XB-CLONE-3308535
Clone Name: IMAGE:7656801
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Gene:
plk2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136055 [+]
AGTCCCGCAGCGGCAGGAGTAGGTTGGCTGAAGGGTTATGGAATTACTGAGGAATATCGCTTATCAACCAAGCAGCGGGGGCAAGATGTGCGAACAGGCG
CTGGGCAGGGTGTGTGACAGCAACAGGAGGTGGAAGGTACCGGCCGATGGGGAGCCCATTCATCACACCTGCTCTGCCACTGAGGTCTCCAGGATTATAA
CCGACCCTGGCACAGGGAGGCGCTACTGCAGGGGCAAAGTGCTTGGCAAGGGTGGATTTGCGAAATGCTATGAAATGACAGATTTGACAACAAACAAAAT
ATATGCTGCTAAAATCATCCCGCACAGCAGAGTGTCCAAGCCTCACCAAAGGGAAAAGATTGATAAAGAGATCGAATTGCACAGAACCCTGAATCACCGG
CACGTCGTACAGTTCTATCACTATTTTGAAGACAAAGAGAACATCTACATATTACTGGAGTACTGCAGTAGACGGTCCATGGCCCACATTCTAAAGACAA
GAAAAGTGTTGACAGACCCTGAAGTGAGATACTACCTCAAGCAAATTGTCTCTGGTCTGAAATATCTCCATGCGCAAGAGATCCTCCACCGGGATTTAAA
ATTAGGAAACTTTTTTATTAATGAGAACATGGAACTGAAAGTGGGTGACTTTGGCCTTGCAGCCAGACTGGAGCCTCTGGAGCAGAGAAGAAGAACGATT
TGTGGCACGCCAAATTACCTCTCCCCCGAAGTGCTTAACAAACAAGGGCACGGCTGTGAATCAGACATCTGGGCACTGGGTTGTGTAATGTATACAATGC
TGCTTGGAAGGCCGCCGTTTGAAACCACAAATCTTAAAGAAACATACAGGTGTATTCGGGAAGCAAGATATTCCCTGCCTTCATCGTTGATGACGTCTGC
AAAGCATCTCATAGCAAGCATGTTATCCAGGAATCCTGAGGACAGGCCCAACCTAGATGAAATTACGCAACATGACTTTTTCACACAGGGTTTCACACCA
GAGCGACTGCCACCTAGCTGCTGCCACACGGCTCCTGATTTCCACTTATCAAGTCCAGCTAAGAATTTCTTTAAAAAGGCCGCTGCAGCACTGTTTGGAG
GGAAGAAAGAAAAGTCAAAGTATTTGGATAATCACAATAAACTGCCAAAGGAAGATGAGGAAATATACAAGCTGAGACAGGACCTACAGAAAAGCTCCAT
ATCTCATCAGCTGCACAATCCCAGAACGGATGAGGAGATCAAAACCATCTCCAAATTAGATGTTCTTGCAGAGAAGGCAGACAAACAGCAGATGGGAGAT
ACCATCCGCATGATTGTTAGAGGAACACTTGGAAGCTGCAGTAGCAGTAGTGAATGTTTGGAAGATAGCACGATGGGAACTGTGGCAGATACTGTTGCTC
GGGTTCTAAGAGGCTGTTTAGAAAACATGCCTGACGCTGATGCTATCCCTAAGGAACAGATTGACACTTCCTTCCACTGGGTTACAAAATGGGTGGACTA
CTCCAACAAGTACGGCTTTGGTTACCAGCTGTCGGATCACACAGTTGGCGTATTGTTCAACAACGGAGCACACATGAATTTGCTGCCTGACAAAAAAACT
GTGCATTACTATGCCGAGCTTGGACCATGCTCTGTGTTTCCTGCCACTGAGGCCCCTGAACAGTTCATCAGTCAAGTGACAGTTCTGAAGTACTTCTCCC
ATTACATGGAAGAGAATCTGATGGATGGTGGTGACTTGCCAAGTGTAACAGATGTTTGCCGGCCTAGGCTGTATCTCCTTCAGTGGCTTAAATCTGACAA
GGCTCTTATGATGCTCTTCAATGATGGCACATTTCAGGTCAACTTTTATCACGATCACACGAAAATCATAATCGCAAACCAGAACGATGAATACTTGCTA
ACCTATATCAACGAGGACAGGATGTCTACGACATTCCGCCTGAACACCCTTCTGATATCTGGTGGCTCCAGTGATCTGAAGAACAGAATGGAATATGCAT
TGAACATGCTGCTGCAAAGATGCAATTAAGTTGCCTGAGGGCTCCTGGATATCTAAAGAAGACATGTCCTTCACTGTGAGAAACAGACGGCAAGAGAGAT
TGAGATAGAAACAAAGTCTTAGAATGTCGTGTGTTGGAAACAGGAGGTGGTACGAAATGAATTTCCAACGTAGTGCTCCTGGGCTGGGACCAGACTGTGG
CTCTAATCAGTCTACTTTGTTGGATATTTGTAAAGAAAATCTCCTCAAAGGTGTGAACTTGATTGTGGAAGAGAAACAGGAAAGCGTATGGACCTTAGCA
ACCAAATCCGACAAAGTGGGGTGATGTTATGTTTTGCTCCATTGAGAGCACTCTTCTGCAGGACTTGTACTGTGAACATTAAGATTCCCACATGGACACG
TTAGCCCTTTTTTAAAGGACAAGTAGAATGTCTGAAAAGTGTGGAATCTCAATTATAAATGCAGCTGTTAGCTGCCTAACTGTGAACTATGGCCATTTTG
TTATTATTATTATTATTATTATTTCCGTTTAATACCCACATTTGTGGCTGGACAAGTGCATTCCTAGTTATTACAATTATTTATTACAGCCCAAAGTGAA
GTATTATTTATGGTTTTTTTTGGGGGGGGGGACTTAATATTTTAAGTATCTTCTTTATGGCAATAAAGAAAAGTTCAAATGTTTAAAAAAAAAAAAAAA
5' EST: CX827617 [+]agtcccgcagcggcaggagtaggttggctgaagggttatggaattactgaggaatatcgcttatcaaccaagcagcgggggcaagatgtgcgaacaggcg
ctgggcagggtgtgtgacagcaacaggaggtggaaggtaccggccgatggggagcccattcatcacacctgctctgccactgaggtctccaggattataa
ccgaccctggcacagggaggcgctactgcaggggcaaagtgcttggcaagggtggatttgcgaaatgctatgaaatgacagatttgacaacaaacaaaat
atatgctgctaaaatcatcccgcacagcagagtgtccaagcctcaccaaagggaaaagattgataaagagatcgaattgcacagaaccctgaatcaccgg
cacgtcgtacagttctatcactattttgaagacaaagagaacatctacatattactggagtactgcagtagacggtccatggcccacattctaaagacaa
gaaaagtgttgacagaccctgaagtgagatactacctcaagcaaattgtctctggtctgaaatatctccatgcgcaagagatcctccaccgggatttaaa
attaggaaacttttttattaatgagaacatggaactgaaagtgggtgactttggccttgcagccagactggagcctctggagcagagaagaagaacgatt
tgtggcacgccaaattacctctcccccgaagtgcttaacaaacaagggcacggctgtgaatcagacatctgggcactgggttgtgtaatgtatacaatgc
tgcttg
3' EST: CX827616 [+]tttttttttttttttaacatttgaacttttctttattgccataaagaagatacttaaaatattaagtccccccccccaaaaaaaaccataaataatactt
cactttgggctgtaataaataattgtaataactaggaatgcacttgtccagccacaaatgtgggtattaaacggaaataataataataataataataaca
aaatggccatagttcacagttaggcagctaacagctgcatttataattgagattccacacttttcagacattctacttgtcctttaaaaaagggctaacg
tgtccatgtgggaatcttaatgttcacagtacaagtcctgcagaagagtgctctcaatggagcaaaacataacatcaccccactttgtcggatttggttg
ctaaggtccatacgctttcctgtttctcttccacaatcaagttcacacctttgaggagattttctttacaaatatccaacaaagtagactgattagagcc
acagtctggtcccagcccaggagcactacgttggaaattcatttcgtaccacctcctgtttccaacacacgacattctaagactttgtttctatctcaat
ctctcttgccgtctgtttctcacagtgaaggacatgtcttctttagatatccaggagccctcaggcaacttaattgcatctttgcagcagcatgttcaat
gcatattccattctgttcttcagatcactggagccaccagatatcagaagggtgttcaggcggaatgt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: