XB-CLONE-3312170
Clone Name: IMAGE:7653330
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Gene:
kcnt2l
,
kcnt2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121466 [+]
GATTGAGCCCGAAGAGCATCAGACCTCCTGATCATAGAAAAGTTTCTGCAGGGGAAAGAGAATATCATTACTATGGAGCTGGAGAAAGATGTGCAACCTT
TGCCTCCACGCTATCGATTTCGGGACTTGTTGATGGGTGACTGGCAACAGGATGACAGGGTCCATGTGGAATTTTATGTCAATGAAAACTCGTTTAAGGA
ACGACTAAAGTTATTTTTTATAAAGAATCAGAGATCAAGTTTGAGAGTCAGAGTCTTTAACTGTTCCTTAAAGTTACTGAGTTGCCTCCTCTATATTATA
AGGGTCCTTCTGGATGATCCGAGAGAGAGCAGGGGCGGATGCTGGGAATGTACCAAACAGAATTACTCTGCACAACAGAAATTTGACTGGACCCCAATTA
TTTGGGTAAATAGACCTCTTCCCCTGTGGGGGCTTCAGGTACTCGTGGCGCTAATTAGCTTCTGGGAAACAATGCTTCTCACCTACCTTGGCTACAAGGG
AAACATATGGGAGCAGCTCCTCCGGATCCCCTTCATTCTAGAGATGCTGAACACCCTGCCTTTCTTTGTCACGATTTTCTGGCCTCCGTTGAGGAATGTC
TTCATTCCTGTTTTCCTCAATTGCTGGTTGGCTAAACATGCGCTGGAGAACATGATAAATGACTTGCACCGAGCCATTCAGAGGACCCACTCGGCCATGT
TTAACCAGGTCTTTATTCTGATATGCACCCTCATCTGCCTCATGTTCACCTGCATCTGTGGGATTCAGCATCTGGAGAGAGCGGGCAATAACCTCACCCT
CTTTGACTCCCTGTATTTCTGCATTGTCACTTTCTCCACTGTGGGCTTCGGAGACGTCACCCCAAAGATATGGCCGTCACAGCTCCTGGTTGTCATTATG
ATCTGTGTGGCACTCATTGTGCTGCCCATCCAGGTAACAACAGTCACCCTCTGGTTGTCAGTATTCCAGGCACATCTTCCCCCCTATAGCTTGGTCCTTA
GTGCCTCTTCCAGCAAAGCCAAGTAATACTGGCACCATCACTGGTGTATTAGAATTAGGGTGGCGTAACTAAAGCTAAGTGCAGTATTTGCACTCAGGCC
CCCCAACCCTCCAGACAGGCCCCCTTGTGGACACTAAGGTTGCCACCTGGCTAGTATTTCACCGGCCTAGCTGGTAAAATATCAGCCAAGGGCAGAGCCA
GTATCCACCCCCAGCCAGCCTCTAATGCGCCCCTTTATCCCCCTCCTCATGATGTCATGACTCATGCCTGTGTGATGTCAGGACCCTGCCCCTTGATGTC
ATGACTCCACCCATCAACATCTCAGTCCACCCCCTTATGGACCCACCCCCAGTCCCAGCGTATATCGATATTCTAAACCCTAGTGGACATGCCCCCCTGT
ACCTTCCCCTACCCCCGTGCATGATTGGGTACCGGCATGTTTGCGATTGGTCTCATGGAGGAATGGGCTTGGCGTGGGCCTTCCTGGCAGTTACACCGCT
CTCCCCAGTGTTAGTGTGAATAGTAAGTGTGCCTGTGCTGACCTGCTAGTACACTGTGTTAGATACTGACATCAAGTTGTTATCCTTAAACTACATCTCC
CATGTCTATGAAGATTTTCATTCATCCAGGTCATGGTATATCTAGTATGGGTGAATCTGGAGCAACTGGACTTGTTGGGTGGTCACTGGGGACGTTTCGC
TGCTCGTCCGGGCGGCTTCTTCGGTTCGGCTGGCTGGTGTGGGGGGTCCTCGGCATGTGTGCTCTTCCACTGGTCCAATCACAATGGCACTATGTTGAAA
GAGGTGTAAAAGAGGCCATTCATGTCAAAGTGGAGAAATCATCCCTAAACAGAGGCGGGGGACTTCAACACCATCTGTCTGCTACATACAATGCTGTTCT
AACATCTGTACCCCGGCAGTTTCAGAACTCTTCACACATCCATTCATGCAACTCTAACAAGTAACACCTGTTTGAAGAGTTGCATGATACGTTGGTAATC
AAAGTAACTCCACAGCATTCACACCTCTGTGAGTTTCAGATGTCACCTTTCTGAAGAGTTACACAGTGCCATTGTGATTGGATTGGTGGAGGAGTGTGTG
TGCTGGGGGCTTCCCATGCCGGTCGGTTGGGCTGGGGAGGCTGCTCGGATGGGTGGTGGGGCGTCTTCGGTGATTGCCGGGCAAGTCCAGTTGCTTTGAA
TTTATTTATGCTAGATGTACATCTCCCATCATCCCCTGACAGCCAAGGCCCCTCTCTTCTCTAACGCTCACCCTGATCACATGGAGCCTCTGTGTTTCTG
