XB-CLONE-3312455
Clone Name: IMAGE:7653171
|
Gene:
app
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC167583 [+]
acgtattccattaaatcgaccgattttattattttttttgttctctctatctaccgtgttcttgtacagatcgttctcctgtgttagtggtgtagaaaat
catgggaattacacattcgtttctttgtgcctgttttatgtgcacactttaggaattgcgaaaaaaaaaaggaaaaaaaatcaaaatcacaaaaaaaaaa
aatctatatataaaaagtataattttttttttctttttcatgtacctttttttttttcttttgagctcaaacctcatttagtactggatgtgcctgcgac
acaatgttttgcagttccttccagcttttaaggggggttgttggacgtctctcttgtaattctaagcactttttttagggaaaggaaaaaagtgcccagc
tggttgaaaattagcagagattctttacaaaaatttaaaaaaaaaaaactttctgctggatgattgtacagaatcattgcttatgaactaatagctttct
acactgtgttacataaataaatttaaaaaaaaaatcggaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
aaaaaaaaaaaaaaaaaaaa
5' EST: CX821306 [+]cggagacggcgagcagctcatttaaaaccagtcgtccatatcacattactgagagtttttttttttataaatacagcaacaatgtctaagggaccagcag
ttggaattgatcttggcaccacttactcatgtgtgggtgtcttccagcatggcaaagtggaaatcattgccaatgaccaaggaaacaggaccacacctag
ttatgtggcttttacagatactgagagattgattggagatgcagctaaaaatcaagttgccatgaaccccacaaacacagtttttgatgccaagagactc
attggccgcagatttgaagatgcagttgttcagtctgacatgaagcactggcctttcaatgttatcagtgacagtggaagaccaaaggtccaggtagaat
ataaagcagagaccaaatctttctatccagaggaaatatcatctatggtcttagttaagatgaaggaaatagctgaagcataccttggcaagactgtaac
aaatgctgtaattactgtcccagcatattttaatgactcccagcgacaggctacaaaggatgctggtactatatctgggctgaatgtactgcgaatcatt
aatgagccaactgctgctgccattgcttatggacttgataagaaggtatagatcactaaaacctacttccatagtaaagggctcaagaatgtgggagggt
ggtttacactgcttagtgttaacctgagcagtaacagcaacaagcctgatagcaatttagttggctgcaggtagtga
3' EST: CX821305 [+]tttttttttttttttgagagccaattcaattcattttattggtctggacccaatttgtttgtacagtacagatactatggttttcctgacttttggagtt
aattcaagtattaatgccccaaaattgacatacagcagcatacatgagaaaatttgaacagctagtacgaaatttgtgtcctaggagttacaagacaaaa
aagtggaatgcagcttttatttagtcaacctcttcaatagtaggaccagaggatgctcctcctgatggggcaccaccagcacctgggaagccaccaggca
taccacctggcatgcccccagcactttgatatagctttgtaataatagggttgcacaccttctccagctctttttgttgatgttcaaactcttctttttc
tgcagtctgatttttgtccagccaagatataacttcattgcatttatccaggattttctgtttgtcttcatcactaatttttcctttaagtttctcatct
tcaacagtagccttcatgttaaatgcgtaagactcaagggaattctttgaagataccttatccctttgcttctcatcttcagctttgtacttctcagctt
cttgaaccatacgctcaatatcttccttgctgagacggcctttgtcattggtaattgtgattttgttctcctttcctgtgctcttgtcaacagcagacac
atttaagataccattggcatcaatgtcaaatgtcacctcgatctgaggaacacctcggggagct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: