XB-CLONE-3309581
Clone Name: IMAGE:7655560
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Gene:
il1rapl2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121469
[+]
GCTTCACTAGCACCACAGCACTTTATAGGACTCCAACGGGACCGTCACCCCCTACATTGTGGATGTGTTCCCTCTCCCGCTTGTGTGTAACTATGGGATG
ACCGGTGCATATTGGGCGCTACTATACTTACTTAGGGGGGTTACAAGCAAACACGTGGTTGTGGGATCCGCACCTGCCGAGTGAGTGAGGGGGCGATGCG
CACTCGGACACTAATCGCAGACAAAGTGGCGATGTGATTGATGTGATTTCTCAGGGACAAGGGCCAGTATTTACCGCTGTCATGGGATGTACAAGGACCA
GGTTGTAAATGAGTTCTCTCCTACCAAACAAAAGCAGTCTGACCTGGGAGATTTATCGCAGTCGGTGGGAGACACTTCTCATACAAAGTTACATGAAATT
GACTGAGTGTTGGGACATCGGGGAGGAATGAAGCATCGTGTCGTATCAGTCTAGCGCTGGGTGTATGGCTGTCGCTACCGACACGTACTTAGCAACTTTA
TGGGATTGATCAGAGATTCCTGCACCTAATGCGCTTTGGAAATTCACTGCAGACTCTGGGAAATCCAGTGAAAGATGAAGCCTCCAGTATATATGGCTCT
TTTGATCTGCTCAATAGTAAGTGCTGATGTGAAGCTCGTGTCAAAAAGAAACTCAGTGGACGGCTGCATTGACTGGTCAGTGGACTTAAAGAAATACATG
GGATTGGCAGGTGAACCTGTGAGAGTGAAATGTGCCCTCTTCTACAGTTACATCAGGACTAATTATAGCATGGCTCAGAGTGCTGGTCTCAGGCTTATGT
GGTATAAAACCAAAGGAGATCTGGAAGAACCAATCATATTTTCTGTTGTTAGAATGAGCAAGGATGATGATTCTATATGGATTCATACAGCAGAAGTGCA
AGACGGTGGACTCTACACCTGTGTTCTAAGAAACGCCACTTACTGCATGAAAGTGTCAATGTCCCTAACAGTGGCTGAAAAGGAGATGGGTCTTTGTTAT
AACAGCACAATTCGATATCTGGAGAAAGCAGAAGTTACAAAAAAGAAACAAATAATATGCCCAGATATAGATGATTATAGAACACTAAATGAAGACATAG
AGGTGGCGTGGTACAAGGAATGTAAGCCAAAGAAGTGGCGAAGCTTGATTATTCAGAAGGGGAATAGTCTATTGATACAAGACGTGCAAAAAGAAGATGG
GGGAAACTATACTTGCGAACTTAAATATGAAGGCAGGTTGATTAGACGGACAATTCAGCTAAAGGTAACAGCTTTATTCACCACCAGGCCTCCCAAGCCA
TTGTTCCCTGTGGAGAACCAGCCAAGTGTTATAGATGTCCAGCTGGGTAGCCCACTTAGCGTATTCTGCAGAGCATTTTTTGGATTTAGTGGAGAGTCTG
GACCCATCATCTATTGGATGAGGGGAGAGAAATTTATTGAAGAACTGGAAAATCATATTAAAGAAGGAGTAATAAGGCTACTGAAAGAGTATCTTGGAGA
AAAAGAAGTGGAGCTGGCACTAACGTTTGATGCTGTGCAAGAAGCAGATCTTGGTAACTACACTTGCCATGTAGAAAACCGCAATGGACGCAAGCATGCC
AGCATCATCCTACGGAAAAAAGATTTAAAGTACAACATTGAACTTGCCGGAGGGCTGGGTGCCATTCTACTTGTGTTTATCGTGCTTGTCTCTGTTTATA
AATGCTACAACATAGAGCTAATGCTCTTCTACAGACAGAACTTTGGAAGTGATGAAGCTGCAGAAGACAACAAAGAATATGACGCCTATCTTTCATATAC
CAAAGTGGATCCTGATTCATTAGATAATGAAAGCAAGGAGGAGGAGCAATTTGCACTAGAAATCCTTCCTGATATTCTGGAGAAACATTATGGATACAAA
CTCTTTATTCCTGATAGAGATATGATTCCAAGTCCAACGTACATAGAGGATCTTGCCAGATGCGTTGAGCAAAGTCGTAGATTGATCATCGTCCTGACCC
CAGACTACGTGATCAGGAGGGGATGGAGTATCTTTGTGCTGGACAGTCGCCTGCAAAATATGCTGACCAGTGGAGAAATCAAAGTTATATTCATAGAATG
CACTAATTTGAGAGGAAAAGTGAACTATGAAGAGGTGGAGTCCTTAAAGCATGCCATCAAACAACAGTCTTTAATCAAATGGGATGGTCCTAAAAGCAGC
AAGATGAATTCAAAGTTTTGGAAGCGTCTAGTCTACGAAATGCCCGCAAAAAAACGAGAGGTGTTATCACGGCAGCATGTTCTGGATAGTGGGGAGCAGT
CCCTTTTTGGAGATCTACAGACTGTACCTTCCATAGCTGTAACAGGGAACTCTGGCACGCTGGCACCATCTCAACTTGATTTGTCTGCTTTCCATCATGC
AGATTCCTTGCAAATGAGACATTTTTGCAGAGCTTATGAATATGAAAAGCCAACGCCACCTTTGCCAGAAGCAACCCTAGGCAATCACCACACATACTGC
AATATACCTTTAACCCTGCTTAATGGCCAGCAGACTCACAACAGTGCAGCAAAGGAGCAGCAGGGTTTCCATGGGAACAGTACATTGATACCATTATCTT
CAAGAGAGCTAAGCTTCACCAGTGATATCTGGTAAAGAAAAAAAAAAAAAAA
5' EST: CX825884
[+]cttcactagcaccacagcactttataggactccaacgggaccgtcaccccctacattgtggatgtgttccctctcccgcttgtgtgtaactatgggatga
ccggtgcatattgggcgctactatacttacttaggggggttacaagcaaacacgtggttgtgggatccgcacctgccgagtgagtgagggggcgatgcgc
actcggacactaatcgcagacaaagtggcgatgtgattgatgtgatttctcagggacaagggccagtatttaccgctgtcatgggatgtacaaggaccag
gttgtaaatgagttctctcctaccaaacaaaagcagtctgacctgggagatttatcgcagtcggtgggagacacttctcatacaaagttacatgaaattg
actgagtgttgggacatcggggaggaatgaagcatcgtgtcgtatcagtctagcgctgggtgtatggctgtcgctaccgacacgtacttagcaactttat
gggattgatcagagattcctgcacctaatgcgctttggaaattcactgcagactctgggaaatccagtgaaagatgaagcctccagtatatatggctctt
ttgatctgctcaatagtaagtgctgatgtgaagctcgtgtcanaaagaaactcagtggacggctgcattgactggtcagtggacttaaagaaatacatgg
gattggcaggtgaacctgtgagagtgaaatgtgccctcttctacagttacatcaggactaattatagcatggctcagagtgctggtctcaggcttatgtg
gtat
3' EST: CX825883
[+]accagatatcactggtgaagcttagctctcttgaagataatggtatcaatgtactgttcccatggaaaccctgctgctcctttgctgcactgttgtgagt
ctgctggccattaagcagggttaaaggtatattgcagtatgtgtggtgattgcctagggttgcttctggcaaaggtggcgttggcttttcatattcataa
gctctgcaaaaatgtctcatttgcaaggaatctgcatgatggaaagcagacaaatcaagttgagatggtgccagcgtgccagagttccctgttacagcta
tggaaggtacagtctgtagatctccaaaaagggactgctccccactatccagaacatgctgccgtgataacacctctcgtttttttgcgggcatttcgta
gactagacgcttccaaaactttgaattcatcttgctgcttttaggaccatcccatttgattaaagactgttgtttgatggcatgctttaaggactccacc
tcttcatagttcacttttcctctcaaattagtgcattctatgaatataactttgatttctccactggtcagcatattttgcaggcgactgtccagcacaa
agatactccatcccctcctgatcacgtagtctggggtcaggacgatgatcaatctacgactttgctcaacgcatctggcaagatcctctatgtacgttgg
acttggaatcatatctctatcaggaataaagagtttgtatccataatgtttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: