATCTTGGAAGTTCAGCATCCATGGAAGAGTGCAGATCTACACATGCACATTCCTTATAGCTCCA
CCTTTGCTATTCCCCCAGCAAGTTGAGAAGGACTTGCTCATTGCCCAGTGCACACCTACAGGGGCACATGGATCATGTGGAGGGTCTCTTAGGTCATTTA
CCAAGACCGAGGGTCTGGCTATGCTTTATACCTTGCATTGGTAAGAAAATCCAGCCCTGTCCTTCCTGCCTTCCACAGGCTCCGTCATGGTCTGTTTCTG
CCTTAGCTCCCTAACTCCTGAATGACATGCAAGTTACGGGACAGGTAGGAAAAGAAGGGGGGAAGCCTATGTGCAACTCATGAATGCAACTCTGCCAAAC
ATAGGCAGCACTGTATTTAAGAGGCAACCTAAGCCAGGTTTTCAGTTCTTAAATTTGACTCTGTTAAGATAAAAGGAACATATTGGCAGGCTGAGACCTT
CTCTGTTATGGAGCTTCTGCGTTGCATCACTTACAAATAGACTTGTCGTAAAAGGTCTCTAGAAGAGTTGGTGCTCCGAAGGCTGCCAGTCTGTGACGTA
GCTCCACATTCATTTACAAACGCACATTTTATAGTTCCTCTGGAAGTAAGAGATATCGAGGGTCAAACTGATGGAATATTTTTTATATTGGGTCAAACAT
TGGCACATTCCACTTGGTGACAACTATTTCCAATATTTTAGAGCACTTGACACTATCAGCTTCCATTATTGGAATGAGGAAACCAAAAACAGCAGGTGAA
TTATTTACATTTTGTAAAAAATGTAAAATTCGGACTATGTATTAACAAGAGGCCTTATTGCTTACAGTGCATGGGTTCATGCAGTCTATGAATGCTTATG
CCACAGTTGTAAGATTTGAATGTAAATAATACAGATTACTAGGGAAAAAAACATTTAGAAAAGCCTTAAATGTTGAATTTCTGGCCATTGTAAATGAGTT
TTACATTGACCAATCACACAGACACAGTTACACAGAGACTCTCTTGATTTGCCATTCCTAGCGCCCACCTTCAACCAGGTCGCACAGATTCCATTCGATG
AAACTCTGTGTTGGCTGTAGAAACAGCTGGACTCGATGTGTATTTGAGCCCTTATTTTGGGATCCTGATTTCTCTATTAAAATCAAACATTCTTACAAAT
GGCAGCCCTGAAATATTCCAACTTAGTTGCGCTCCTCACGTTCTTTTTTGGAGGAACAGCAACATCTAAATATGTTTTTGTATATTTTGTGAATAAATGT
ATCCATTGCTACTGCAAACCAATCAGAAAGCTCCATGATTGAGATCTCCTATTCTGTAGGATACATTCATGATAGAATGGAGTAAACTGATTGGTCCTTT
ATATTTTAGTGTTAATCAATATGTTTGCTACAGTCTAGCACTCTGGCTTTCATGCACCCCGCCATCTTGATAAACACAGTGTATTTGTTTAGCTTTGTAC
TGTGCTCAATGAGCTGTGATGTAGATGTATATCGAGTATGACTGTTTACCTATGTAAATGTTTTCACTCTTAAAACAAAAGCAGTATTTTCACAAATAAA
TGTGTTGTTTCATCAAAAAAAAAAAAAAAA
5' EST: CX820819 [+]aggcggcacttgtgtgtgagctgtaggacctgcagtggtgcccagcactggcacataccctggcactcagctcattcctgctgctctcacctcagcactc
actttgtttggtttgttttagctttaaatatttctttttggctgatgtttaagtgtttgtcgcagcagcaacagcaacagcaacacagcaacacagcaga
tctttgcactgggagaaaattccttcagaccatcctactttcatttcatgtccatctgtctgaattacttagagaacagcacaggactgcccttgtgtca
ttgtaatggttccatgtagctgataccactgctctgctcactggactgccacagaatttggaagggtcccctagcctgttgcactgtctgacagccacca
tgaatatctcaatgcagaattcctccatatgtaattctactggtgggaatgaaacctgcatcgtcaacccctttcttcagcctgcctggcaaattgctct
ctggtccatagcctactccatcatagtgattgtgtctgtggttgggaacatcattgtcatgtggatcattttggctcacaagagaatgaggactgtcact
aactacttcctggtgaacttggctttcgccgaagcgtccatgtccgctttcaatacagtcatcaacttcacctatgccatccacaaccattggtactatg
gactcatatattgcaagttccacaacttctttcccatttctgctgtgttctccagtatttactctatgacagcaatagccctggacaggtatatggccat
catccatccactga
3' EST: CX820818 [+]gaaacaacacatttatttgtgaaaatactgcttttgttttaagagtgaaaacatttacataggtaaacagtcatactcgatatacatctacatcacagct
cattgagcacagtacaaagctaaacaaatacactgtgtttatcaagatggcggggtgcatgaaagccagagtgctagactgtagcaaacatattgattaa
cactaaaatataaaggaccaatcagtttactccattctatcatgaatgtatcctacagaataggagatctcaatcatggagctttctgattggtttgcag
tagcaatggatacatttattcacaaaatatacaaaaacatatttagatgttgctgttcctccaaaaaagaacgtgaggagcgcaactaagttggaatatt
tcagggctgccatttgtaagaatgtttgattttaatagagaaatcaggatcccaaaataagggctcaaatacacatcgagtccagctgtttctacagcca
acacagagtttcatcgaatggaatctgtgcgacctggttgaaggtgggcgctaggaatggcaaatcaagagagtctctgtgtaactgtgtctgtgtgatt
ggtcaatgtaaaactcatttacaatggccagaaattcaacatttaaggcttttctaaatgtttttttccctagtaatctgtattatttacattcaaatct
tacaactgtggcataagcattcatagactgcatgaacccatgcactgtaagcaataaggcctcttgttaatacatagtccgaattttacattttttacaa
aatgtaaataattcacctgctgtttttggtttccctcatc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: