XB-CLONE-3308581
Clone Name: IMAGE:7656456
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Gene:
ankrd6
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154670 [+]
CTTTCCAGGCTGCCCCGATGAGTGCTGCGCTGCCTGAGTGCATAGCAACAGGATAAAAAGATGGCATAAATCCTGCTCACTGAATGTGTTCCATCCCCCA
AGCCTGTAACCTCTGCACTGCTTTGGGAACTGTGTTTATGAATGAACAAAACACCTGGCCCCAGCTTGCCCTGAGAACTTCTCTACTAATTCATGAGCCA
GCAGGATGTGGCCGCTGTGCTTTCAGAACGCCTCCTTGTAGCTGCATACAAAGGCCAAGCGGATAATGTGGTGCAGCTTATCAACAAAGGTGCCAAGGTA
GCAGTTACCAAGCATGGACGAACTGCATTGCACCTTGCAGCCAACAAAGGGCATGTTAACGTTGTCCATATCCTAGTGAAAGCTGGCTGTGATCTGGATA
TTCAGGATGACGGGAACCAGACAGCCTTACACCGAGCAGCAGTTGTAGGAAACAGTGAGGTCCTTGCACTATTGATACAAGAAGGTTGTGCTTTGGACAG
ACAAGATAAGGATGGTAACACTGCACTGCACGAAGCATCTTGGCATGGATTCAGCCAGTCTGTCAAACTGCTCGTTAAAGCTGGTGCTAATGTGCTTGCC
AAAAACAAGGCAGGTAATACACCTCTGCACCTGGCTTGTCAGAATGGCCATTCTCAAAGCTGCCGGATTTTATTGCTTGCGGGATCTCGGGCAGACCTGA
AAAACAATGTGGGAGATACCTGTTTACATGTTGCAGCACGCTACAATCACCTTTCTGTCATCAGAATACTTCTCAGTGCTTTCTGCTCAGTCAATGAAAA
AAATCAGGCTGGTGATACTCCACTTCATGTTGCTGCTGCACTAAACCATAGAAAAGTTATCAAAGTTCTTCTTGAAGCTGGAGCTGATGCAACTCTCCTT
AACAATGCTGGTCAGATAGCTTTGGATACAGCAAGACACCACAATAACTCAGATGTGGCTCTTCTACTCACAAAAGCACCACAGGTTTTGCGCTTTAGCC
GTGGGAGAAGCTTACGGAAGAAGAGAGAGAAGCTGAAAGAAGAAAGAAGGGCTCAGTCAGTACCAAGAGAAGAACTTGGAGAAAGTAAGGCCAGTATTTC
AGCAGCTGATGATACTCACAGTAGTGACCATGCAGCTTTCAAGCAAGGCGATGGCAAGGAAGAGCATCAAACTTCTGCAGCAGGTTCCAAGCAACGGAAA
AGCAGAAAGCCTAAGGCAAAGGAAAAGATTTCTGCTCTCTCAGACCCCACATCACCAGCAGATCAGCAACTATTATGTGGTGCCCAGCAGTCCTCCCATA
ATCATAAGCGAAGGAACAAACATAGAGGTCCCTCTCCTCCATTCCCTCCCCATGAAGCCAGAGCATACCAACTGTATACCTTGTACAGGGGCAAAGATGG
CAAAGTAATGCAGGCCCCTATCAATGGGTGTCGGTGTGAGCCACTATTGAGTAAACTAGAAAATCAGTTGGAAGCCACCAAAGAAGAGATAAAAGCAGAG
ATTGCTACTGTGCAGGGCCAAATGAATGCAAAATTGGGACAAATGGACAACAAAACCCAGCACCAGCTGCGGGTACTAGACAAGCTTATGTTGGAGAGGA
TGTCTGCAGAAAAAGCAGAATGTGTCTGTCGCCTACAACATCATGCAGATGTACAGAAGAATGAAGCGGAGAAGCGGCAGGTATCTGTTGTAAATGAACT
CAAAACGTGGTGCATACTAAAAATTCAGAATCTGGAGATGAGGTTTTCTGGCGATGCCAGGTCAGTGAAGGCTAAATCCATTCCTTCTACTTGTGAATCC
ACTGATCAACCCATTTCAGAGAGTCCCAGCAGCACCAAGGAATGTAACAGTACAGAATCCTCAACTCTTCCACAGTCTTTTGCACTGCATTCCTGTGTCA
CCTTTCAGAGCAGCTTGCCTGATGAGCCAGGAAGGACTGACGCATTCCATTCAGAAGAAAGTAACAGAAAGCAGCAGTTGCCACCTCAGCAAGAGCTGAA
TCTTTGTCCAGCCACAGACCAGATTCCAGCTGCCCCTCTAGCAGTGAGGCCTAAGGAAAAAGGAAGTGCCCACCCTGCCTTAAAAACACAGCAGGGACAG
AATTCATGTGATCATCTTGGTTCCAGAACTCGACAAGTGAAACTTCCATCCATTTCGAAACCCTCAGCAGAACCTAATAAAACTGAAAAGCAGTGTATCA
ATCTCATGGACCGAGGGACTCAGACAAGAAAGTGTGCAAAAGTTATTCATGGGAGGCAAAAAGGACAAATGCCTGAGCCGCCAAGTCAACCGCAGCATTC
