XB-CLONE-3308198
Clone Name: IMAGE:7656728
|
Gene:
kif5a
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX828142 [+]
gccgagcattgccagcagcatcacccacagctctaacacattccctgccgcacagcttccctgcccccccatcatggctgataacagcattaacactgag
tgcagcatcaaggtcctgtgcaggtttcggcccctgaaccagtcggaggtgcagagaggagacaaattcctgcccatattccaaggcggggacactgtca
tcgttgggggtaagccttatgcatttgatcgggtgtttcctccgaacacaactcaggagcaagtctatcatgcctgcgctatgcagattgttaaagatgt
gctggcagggtataatgggacaatctttgcttatgggcagactgcatctgggaagacccacaccatggagggaaaacttcatgacccacaattgatggga
ataattccaaggattgccagggatatctttaaccatatttatgccatggatgagaacctggaatttcatataaaggtttcctattttgagatttacttgg
ataaaataagagacctgcttgatgtatctaaaaccaatctctcagttcatgaagataaaaaccgcgttccatttgtcaagggttgcaccgagcgctttgt
gtccagcccagaggagatccttgatgtcattgatgaagggaagtcgaaccgccatgttgcagtaacaaacatgaatgagcacagttcccgtagccacagc
atcttccttatcaatattaagcaagagaacattgagacggagcagaagctcagtgggaagctgtacttggttgacctggctggaagtgaaaaggtcagta
agactggagct
3' EST: CX828141 [+]atccaaattaaatatatacagcagttcttattgttaaagaacttacgctataaacacacatacacatatccgccctgtgattttcccctctgtaagaaat
ttcggcttcaaacttacaaatgccaatataatgtcacaaacactgcattgaaatatcatcatttctttattttgatttccctcattatcccatctttatg
gtgatacatcactagcccccatgtcagtgatacaaggtgtgggatttgacagatatatcataaataactttgataggatagaggttaaaacacaagtgga
tacataatcaagtgcattacaaaggtatagaataaacattagcagctgttagtatacagtacataaacagagcacagtagaaagacactgagcgagttat
acagggaaggtaatagttggcagcagaggccacaaaaagctaacttgcagcatgctgggtacaagcttcagcccctttggaaactgaaagtgcacatgat
atgagaatttatatgactgcgttattagctggctgctgtctcctgatgaagagggaacaacttggactgatcctcagcttcacagccacatggcaaatcg
cttctgttatcattgatatctgtagcatttccattatccacattcatcttctgataggaaagcaagctgccactggaagaactgccccgtgtgctggaac
atggattggcaaagaatgtttcactgcttgngctggttgcccgcatgtatatattttggtagttctgaaagaggttgttggtataactgattgagtc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: