XB-CLONE-3308079
Clone Name: IMAGE:7656993
|
Gene:
raver2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC122907 [+]
GCAAAATGGCGACACGGGATACCGGGGTTCCCCTGATGTCTCCTGGGCAGGATGAAGCCAACCCGGACCCCTTTGCTGCAGCCTCCCAGGACTCGCAGGA
GTGCGGGCTAAAGCAGCCTGTGTGTAGTGAGGGAACAGCTGCAAGTGTGCCTGCGCTGGGCGAGCTAGAGATAGCACAGAGACTGGAGGCAACCCGGTGG
GAGCTTGGGAACCGCAGGAAGATCCTCATGAGGAACCTGCCGCAGGACTGCACCAGCCAGGATATCCATGACTTGCTGAAAGGCTATGAAATTAAATACT
GCTACGTGGACAAAAATAAAAAAAACAGCCTTCATCACGTTATTAAATGGAGAGCAAGCACAAAATGTCATTCAGAAGTTCCATCAGTACGCACTCCGTG
ACAGAGACATTTCAGTTCAACTTCAGCCTACGGATGCTGTACTCTGTGTTACCAATTTACCCCCGTTTTTTACATTGCATGACTTTGAAGATTTAGTGAG
AGCTTATGGCAATACTGAGAGATGCCTGCTGGTCTACAGTGAATTTACAGGTCATTCCAAAGGATATGGATTTGTGGAATACATGAAAAAAGATTCTGCC
TCTAAAGCTAGACTGGAATTAATTGGGAAAACGTTGGCAGAATATACCCTTTTTGCACAATGGATGGATAGCAATCAGCTAACCACAGAACTTATTCACT
CAAAGTGCCTGTGTGTTAATAAGCTTCCCAAGGGCTACAATGATTCTAATGAACTAACACAGCTGTTCTCTGCTTTATACAAACCATTGTTCTGCCAGTT
GGCTGAAGATGAAGTTGGTTGTTTTGTTGGTTTTGCAGTGGTGGAGTATGAGACAGCTGAGCAGGCTGAGGAGGTAAGAAAGTCAATGGATGGTACCACT
GTCAAAGGAAGCAGAATCCAGCTGTCCTTCTGTGCCCCGGGCTCTCCAGGCAGAAGCACTTTAGCAGCTCTTATAGCAGCACAAGGAATGCTGCAGAATA
ATAGGAAAGGATTGCTTCCAGAACCAAACCCAGTACAAATAATGCAAAGTTTTAGTAACCCAGCAATGTTACAGATGCTGTTGCAACCCCAGCAAAGAGG
AAGACCGGGAAAGCATGGTTCAACATCAGGACGGCCTCACTTTGTGAATCCGACAGTAAGCCAAGCCTTACTACAACTCAACAAACTGCATCAGACACAA
GGTATGGGAGGTAATTCCCTGCTTCAAAACTTCTCACATCTACAAATGGCACAGAAGCAACTTTTGCAGCTAAAAAGTGCTCAGTCGAATAATTGTAAAC
CTGGCCTTCTCGGAGAAGCTCCAGTCTCCGTGTTACAGACAGGAATGGGCCCATCACAGCCAGGATCAGTGGAGGCTTCGCAACAAGATGGGCAGAAAAG
CATGATGCCTTTCTACCACAATCCGCACATGAGTTCAGTTCTGTCCCGTCTGACACAGGATAAACAGCCGACATCTATAGCTTTGCTGGAAGGGGCAGGA
TCTCAGCCTTATTTACAGCAGTCTGGATCAGTGCTTACAGGGCCGAATCAGCCACAAAACCAGCCTGCTAGCTCTGGGTCACCACTCTTGAATAAATCCA
GTAATCAGACCTCACTTCTGGGTGAGCCTCCAAGAGATATCAGACTGAGTACAAATCCATATCTAAATCTGGCAAGTGTGTTGGCTGGTTCCTCAATAGC
AGGCATGGGCTTACGGAACATTCAGCAACATAATATGAATAGATCAACCACTCAGGAATCGGCATCACAGCCTGGGTTGGAAAGTTATTTTGGCTACAGT
CAGCAATATGGAGATTATTCACAGGAAGCCCTGCAGCAGTGGTACGATCAATATTCTGCTTACAGCAGCAGTCAAGTGGAGCAGTCCCATAGTGAAGAGC
AAGCTGAGCAGCAAAACTCATATACAACTGGAGTTGAAGGAGAGAATTACTATTATCAGTCGTCTTCTGGTTTGGCGTATGGAAATTATAGTGCATATGT
CCAAGCACCACCAACATACTACTCCAGTGCCCAGATGACCTACCAGGCGGAGAGTACAGCGCCATCTAATAAGGCATCAACTAAAGAAAAGAGAGCGTAT
ATTTTATCTGCTACAGAGGCCAGCCCAGCAGGATACATGAATCCGTTAACAGAAGGTTCGGGAGAGCACTATGGCGATTCATACTTTAAAAGGAAGAAAG
TTTATTAATTGTGTGAACTATACAAGTCCCTTCTGCTTCCATCATCACATACCCAAAGATGCACAATGCAGTTCATCTTCTTCTAAGCCTTTTAGCAGGG
AACTAAAGGGTTACATTCAACTTCTCAGGCTGAATTGTTTATTATAAATGGTAAAAATCTTAAGATTTGTAGATATTTTCTACACCGGAATATGTCTTGG
TAAATAACATATTTGATAATATAATAATATTGCTATTGCCTGCAAAAAAAAGTTAATTTGTAAATATTAAGCAATAAAGTTAATAGTAAAGATTTACTTT
TACAAGTGACTTCAAATGGCCTGTGTTTGCCTACAAGCTGATGCACTGATGGACACCTCTGTATTAGCCACTAGAGCCGGCAACATCTTCCAACTGTGAC
TGTGTCTCCAATGGCATGCTTTCCAAAAACTTCCTTACCTCCTGTAGTTTTTGAGCAATAAAAAAAAAAAAAAA
5' EST: CX828331 [+]caaaatggcgacacgggataccggggttcccctgatgtctcctgggcaggatgaagccaacccggacccctttgctgcagcctcccaggactcgcaggag
tgcgggctaaagcagcctgtgtgtagtgagggaacagctgcaagtgtgcctgcgctgggcgagctagagatagcacagagactggaggcaacccggtggg
agcttgggaaccgcaggaagatcctcatgaggaacctgccgcaggactgcaccagccaggatatccatgacttgctgaaaggctatgaaattaaatactg
ctacgtggacaaaaataaaaaaaacagccttcatcacgttattaaatggagagcaagcacaaaatgtcattcagaagttccatcagtacgcactccgtga
cagagacatttcagttcaacttcagcctacggatgctgtactctgtgttaccaatttacccccgttttttacattgcatgactttgaagatttagtgaga
gcttatggcaatactgagagatgcctgctggtctacagtgaatttacaggtcattccaaaggatatggatttgtggaatacatgaaaaaagattctgcct
ctaaagctagactggaattaattgggaaaacgttggcagaatataccctttttgcacaatggatggatagcaatcagctaaccacagaacttattcactc
aaagtgcctgtgtgttaataagcttcccaagggctacaatgattctaatgaactaacacagctgttctctgctttatacaaaccattgttctgccagttg
gctgaagatgaagttggttgttttgttggttttgcag
3' EST: CX828330 [+]gctcaaaaactacaggaggtaaggaagtttttggaaagcatgccattggagacacagtcacagttggaagatgttgccggctctagtggctaatacagag
gtgtccatcagtgcatcagcttgtaggcaaacacaggccatttgaagtcacttgtaaaagtaaatctttactattaactttattgcttaatatttacaaa
ttaacttttttttgcaggcaatagcaatattattatattatcaaatatgttatttaccaagacatattccggtgtagaaaatatctacaaatcttaagat
ttttaccatttataataaacaattcagcctgagaagttgaatgtaaccctttagttccctgctaaaaggcttagaagaagatgaactgcattgtgcatct
ttgggtatgtgatgatggaagcagaagggacttgtatagttcacacaattaataaactttcttccttttaaagtatgaatcgccatagtgctctcccgaa
ccttctgttaacggattcatgtatcctgctgggctggcctctgtagcagataaaatatacgctctcttttctttagttgatgccttattagatggcgctg
tactctccgcctggtaggtcatctgggcactggagtagtatgttggtggtgcttggacatatgcactataatttccatacgccaaaccagaagacgactg
ataatagtaattctctccttcaactccagttgtatatgagttttgctgctcagcttgctcttcactatgggactgctccacttgactgctgctgtaagca
gaatattgatcgtaccactgctgcagggcttcctgtga
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: