XB-CLONE-3307747
|
Clone Name: IMAGE:7657114
|
Gene:
sirt4
|
Species: Xenopus tropicalis
|
|
|
|
Sequence:
Full length sequence: BC161022 
[+]
ggtgtgagaaggagagcaagagcactgagcgcccttaacctgacggggggcggggggggggatcctgccatttacgcagcacgtgacctgagccatttcg
tgacgttacctgcagccacaaaagtctttacctgactctctctttcaacacctaggccatgacgtcatttccgccaacacaggagaccgcgtagcgaagc
ggcaatacaaggctatgacatcacggaagttcctttacggttctggaatgttgaatgtaccccgtactaacaatgatgagtgccgcaacggtttcttcct
gcgctgactgatcatgtgatccgtctcctccctcccctgcaagctatacactatacgcgctgtattccctatgcacgctatacttcacctactgtgagaa
gtgctgcttcccccagcagtcagaaggcaggcagtgcgaggcctggtggaaacttagcatattccttgaaatatatcaagttgataagttgctgcctttt
tgcacataatttggaatatgtggaagaacgtgagagaaggttccaaggtgttctggggaataaacaatataactcgcagccacaaatcccaccttgcatt
gtcagaatttgtccctgcatgtcccccaccaaatcctcatcaagttgaacaacttcaggactttgtatctcaatcccagcgtctgtttgttatgactgga
gctggaatctccactgaatctggaatcccagactatcgctcagaaggggtcgggttgtattccaggaccgaaagaagacctatccagcattcagagtttg
tccagagtcaggctgcaagacggagatattgggctcgtaactttgtaggatggcctagcttctcttctcatgagccaaattcagcacatgtcaatctatg
taagtgggaaagagctgggcgattgcattggctagtgacacagaatgtggatgcattacacaccaaagctgggcagtgccgcttgtcagaattacatgga
tgcactcacagggtgatatgtcttggctgccagacagtgactaaaagatcagagctgcaggaaaggtttctgaatctcaatccatcctggaatgagcagg
cacatgggttggctcccgatggtgatgtctttctgacagatgagcaggtgtcagactttcaggtcccagcctgtaccaagtgtgggggaatcctaaagcc
ccaagtcacgttctttggagatactgtgaaccgagggtttgtgttctccatatatgagcaaatgaaacaggctgatgctatgctgatagttgggtcctca
ctgcaagtttattctggttatcggtttgcacttaatgcgaaagaactacaccttccaattgcaattctcaacattggacccactcgtgcagatcacttag
caaaagttaaagtcagtgcccgctgtggagatgtattgccacacatattgctacaggaccaatggcacaaagaaattcaaagctaattatattcacttgg
ggaatatacaaaatagggaaaccagatgacgtggttttctaattgacttcagataatttaggcccaagatctgaaggcagaagaagagacttaaaacaac
tataaaaaaaggcatacattggaagatcattcaagactatgacccatttgtattcaggtagacagcaatgttgtggttttggcataattccatgttaaag
agcgatggtgaacaaaattacttttctttttatttcaaggtcgtgtgtttttttaaacagtggcttgaattaggtgcatgggcattacagcttgcaagaa
gcactatattatgcatgcacccaggttaagccactgcaagggacacctacctgtgagcacaggaaagttggctacacccttcaacaaggtgcttagggct
ggttccttttttagtagagtagaggcacaccagcccagagtaaatggttcatgactttggtctgcaaagaggcctaacaaattgctattccccagtattt
tcaagtttcttggcttttatggattattaattccattttacatctatagctaatataataatgcacaaaacaagataacttttatcaaaaggagaatgaa
tacttgtttccacttgctgctgcaaatcttaatattgctttatctatttattttgcgtagtgtgtatggatgttactacaggtaagaaatctgttgtcca
tgttatcctgaaacccattatccaaaaagcgctttttccgtaataataaacctttaccttgtacttgatcccagctcagatataattaatccttattgga
ggcaaaacaatcctattgagtttgtttaatgtttaaatcatttttagtagatttaaggtatggtgatccaaattacacagtgattccttatccagaagcc
cccaggtcctaggcattctggataacaggtcccatacatgtatatcacactaagtgaggccctcaaactatagcactgccactttcaagactacctcaag
tttcatgttgtcttcatccagacaatgacatttttttaattgattaataattatcttaaaggagtcatctatcaaaagaaacaggatttattgtctcctt
tttttgtaaacatgttcttagggtatctgacttcctatccttcattcagggggccagggtcttcacagttctctcctcgctcccccctcccataagaatt
catatatcttactccccccaccattaggaatgtgtaatctgagctataattgccaagctgcaagcaggaggctttgaagaccaagctgaaatggcagctg
caatcttgaacaaacagagaaaccttctagggctgtttactcagctatgaaaaagcattctgcagaataaatatagcattctcctttaaagcggacctgt
caccttaagaaataattccaaattgttttctgttgtggttgtcaagcaaaataaactttacttacactatataaaaaaaaaaaaaaa
5' EST: CX828864 [+]gtgtgagaaggagagcaagagcactgagcgcccttaacctgacggggggcggggggggggatcctgccatttacgcagcacgtgacctgagccatttcgt
gacgttacctgcagccacaaaagtctttacctgactctctctttcaacacctaggccatgacgtcatttccgccaacacaggagaccgcgtagcgaagcg
gcaatacaaggctatgacatcacggaagttcctttacggttctggaatgttgaatgtaccccgtactaacaatgatgagtgccgcaacggtttcttcctg
cgctgactgatcatgtgatccgtctcctccctcccctgcaagctatacactatacgcgctgtattccctatgcacgctatacttcacctactgtgagaag
tgctgcttcccccagcagtcagaaggcaggcagtgcgaggcctggtggaaacttagcatattccttgaaatatatcaagttgataagttgctgccttttt
gcacataatttggaatatgtggaagaacgtgagagaaggttccaaggtgttctggggaataaacaatataactcgcagccacaaatcccaccttgcattg
tcagaatttgtccctgcatgtcccccaccaaatcctcatcaagttgaacaacttcaggactttgtatctcaatcccagcgtctgtttgttatgactggag
ctggaatctccactgaatctggaatcccagactatcgctcagaaggggtcgggttgtattccaggaccgaaagaagacctatccagcattcagagtttgt
ccagagtcaggctgcaag
3' EST: CX828863 [+]agtgtaagtaaagtttattttgctggacaaccacaacagaaaacaatttggaattatttcttaaggtgacaggtccgctttaaaggagaatgctatattt
attctgcagaatgctttttcatagctgagtaaacagccctagaaggtttctctgtttgttcaagattgcagctgccatttcagcttggtcttcaaagcct
cctgcttgcagcttggcaattatagctcagattacacattcctaatggtggggggagtaagatatatgaattcttatgggaggggggagcgaggagagaa
ctgtgaagaccctggccccctgaatgaaggataggaagtcagataccctaagaacatgtttacaaaaaaaggagacaataaatcctgtttcttttgatag
atgactcctttaagataattattaatcaattaaaaaaatgtcattgtctggatgaagacaacatgaaacttgaggtagtcttgaaagtggcagtgctata
gtttgagggcctcacttagtgtgatatacatgtatgggacctgttatccagaatgcctaggacctgggggcttctggataaggaatcactgtgtaatttg
gatcaccataccttaaatctactaaaaatgatttaaacattaaacaaactcaataggattgttttgcctccaataaggattaattatatctgagctggga
tcaagtacaaggtaaaggtttattattacggaaaaagcgctttttggataatgggtttcaggataacatggacaacagatttcttacctgtagtaacatc
catacacactacgcaaaataatagataaagcatattaagatttgcagc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