TCTCCCACAGGCAAGGGACACATCACAAGCCTTGGAAATCACTCAATATTTCTTTGAGGCAGTTTCTACTCAAATGGAAAAATGGTATGAGAGGAAGATA
GAAGAAGCCAAATCTCAAGCAAATGTAAAAGCAGAGGAGGATAAAGCTGCCTTGCAGCAGCATATTGAGAGTTTAGAGGAAGAACTAAGAAAACTGAGGA
CTAAACTTCAGAAAGAGAGCTAGCATCAACTGTTTAGAGTGAATGTATTCTAAACATGCATGAACCAAGTGTATTATACTAAAGCATACCCCAGTTATCA
GAAGGACTAATTCCCTTTTGCTGTCTGCATAGTGGCTCCACTACTTATGTGATATAAATAATTGTGTTACATGGTGTAAATGTAGAGTTAAAACATTAAG
TTAAGCTTTTCATGCTCTAATCGGTTCAGATTTAAGAATAATGCAGTACTGCATTTACAATTGTGAAAATACGAATAAGAGAATGCAAAAGTATATCTAT
AAAATCCTTCAGAATGTGTTTGCATTGAATCAGTTTATATATAGTAGCATGCTTCTGACATTGCTTTGACAGCTGGGATATTTTGTTTTCTACTATTGTT
GACTTTCAGATACATTTTTTAAGTGCCATTGAATCATCCTGTTGGGTATTATAGACATATATATACATACTAGCAGTCATAAAAACGAACATGTGTAGCT
GAATAGATACGTTTAATGTCAATGTGATTACTTATCTTACACATAAGCAAGGCATGGTCATTCAGGTCTGACTGACATTTCTCACCATCCCATCGACAGT
TAAAAGGTTGTCAGGTATAGCTGAGAATTGTAGTTCTATTACAGGCTTATGTACTATCAACAGTTACAGGCTTATGTACTATCAACAGTGAATTACTTAG
ATAATTCCAGTGTATGCATCTGTTTCTGATACATTCCTCATATTGACTGATATTTTTATTTTACTAGATATTAAAGTATAGTTGTTTATATTTGTAAATA
AACATACCAAGGCTGATATCCTAACATAGGTGGTAAAGTGCACCAGTACGGTTACTCATAGCAACCATCCATTAATGGTTCTGATCAACCTACTTCAAGT
GTGAGTAAATCAGCCCATCATGCAGTTTCAGAGAAGGTCGCTTGGTAGAAATATGTCACATAAGGATATTACCATCTTGTTAATTGCTTGTACTGTTATT
TTCCTAGTCACACAGTGGTTTTCCAAAGTAACATGGTAGATATTAAAAAAATAAAATTAATCTGAAACATTCAACTGTGTAACAATAAAAAAACAACAAT
TCACGGATAAAAGTGGTTTTATAACACAAATTTGTCACTTTGCCAAAATTTAGATTTGTTTACTGCTAGCTAATATCATCAGCCGCATAGAAAATTAAAA
GTATACTTATTCACAATGTGGCCAAAAGTATCTGGGTACCTGCCTGTCCAACTTCTCATTCCCAAACCAAGGGTATTCATATGAAGTTTGTCCTCCCTTT
GCTGTTATAATAGCCTCTATTCATCTATTCCACTACATTTTCTAACATTTTTGGTGAGATTTGTTTTCATTCAGTGACAAGAACCTTTGTAAGGATAAGC
ACCAATGTTGTGAGGCCTGTTAATTGGCATTACAGTTCATCCTACAAGGGGCAATAGTGTTTTTTGCCTCTGACCACTCATAAAAAGCTCCAATTTGTTT
CCAAATTCTGCAGTAAGTGTTCCAACATGCAATTTTACATGGGGAGTTTGAATACTTTTGGCCATACAGTATTATATATATATAGGAACAACAAACAAAT
GGTTGCAATGCACTCCCAATATAAGGGAAAATGGCCTAGGTGTATCCAAGAGAAATGAATATCCACATGAAAAAATGGAGCACTCATACTTGTGAATGAG
TGGGAAGGTTTTCATTGGTTTGTCGCTGTTTCAGTCCTCCTTAGAGGACCAAGACGTAATGAGAAATGTCCTCTCGGCAGGACTGAAACATTGACGGGCT
GGTGGAAACTTTTTAACTCATTTTAAGTCCCATAAGTACTCAATTTTTTCATCAACACCTGTGCAGTGCTGAGTCCTTTTGGTTTTCTATTCTTTGTAAC
TCTGCCTGCACTCCTCTCCTGCAACAAATGCCAGGTGCAAATCAGACAGAAAATGTTGCCTTCTTTTACTCCTACACACATAAAAATAAGAAATACTCTG
ATGCACACTGGTCTTATGTTAAAGTGCAAAAAAGGCGTCCTTTATTTGAGTCAATGTTTTGACACCACATAGGGGTGTCTTCCCCCAGGACAACCAACCT
TGTTGACTTGAATGGAGTAGGCTTTTTGTGTTTTGATGTAGGACCAATGTGCATCAGAGTATTTTGTATTTAAATATATCAGAAAAAAAAGAAGCCAAAT
TTGCCAATTATTAGTTTTACTATCATAGTGGAGGCTCACATTCAGATTAACCTATAGTGTTATTGATTAACCTACTGTAAGAAACTATACTTTTGGTCAG
TATTTATTTGTCATGGCCGACTCTCACACTATTATCTCCATATTATAACATTCCATATATGATACAATATAAAACTGATAAAATACAAACATAATAGGAC
TTATTTAACAACAGGGGGTAAGAATCTGTGATTTTTTCCACCATGCACTTGGCTGGTATGTCATGCAGCATAAGCATTATTCAGTGTGGGTTGAGTAATA
ATTCTGGGTTAAATCTATGATTGTTTTCCAATTTGGGAGCTTGGGGTTTGACATGGAAGTGTGTGCCTCTGATTTTTTTTCAAAAATAAAACCAGAATAG
AAAATGTTGTAGAAAAATATGTTGGTTTCTAGATGAGAAATGATGTGTAATAACATCAAATCTGCTAGATTCCATCTATGGTTTAGATGATTTCACCCCG
CTTGATTTTAATATCTGGATGAGGAGGTACATATTGCCTTGTTAACCAATACTGTCTTACAGAAAGTGCATTCTTACCTTTCTTGTCATCTGTGCAATTC
AGACTCAGTTACATGCCTTTTTGTATTCTGCAACATTACCTCAGTATTTATATGGATTATATTTAGAGTGTATTATGCCTTGTGCTGCTTCATTTTAAAT
GTAATAGTATTTATTAGTTTTTATTGCATCTAGTAAGATTTTGTTAGTACTAAAATAAATATTACAATCATAACCATAAAAAAAAAAAAAAA
5' EST: CX827548 [+]ctttccaggctgccccgatgagtgctgcgctgcctgagtgcatagcaacaggataaaaagatggcataaatcctgctcactgaatgtgttccatccccca
agcctgtaacctctgcactgctttgggaactgtgtttatgaatgaacaaaacacctggccccagcttgccctgagaacttctctactaattcatgagcca
gcaggatgtggccgctgtgctttcagaacgcctccttgtagctgcatacaaaggccaagcggataatgtggtgcagcttatcaacaaaggtgccaaggta
gcagttaccaagcatggacgaactgcattgcaccttgcagccaacaaagggcatgttaacgttgtccatatcctagtgaaagctggctgtgatctggata
ttcaggatgacgggaaccagacagccttacaccgagcagcagttgtaggaaacagtgaggtccttgcactattgatacaagaaggttgtgctttggacag
acaagataaggatggtaacactgcactgcacgaagcatcttggcatggattcagccagtctgtcaaactgctcgttaaagctggtgctaatgtgcttgcc
aaaaacaaggcaggtaatacacctctgcacctggcttgtcagaatggccattctcaaagctgccggattttattgcttgcgggatctcgggcagacctga
aaaacaatgtgggagatacctgtttacatgttgcagcacgctacaatcacctttctgtcatcagaatacttctcagtgctttctgctc
3' EST: CX827547 [+]tttttttttttttttatggttatgattgtaatatttattttagtactaacaaaatcttactagatgcaataaaaactaataaatactattacatttaaaa
tgaagcagcacaaggcataatacactctaaatataatccatataaatactgaggtaatgttgcagaatacaaaaaggcatgtaactgagtctgaattgca
cagatgacaagaaaggtaagaatgcactttctgtaagacagtattggttaacaaggcaatatgtacctcctcatccagatattaaaatcaagcggggtga
aatcatctaaaccatagatggaatctagcagatttgatgttattacacatcatttctcatctagaaaccaacatatttttctacaacattttctattctg
gttttatttttgaaaaaaaatcagaggcacacacttccatgtcaaaccccaagctcccaaattggaaaacaatcatagatttaacccagaattattactc
aacccacactgaataatgcttatgctgcatgacataccagccaagtgcatggtggaaaaaatcacagattcttaccccctgttgttaaataagtcctatt
atgtttgtattttatcagttttatattgtatcatatatggaatgttataatatggagataatagtgtgagagtcggccatgacaaataaatactgaccaa
aagtatagtttcttac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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