TATCTGCTGTCACATTTCTGACTTGTTTTTTCTGTTCTTCGCTCTGCCTCGTACATTCCTCGGATAGTGATGGATTTCTCGTCTTCCTCTCTCTCCCTTG
TGTCTGTATGACGAGCTGTATGCGGGACACACGCTGGGCTTCCCGGCGCCATCTCATCCGCCGCCGATGACACTTGTGATTAGATGAGCTGACAGATAAA
AGAACAGGGGAGAGAGAAAGGAAGACAACAAAACCCTCACTGTCACCTACTCCCCTGACTAAGGGCCCGTGTAACAAGTCATGTTTAAAGGTTAAAAACA
ATGTATCGTTTAACCTGCCTGAAAGCGCCTCTGCCTCTCCTGCTTGGCTGAATGTTTAAAGAGATACCATTGGCTCATCATTCCTACATTTTTTTTGTAT
TATTCCATTAGGGGTTGTTCACCTTTATGATGATATTCTGAGAATTTGCAATTGGTCTTCATTTTTTATTATTTGTGATTTTCAAATGATTTAGCTTTCT
ATGTAGCAGCTCTCCAATTTGGAATGTAAGCAGCTATCTGGTTGCTGGGGTCCAAACTACCCTAACAACCAGGCTTTGATTTGAATGAGAGACGGGAATA
TGAATAGGAGAGGGACTGAATAGAAAGATAAGTAATAAAGAGTAGCGGCAACAATAAAATTGTAAGCTCACGCAACAATAGTCAGTGACCCGCAATTGAA
AGCTGGAAAAAAAAAAAAAAA
5' EST: CX821776 [+]ttgagcccgaagagcatcagacctcctgatcatagaaaagtttctgcaggggaaagagaatatcattactatggagctggagaaagatgtgcaacctttg
cctccacgctatcgatttcgggacttgttgatgggtgactggcaacaggatgacagggtccatgtggaattttatgtcaatgaaaactcgtttaaggaac
gactaaagttattttttataaagaatcagagatcaagtttgagagtcagagtctttaactgttccttaaagttactgagttgcctcctctatattataag
ggtccttctggatgatccgagagagagcaggggcggatgctgggaatgtaccaaacagaattactctgcacaacagaaatttgactggaccccaattatt
tgggtaaatagacctcttcccctgtgggggcttcaggtactcgtggcgctaattagcttctgggaaacaatgcttctcacctaccttggctacaagggaa
acatatgggagcagctcctccggatccccttcattctagagatgctgaacaccctgcctttctttgtcacgattttctggcctccgttgaggaatgtctt
cattcctgttttcctcaattgctggttggctaaacatgcgctggagaacatgataaatgacttgcaccgagccattcagaggacccactcggccatgttt
aaccaggtctttattctgatatgcaccctcatctgcctcatgttcacctgcatctgtgggattcagcatctggagagagcgggcaataacctcaccctct
ttgactccctgtatttctgcattgtcactttctccactgtgggc
3' EST: CX821775 [+]tttttttttttttttccagctttcaattgcgggtcactgactattgttgcgtgagcttacaattttattgttgccgctactctttattacttatctttct
attcagtccctctcctattcatattcccgtctctcattcaaatcaaagcctggttgttagggtagtttggaccccagcaaccagatagctgcttacattc
caaattggagagctgctacatagaaagctaaatcatttgaaaatcacaaataataaaaaatgaagaccaattgcaaattctcagaatatcatcataaagg
tgaacaacccctaatggaataatacaaaaaaaatgtaggaatgatgagccaatggtatctctttaaacattcagccaagcaggagaggcagaggcgcttt
caggcaggttaaacgatacattgtttttaacctttaaacatgacttgttacacgggcccttagtcaggggagtaggtgacagtgagggttttgttgtctt
cctttctctctcccctgttcttttatctgtcagctcatctaatcacaagtgtcatcggcggcggatgagatggcgccgggaagcccagcgtgtgtcccgc
atacagctcgtcatacagacacaagggagagagaggaagacgagaaatccatcactatccgaggaatgtacgaggcagagcgaagaacagaaaaaacaag
tcagaaatgtgacagcagatacagaaacacagaggctccatgtgatcagggtgagcg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: