XB-CLONE-3311364
Clone Name: IMAGE:7653832
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Gene:
nr1d1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC135990 [+]
GGAGGAGAGACAGGACGTGTAGTAGTGAGCTCCCAATACTCCGGCTACAGCTGCAGCACATTGCCCCGGGAGCGCTAACACTGTGCAACCTCCGTGCTAA
AGGAACATTCCTCTTAAATTCTATCGCAGCCCCATAGGCACCTACAGGCTTGGCTGCTAGTTCTATTGGAGCATAGACTTCAGCTTATATCCAGAGAAAT
ACGGGATTTGCTGTTCCACCAATGCTGCAGAGGCTGATGCCCTTAACCCCCCAAAACCCTTGTGCAAGGATTGCTTGTTGGGGGTATCTGCTGCCAGGAG
CTTTGTAACCGACCCCGACCCAACTTGCTGTGTATGGTTCTGGGACCCCCAAACATGACTACGCTGGATTGTGCTAATAACACCGGAGGTGTAATAAGCT
ACGTGGGCTCCAGTGGCTCATCGCCCAATCGGACCAGCCCTGTCTCCATGTACAGCGACTGCTCTAGTGGGAGCCCCCAGAGTGGTGTCAGCCACTATGC
CAACTACTTGCCTCCATCTCCCTCCCACTCCTACAGTGCCAGCTCCTCTGGATCTGGGGGAGAAGCCTCGCCACGCTCGTCCTACGGTCTTGCAGCTTCC
CCGGGGGGGCTTCATGTTGCACTGGATGATGGAAGCAGGGTGTCTCCTAGCAAAACCTCCAGCAGCATTACCAAGCTGAATGGTATGGTCCTTCTGTGCA
AAGTCTGTGGAGATGTAGCTTCTGGATTTCATTATGGCGTCCATGCTTGTGAAGGTTGCAAGGGCTTTTTCCGACGCAGCATCCAGCAGAACATCCAGTA
CAAGAAATGCCTGAAGAATGAGACTTGCTCTATAGTGAGGATCAATAGGAACCGCTGCCAGCAGTGCCGCTTCCGCAAGTGTCTCTCCGTGGGCATGTCT
CGAGATGCTGTGAGGTTTGGTCGTATCCCAAAGAGAGAGAAGCAGCGGATGCTGGCAGAGATGCACAGTGCTATGAACCACATTGCCAGCGGCCATTTTG
GAAGGCGAAGTCCAATTCCACTTCAACCCCATCAACAGCAGCAGCAGCAGCTCCATCCCTCCTCCTCTCCCCTCATGGGCACAGCGCCATGCCACTCGCC
CACGTCTCTGCCCGATGCCTACTCACAGTACCCGCAGCAGCTGACCCCACCTAGATCTCCCAGTCCGGAGCACATGGATGATGTTATCAGTCAGGTGTCC
ATGGCGCACAGGCAGATCTTTGTCTATGCCAATGACAAAGTGAACAAAAAGACTTCCTGGGACCATCCTCAACCGTGCTGGTCACATGACACTGAGGATT
CCCATGGCAACCATAACATTCGGCTGGCTTGCCCCATGAATGCTCTACATCGGGGAGGTCCCGTCCGCTCTGTGCAAGAGGTGTGGGAAGACTTCTCACT
CAGTTTCACCCCAGCTGTGCGAGAGGTAGTGGAATTTGCCCGTCACATTCCAGGGTTCAAAGACCTAACTCAGAACGACCAGGTCACCCTGCTCAAGGCT
GGCACATTTGAGGTACTGATGGTGCGCTTTGCCTCCTTGTTTGATGCTCGGGAACATTCCTTGAGGTTCCTCAGCGGCGCCACTTACTCCCTTCCTGAAC
TGCATGCTATGGGAATGGGTGAACTTCTCAGCTCCATGTTCGACTTCAGTGAAAAGCTGTCATCTCTCAGTCTGAGCCAGGAGGAGCTGGGCATCTTTAC
AGCACTTGTACTGGTGTCTGCAGATCGCTCTGGGATGGAGAATTCCTCCTTAGTAGAGCAGCTTCAAGAGACTCTCATACGTGCCCTTCGTTCCCTCATC
CTGAAGAACAGTCCAAACGATACCTCCCGCTTCACCAAGCTGCTCCTGCGCCTCCCGGACCTGCGCACCCTGAACAACATGCACTCCGAGAAACTGCTCT
CCTTTCGTGTCGATGCCCACTGATGGCACCATGGAGCCTAAGCGTTGGTTGGGCAGAGCACTGGCACTTTAAACTGAACCTAGAAAGCCGAGTGGCTATA
CAAGCTGTGCCTATTTGGAGATATGGCCGATCACGGGCAGGACGGACGGAGGTGGAATCATAGTGATAAGGTGGCAGGGGGATCACAGGAAGCTGCTAAA
ACATGGATGAACCACATGACGATGATGAAGTATGACATCAGCTTTGTGCCGTTGACCTCATAGACAGTAATTCAATGGTATAGACTCTTCCAAGAGATGG
GAAAGGCTGAATCATGGCTCTCTTGTTTGGGAACATTTTCAGAGGGATGTACATAGACCTGTATATATATAGATATATAAATATCACATAAATATGTACA
AATGACCTGTAAATAAGAACCTGGTGGATATAAATATGAATATTTTATTCAATACTTTAAAGTCTCTGTCTCACTACCCTTCCCGCCCAATGGCCGTTGG
GCTCACAGTGCTACGCTTTGCATGTTCTCCTTGTATTGTCCTACAAAGTAGCGTAGGCTGTTCTGACTTCAGAATGTTCTCAATACATATATAAGGGATG
AAAGCCATGTGCCCCCCATCTTGGATTCTACAACGCATCACGGTATATCCGTTTGGGTTTCTGAAACAGGACAGACTTATCGACGTGACTATACAGGGTT
ATAGGGGCACCGTGCTGTGAAGATCTCATTTCTTTGTGTGTGTGTGTGTAAATATATGTTTTATTTATTTTTTTTGTAACTATTTATAAATCAATACTTC
AGCTACCAATAAATAAATGTTTTACCAAAAAAAAAAAAAAA
5' EST: CX823020 [+]gaggagagacaggacgtgtagtagtgagctcccaatactccggctacagctgcagcacattgccccgggagcgctaacactgtgcaacctccgtgctaaa
ggaacattcctcttaaattctatcgcagccccataggcacctacaggcttggctgctagttctattggagcatagacttcagcttatatccagagaaata
cgggatttgctgttccaccaatgctgcagaggctgatgcccttaaccccccaaaacccttgtgcaaggattgcttgttgggggtatctgctgccaggagc
tttgtaaccgaccccgacccaacttgctgtgtatggttctgggacccccaaacatgactacgctggattgtgctaataacaccggaggtgtaataagcta
cgtgggctccagtggctcatcgcccaatcggaccagccctgtctccatgtacagcgactgctctagtgggagcccccagagtggtgtcagccactatgcc
aactacttgcctccatctccctcccactcctacagtgccagctcctctggatctgggggagaagcctcgccacgctcgtcctacggtcttgcagcttccc
cgggggggcttcatgttgcactggatgatggaagcagggtgtctcctagcaaaacctccagcagcattaccaagctgaatggtatggtccttctgtgcaa
agtctgtggagatgtagcttctggatttcattatggcgtccatgcttgtgaaggttgcaagggctttttccgacgcagcatccagcagaacatccagtac
aagaaatgcctgaagaatgagacttgctctatagtgaggatcaata
3' EST: CX823019 [+]tttttttttttttttggtaaaacatttatttattggtagctgaagtattgatttataaatagttacaaaaaaaataaataaaacatatatttacacacac
acacacaaagaaatgagatcttcacagcacggtgcccctataaccctgtatagtcacgtcgataagtctgtcctgtttcagaaacccaaacggatatacc
gtgatgcgttgtagaatccaagatggggggcacatggctttcatcccttatatatgtattgagaacattctgaagtcagaacagcctacgctactttgta
ggacaatacaaggagaacatgcaaagcgtagcactgtgagcccaacggccattgggcgggaagggtagtgagacagagactttaaagtattgaataaaat
attcatatttatatccaccaggttcttatttacaggtcatttgtacatatttatgtgatatttatatatctatatatatacaggtctatgtacatccctc
tgaaaatgttcccaaacaagagagccatgattcagcctttcccatctcttggaagagtctataccattgaattactgtctatgaggtcaacggcacaaag
ctgatgtcatacttcatcatcgtcatgtggttcatccatgttttagcagcttcctgtgatccccctgccaccttatcactatgattccacctccgtccgt
cctgcccgtgatcggccatatctccaaataggcacagcttgtatagccactcggctttctaggttcagtttaaagtg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: