XB-CLONE-3308962
Clone Name: IMAGE:7656317
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Gene:
atp2c2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121974 [+]
GCTTTCATCCTCCTCCTTGGGCAGCAGCACCGATTTACCCCCGCACCCCTCCCAGGCCTCGGTGCAGCCCGTTGCACCTTTACACTGACAACTCAGTGAT
GCAGAGCTTTACTTCCCCACCCCCAGCATCCTCCTGTACCCAGTGCACGGAATGTGGGATGGGCAGGCACCTGCCAGAGGGGGGCGGGGCCTCTCTCACC
TGTCCCAGGTATAGTCCGGGGAGCCCGAATACGCAGGAGCTCTTAGCAGCTCCCACCTCCCCGGACACCCCCTCAGCTGTACCGCCGGAGCCGCAGCTGC
AGGTCACACTGCTACCGGGAGGTATGGGGGGGAAGCGCTTCTCCAAATTGTTGTCCCATATGCGTAGGTTGCCCCACAAATATCACCTTCTGGGAAGCAG
CGAGCTCAAGGCAGAGGCAGATGAACATTGTGAGCTGAAGTCTGTTGAACACGAGGATGAAGTTTTACCTTTACCTGCCAAGGAGGCCAGCAGACTGAAC
GCGAATGAGCTGGTTAAATATTTACAGGTGGATGTAGAAAGTGGCCTTTCAGAGTGCTCCGTGCTTCAGCGTAGAATAAAGCACAGCTGGAATGAGTTTC
AAGTGGATAACACAGATCCTATATGGAAAAAGTACCTGGGCCAGTTTACAAATCCTCTCATTTTGTTGCTGCTCGGCTCAGCCTTGGTGAGCATAATAAC
AAAGGAGTATGAAGATGCAGTGAGCATTACTGTGGCAATCCTCATTGTGGTTACAGTGGCATTTGTACAGGAATATCGCTCTGAGAAGTCACTTGAGGAG
TTAAATAAGCTTGTTCCCCCTGAGTGCAATTGCATTCGTGATGGAAAACAACAGCACCTTCTGGCTCGTGAGCTGGTTCCAGGAGATGTGGTGTGTTTGT
CTGTGGGTGACAGGGTTCCTGCTGACATCAGACTGATAGAGACAACTGATCTATTGGTGGACGAATCAAGCTTTACTGGGGAGACTGAGCCGTGCAGCAA
GACTCATGGCTCTGTAGATGAAGCCAGTGGAATTGCAACACTCAGCAACATTGTGTTCATGGGGACCCTTGTCCGTTATGGAAGAGGGAAGGGACTGGTT
ATTGGAGTTGGTGAAAAGTCTCAGTTTGGAGAAGTGTTCAAGATGATGCAAGCAGAAGAGACTCCAAAAACCCCACTGCAGAAAAGTATGGACAGACTTG
GAAAGCAGCTCTCCCTGTTCTCATTCGGAATAATAGGTTTAATAATGCTAATTGGCTGGCTCCAGGGAAAACCCCTTCTAAGCATGTTTACCATTGGTGT
AAGTTTAGCAGTTGCTGCTATTCCAGAAGGTCTTCCTATTGTTGTTACTGTCACCTTGGCTCTGGGTGTGATGAGAATGGCGAACAAAAGAGTCATTGTT
AAGAAACTGCCAATTGTGGAGACCTTAGGTTGCTGCAATGTCATTTGTTCAGACAAGACTGGGACTTTGACTGCCAATGAGATGACTGCAACCAAATTAG
TAACTTCAGATGGATTGCATGCCGAGGTTACAGGAGTTGGCTATGAAGGAAAGGGATATGTCAGTCTGTTACCATCCAATGAAGAAGTTCATGAATTTTC
CAGTGTATCTATAGGAAAACTTGTGGAGGCTGGGTGTATAGCCAACAATGCAGTTATTCAGAACAACACTCTTATGGGCCAACCGACAGAAGGGGCATTG
GTAGTCCTTGGAATGAAGATGGGCTTGAGCAATGTAAGTGACAACTACATTCGAAAAAAAGAAATTCCTTTTAGCTCAGAACAAAGATGGATGGCAGTAA
AATGCATCGAAAGGCATAACAAGGAAGAGATATACTTTATGAAAGGAGCTTTTGAAGAAGTCATCCAGCATTGTACCATGTACAACAGCAGCGGAAGGTC
ACTACCAGTCACACCACAACAGAAATCTTTATATCTCCAGGAAGAAAAAAACATGGGAATGCAAGGACTAAGAGTTCTTGCACTGGCCTCTGGGAGTGAA
CTTGGAAAACTTACCTTTTTGGGACTTGTGGGCATAATTGACCCTCCAAGAGCTGGTGTCAGTGAAGCAGTTCAGATACTCCTGGAATCTGGGGTCTCTG
TCAAGATGATCACAGGGGATGCCCTGGAGACAGCCCAAGCAATTGGCAGGAACATTGGGTTATCTAATGGACATCTCAAAGCCATTTCTGGAGAAGAGCT
GGACCAGATTGATGAGTCTACACTGTCATCCATTGTCACCAAGGTGTCTGTGTTTTATAGGACAAGCCCAAAGCATAAACTGAAGATCATCAAGGCTTTG
CAAAAGACAGGAGCAATTGTGGGAATGACAGGAGATGGAGTAAATGATGCAGTGGCACTGAAATCAGCTGACATTGGTGTGGCAATGGGTAAAACAGGAA
CAGACGTCAGTAAAGAAGCCGCTGATATGATTTTGGTAGATGACGACTTTTCTACTATTATGAATGCCATTGAGGAAGGAAAAGGTATATTCTACAATAT
TAAGAATTTCGTCAGATTCCAGCTAAGCACGAGCATTTCAGCCTTGAGTTTAATCACTTTATCAACAGTCATGAATTTACCAAACCCTCTGAATGCCATG
CAGATTCTTTGGATAAACATAATAATGGATGGGCCCCCAGCACAGAGTTTGGGCGTTGAACCTGTAGATAAAGATGCCATCAAACAGCCACCCCGTAATG
CCAAGGAAGCCATCCTCAGCAGAGCTTTGATACTGAAGATTTTACTCTCTGCTACCATCATCATAAGTGGCACCCTGTTTGTCTTCTGGAAAGAGATTCC
TGATGGAAAGGTTACACCAAGGACCACAACAATGACTTTCACCTGTTTTGTTTTTTTTGATATGTTCAATGCACTGGCATGTCGTTCTCTGAACAAAACC
ATATTTGAGATTGGCCTTTTCAAGAACCGTATGTTCTGGTATTCAGTACTGGGATCAATCCTTGGGCAAATGGCTGTGATCTATATCCCTCCCTTACAGA
AAGTTTTCCTAACACAGAATTTACATGCACTAGATCTGCTGTTTTTAGCCGGTTTGGCCTCTTCCGTTTTCGTAGCATCGGAACTAATAAAATTCTGCGA
ACAGCACTGCTGCAGGAAAAGAACAGTAAAAGTCCTCGAGGAGGAGATTTGAGTCCTTTCTGCTACTGGTGTTTCCCTGTATAACAAGTTATTGATGACA
CTGTGACCCATGATGTGATTTCCTTTTATGTCACTTGGCAGCGCCGTATGATATTTCCTGAGTATTTATGTACAGTCCAGTCTGCTGTTTATAAGAGAAA
ACCTCCATTATCTGGCATGATTAGCAACAGAAAGACTTGCAAGAACTCATCACACAAACCTACCACATCTTCATCATTTTTTTTTGTACTTTATAATTCA
TTTGAAAATAAAGACTAATCATTTATTTTTTAAATAAAAAAAAAAAAAAAA
5' EST: CX826880 [+]ctttcatcctcctccttgggcagcagcaccgatttacccccgcacccctcccaggcctcggtgcagcccgttgcacctttacactgacaactcagtgatg
cagagctttacttccccacccccagcatcctcctgtacccagtgcacggaatgtgggatgggcaggcacctgccagaggggggcggggcctctctcacct
gtcccaggtatagtccggggagcccgaatacgcaggagctcttagcagctcccacctccccggacaccccctcagctgtaccgccggagccgcagctgca
ggtcacactgctaccgggaggtatgggggggaagcgcttctccaaattgttgtcccatatgcgtaggttgccccacaaatatcaccttctgggaagcagc
gagctcaaggcagaggcagatgaacattgtgagctgaagtctgttgaacacgaggatgaagttttacctttacctgccaaggaggccagcagactgaacg
cgaatgagctggttaaatatttacaggtggatgtagaaagtggcctttcagagtgctccgtgcttcagcgtagaataaagcacagctggaatgagtttca
agtggataacacagatcctatatggaaaaagtacctgggccagtttacaaatcctctcattttgttgctgctcggctcagccttggtgagcataataaca
aaggagtatgaagatgcagtgagcattactgtggcaatcctcattgtggttacagtggcatttgtacaggaatatcgctct
3' EST: CX826879 [+]aaaaaataaatgattagtctttattttcaaatgaattataaagtacaaaaaaaaatgatgaagatgtggtaggtttgtgtgatgagttcttgcaagtctt
tctgttgctaatcatgccagataatggaggttttctcttataaacagcagactggactgtacataaatactcaggaaatatcatacggcgctgccaagtg
acataaaaggaaatcacatcatgggtcacagtgtcatcaataacttgttatacagggaaacaccagtagcagaaaggactcaaatctcctcctcgaggac
ttttactgttcttttcctgcagcagtgctgttcgcagaattttattagttccgatgctacgaaaacggaagaggccaaaccggctaaaaacagcagatct
agtgcatgtaaattctgtgttaggaaaactttctgtaagggagggatatagatcacagccatttgcccaaggattgatcccagtactgaataccagaaca
tacggttcttgaaaaggccaatctcaaatatggttttgttcagagaacgacatgccagtgcattgaacatatcaaaaaaaacaaaacaggtgaaagtcat
tgttgtggtccttggtgtaacctttccatcaggaatctctttccagaagacaaacagggtgccacttatgatgatggtagcagagagtaaaatcttcagt
atcaaagctctgctgaggatggcttccttggcattacggggtggctgtttgatggcatctttatctacaggtttcacgcccaaactctgtgctgggggcc
catccattattatgtttatcc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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