XB-CLONE-3309786
Clone Name: IMAGE:7655519
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Gene:
prickle2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC153335 [+]
AACAAAGCTGTGGTTCTGAAAGAGACCCTTTAAACAGCACAGCCTTCTCCAAAGAGGGCAGGAATCACTCTCTACTGCTGATGCTTCTCAACAGAACACA
TTCGCTTCCAGCCAGCACAATGCTCGGTGTGCGGGGCTGCTTGGAGAGGCTTTGACTAAAGCCGGCTGCTCATTTCAACTGTCCCTGGGTCTGTCATGCT
GGGAGAGCAGAGGTAACCCCCTTGTGTCCCCTGTGCACAGCCGAGATGTTTGTTGTGCCGGCAACATGGAAAAGAGAATCAAACTGTAAAAACTAAGGAG
CTTGAGGATCTGGCAGCTACTCCCTGCAACCTGGGGTCTTCAGCCGGACTGGGAATAGGAAGATTTGTCTGCATTGTAAGTGCCCGCGGGAGGAACACAG
CGCCACGGCGATGCCATTGGAAATGGAGAAAACTATGAACAAGCTGATGTTTGACTTTCAGAGGAATTCCACCTCCGATGATGACTCCGGTTGCGCCCTG
GAAGAATACGCCTGGATTCCTCCCGGACTGAAACCAGAACAGGTTCACCAGTATTACAGCTGCCTACCAGATGACAAAGTCCCTTACGTCAATAGCCCGG
GAGAAAAATCCAGAATAAAACAGCTTCTGCACCAGCTGCCTCCACATGACAACGAGGTCCGGTATTGCAATTCGCTAGATGAAGAGGAAAAGCGAGAGCT
GAAACTGTTCAGCAACCAAAGAAAAAGAGAGAATTTAGGAAGAGGCAATGTCAGACCTTTCCCAGTAACCATGACCGGTGCCATTTGTGAGCAGTGCGGT
GGTCAGATCAATGGAGGGGACATGGCGGTCTTTGCATCCAGAGCTGGGCATGGAGTCTGCTGGCACCCTCAGTGCTTTGTGTGCATCATCTGCAATGAAT
TGCTGGTTGACCTTATCTACTTCTACCAAGATGGAAAGATCTACTGTGGCAGGCACCACGCCGAGTGTTTGAAGCCTAGGTGTGCGGCATGTGATGAGAT
TATTTTTGCAGATGAATGCACAGAAGCTGAAGGAAGACACTGGCACATGAAGCACTTTTGTTGTTTTGAATGTGAAACTGTCCTGGGAGGCCAGCGGTAT
ATCATGAAAGAAGGACGTCCATACTGCTGCAACTGCTTTGAGTCTCTCTACGCCGAATACTGCGACACGTGTGCACAGCATATAGGTATTGACCAGGGAC
AGATGACCTACGATGGCCAACACTGGCATGCAACCGAGAACTGTTTCTGTTGCGCTCACTGTAAAAAATCCCTGCTGGGACGGCCCTTCTTGCCCAAACA
AGGGCAGATTTTTTGCTCCAGAGCATGTAGTGTGGGTGAGGATCCAAATGGATCGGACTCTTCAGATTCTGCCTTTCAGAATGCAAGAGCAAAGGAATCC
CGGCGTAGTGCTAAGATTGGAAAGAATAAAGGCAAGTTAGACGATGGCAACATGAACCAGTGTGGGCCCTTACACGTGGCTTCTAACAGACTTTCTGCTG
ATGTGGACCCCCTTTCTCTGCAGATGGATTTGCTGAGTGTATCTAGCCAGAACCCAAGCTTAAACAGGGATCAGCTATGGAGGAGCAGAGATGAGCTGTA
TCACTATGGCAACAAGATCGAGAGAAGCCAATCACAGAGTCCCTTGCAACTTCTTAGTCAGTGCAATATAAGGACTTCTTATACACTGGCCAGGGGGCTG
GGGCCCAGCCTGATATGTGGGCAAAACATTTCAACAACCAGAAGAGAAGCCTGTCCATGATGGGCCACAGTGAAAGCTTCTCTCAGGAAGGCAGGGAAGA
CTATTATCAAGGCAGGGTGAGGCAACAGGAGAGCTTCAGCGACGCGTCCAACCACAGTTTCAACGAGGCGAGGTCCAGCCTCAGAGTTCCGAAGTTTGAG
GAAAAGAAACCTATGAACCCACAGCAGTGTCGATCCAGGCATCCAATCAATGCACTTAAATTCACAGAGGGGTTGACCCCTACGGAACAAACTCCCCGCG
GATCAATGGAATCTCTAGCCCTCTCCAATGCCACAGGTGTATCTGCAGATGGGGGTCCTAAACGTCAGGAACATCTGTCTCGATTTTCCATGCCAGACCT
GAGCAAGGATTCAGGGATGAATGTTTCCGAAAAACTGAGCAACATGGGAACGTTAAACTCGTCTTCTCAGTTCCGGAGTGTAGAGTCCGTCCGAAGCCTT
CTGTCTGCGCAACAGTATCAAGATCTGGCTCCGAATCTTAGTGACTTGGCTAACTCAGGCAAATACAGGGATGTGCCACCCCATTCAAGGCTACATCATG
GATTTACATTTGATGCAGGGATGTCCATGAATGAATTGACAGCCCAGAACAACCTAAAGTTGACTCCAATGAGTGAGAGGACAAGAAGGAGGACTCAAGC
CCGTGATAATGACCAACACTATCGACATCATCGTCAGAGAAGGTCAAGGCGTTCCCGTTCTGATAATGCCCTCCACTTGGCCACTGAACAGTGTAATAAA
ATCAAGGAGAAGCCACTAGCAAGAACTAGGGAGGAATATGAGCGGTTAATGCAACAAAGGGGCGTTAGAGATTCCATGGATCAAGCCGTTCGAAGAGAAA
TGTACAACCGTTATCAAAGGACTGCGTCTGACCTTGCTCTACAAAACCATCTCTATGACAGATGGGGGCAGCACGTCAACGACTATGACTGGTGTTCCAC
CTGCTCCTCCTCATCAGAGTCTGACAATGAAGGATATTTCTTAGGAGAACCCATTCCCCAGCCTACTAGACTCAGATACGTTACTAGTGAGGACCTTATC
CATCAGTACGGCCAATGCTCTGGAGGCATACCCAAGTCTTCCACTTTAGGTGGCCGAGGACAATTGCACAGCAGAAAGAGGCGAAAAAGCAAAAACTGCA
TCATCTCATAGGGGCGAACCGCGTTGCGGCGGCACAGAAATGCGCACGAACAACAAACAGACTTGCACTGCTCTCCATTATGAACTTTATTTTATACCAA
ATTATAAAAAAAAAGTGCAAAATCCCTTTATTTAAAACTTCATTCTGAGGTTTAATATTAGAGTCTAAATTAAGAAACCGAGTTGACTAATCTTCATGGT
CGCCCAATCCCTCGGGTCCACACGTTTCACTTGGATGTAAGTGCCTGGTTTAACTCTCCGTGTAACCAACTTCAATGCAGGAGAACCAACTCATTGGCGT
GTAATCACCTTATCGGGGCAAACTACAGCCAATCGGAGCTTACGCATTTCAAACGTGACAGAGCCAACTGCCATCAGAGCATTTACAAGATGATATCCGT
TGAATCAATCCAAGAAATTTCACCAATCCGGCAAATTTTTGTTTATAACCGTGTTTTCGCCACTGACAAACCGATATAAAGTTCAGGAGGCCTGTAGAAT
AAATAAGCCCCATTATAGTCCATATTTCTTTTTCATTTTTCATCTTTTTCTTATGTTTTTTTTTTGCACATTTCACTTTGAAAGTGACTCTCACTTTGCT
ACTTACCTAGTTCAGTCCGCGAACCTCACTGCCAGGCTTCTGTACAGACCAACACGTTCCAGATTGAATGTACGCTGTATCCAAATGTATGCTGCTTGCC
TGTATCATTCTATAATAGACCTCGACGCTTATTATCATTATTTTTTTCATTGGCCACTTATATTTTGAAGTATTTTATTTCCTAGACAAAAAAAAAAAAA
AA
5' EST: CX825583 [+]aacaaagctgtggttctgaaagagaccctttaaacagcacagcccttctccaaagagggcaggaatcactctctactgctgatgcttctcaacagaacac
attcgcttccagccagcacaatgctcggtgtgcggggctgcttggagaggctttgactaaagccggctgctcatttcaactgtccctgggtctgtcatgc
tgggagagcagaggtaacccccttgtgtcccctgtgcacagccgagatgtttgttgtgccggcaacatggaaaagagaatcaaactgtaaaaactaagga
gcttgaggatctggcagctactccctgcaacctggggtcttcagccggactgggaataggaagatttgtctgcattgtaagtgcccgcgggaggaacaca
gcgccacggcgatgccattggaaatggagaaaactatgaacaagctgatgtttgactttcagaggaattccacctccgatgatgactccggttgcgccct
ggaagaatacgcctggattcctcccggactgaaaccagaacaggttcaccagtattacagctgcctaccagatgacaaagtcccttacgtcaatagcccg
ggagaaaaatccagaataaaacagcttctgcaccagctgcctccacatgacaacgaggtccggtattgcaattcgctagatgaagaggaaaagcgagagc
tgaaactgttcagcaaccaaagaaaaagagagaatttaggaagaggcaatgtcagacctttcccagtaaccatgaccggtgccatttgtgagcagtgcgg
tggtcagatcaatggaggggacatggcggtctttgcatccagagctgggcatggagtctgctggcaccctcagtgc
3' EST: CX825582 [+]aggaaataaaatacttcaaaatataagtggccaatgaaaaaaataatgataataagcgtcgaggtctattatagaatgatacaggcaagcagcatacatt
tggatacagcgtacattcaatctggaacgtgttggtctgtacagaagcctggcagtgaggttcgcggactgaactaggtaagtagcaaagtgagagtcac
tttcaaagtgaaatgtgcaaaaaaaaaacataagaaaaagatgaaaaatgaaaaagaaatatggactataatggggcttatttattctacaggcctcctg
aactttatatcggtttgtcagtggcgaaaacacggttataaacaaaaatttgccggattggtgaaatttcttggattgattcaacggatatcatcttgta
aatgctctgatggcagttggctctgtcacgtttgaaatgcgtaagctccgattggctgtagtttgccccgataaggtgattacacgccaatgagttggtt
ctcctgcattgaagttggttacacggagagttaaaccaggcacttacatccaagtgaaacgtgtggacccgagggattgggcgaccatgaagattagtca
actcggtttcttaatttagactctaatattaaacctcagaatgaagttttaaataaagggattttgcactttttttttataatttggtataaaataaagt
tcataatggagagcagtgcaagtctgtttgttgttcgtgcgcatttctgtgccgccgcaacgcggttcgcccctatgagatgatgcagtttttgcttttt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: