XB-CLONE-3307725
Clone Name: IMAGE:7656978
|
Gene:
arhgef10l
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC161645 [+]
aaagagcagactggggagccacactggggcctctctcatcgctgccttagggattctgtttctttctccttttgttgctggataagggaattctgggtgg
ctgcttatggcaccggcgggggcagattcaccgtggatttgctatagagaagcccagggatgagccgttacagggaaaagctctgtattgtgtaacagcc
acaacaaaggctccggtatccagtggaagatgtctctcccaagctcattggctgctcccagcaggaagatgatgatgatgaacagggggaagcctttgac
tttgatagtgaaggggaagaagaggattctgggaccatcccaaaggcagaagtccaactgagaacgtcctccatcagcattgagagaccccccttagccc
atacaggggaagggacagaaaatctccagaacaagacagaggcagcggctcccgtgtccctctccaggtcctccagctccgtcagtaatggggaccccgt
gggcaggaagaaactcgagatttatacctgcagcctgaagaggagctctgtgaaacgtgattcttcatctgagcaggtgtgtggcccccgcggaactcag
gacggagccatttatgaggacgttccatgtgagcaggagaatcaggatgtgggagccagtacagtaactccctgtgcctccgtagggctgggataccacc
tgatctatgagaacgtgcaccacgagggttccgagcgggacctagaggacacgggttggagttctagtgaatttgagagttacagtgaagactcggagga
ggaggaggaggaggagagaagaaagataaagccagccaaaccattcgttgccttccagcctaagatgacgcagctaatgaaggcggctcggagcggcaca
aaagacggcttggagaagactcgcatagcagtgatgcgcaaagtcactttcctacagcgcaaggaaactacaggcgactcggaggaggaggaaagtggct
tttttgaggtcattgggttggacttggacctgaagcctccaccgtcagagctcggaccaatgcccgaaggactaagtgctcagcaggtcctgcggcgcca
cattctggcatccattgtgcaaagtgagcggagctacgtggagtccttacagcgcgttctgcaggattatcgcaacccccttatggacatggaacccaaa
ctgctcagtactcgcaagtgccagatggttttctcccgagtaaaggagattctgcactgccattccgtcttccagatcgcgctctcctcgcgggtggccg
agtgggacgtctccgagaaagtcggggatctctttgtggcttcgttttccaagtccatcgtgctggatgtgtacagcgagtacgtcaataacttcaccaa
cgccatgacggtcattaagaaggcctgtctcactaagcccgccttcctggacttcctaaggaagaaacaagcggccagcccggacagggtcactctctat
gggttgatggttaaaccaatccagcgcttcccgcagttcatcctcctcctacaggatatgttgaagaacacgccctgcgggcacccagaccgcttgtccc
tgcagctggcacttacggagctggagaccctggcagagaagctgaatgagcagaagcgggtcgccgaccaaatggccgaaatccagcagctttgcaagag
catcagtgaccgcagctctctctataagagtctgagctccgggcagcaccaacttcttctctgtgagacactgacggagatggtttatggggacaaaggg
caactcctcaaatccaaagagcggcgcctcttcctaatgaacgatgtgctcgtctgcgccaacattaacttcaaggggcagcctgatattgggaatttag
tgccgctcggccctcggtacgtcctgaagtggaacactccgttagcacagatctatgtggcagagctggggcaggaaggcaacgcctttgagaaagattt
ggcggcagggcaacaaggggtgggcagaaagcattcgttctcaagtcagttacaaaataaagtatccctgggctccccgcgcctcttccaggagctgcag
gatgtcaagcgggatctggaggtcgtggagcagataacgcagctaatcagcaccctccatggcacctatcagaacctgaacatgacggtggcacaggatt
ggtgcctggggctgcagaggttggtccggctgaaggaagaagagatccagtctgctaacaagtgccatctccagctgcagcttcccagaaaggcagacaa
gtctgggcggccaatgagtttcatggttgttttcatcactccgaacccactgagcaaaatttcctgggtgaaccggttgcacttggccaagatcgcactg
agggaagagaaccagcccggttggttgtgcccggaggaggagaagaaggtgaaagctccgttttggtctccgacgctggtctgccgcctacccgcattct
ccaccaaagctgccgacctgcagctgggaggcgcggtgcacacccccgtacagaactcgctcttgggcttctcctccgtcagcacctcgttacctcaggg
gtatttatgggttggcggcgggcaggtcggaggggcaggacacatacagattttctccctcaatcacacatccccacgcatgctgaaggcttttcccctg
ccatcttgtgtgctgtgtgtggagaacgtgtgcccgagagcgcccgaagggaacgacgagagggacgaagaagagagtgcggctggctctgcgcgtcccg
ctgtgtgcgtcgggctgcaggacgggagcatcatggtgtatgggaatgtggacactggcaccccctgtttaatggcggtggggagcccgggggtgcagcc
tgtaaagtgcctgaggcacagcccaagcttcctgtgtgcgggactacaagatggctctgtggcgctttacccagccacccaaggaggactgtgggacccc
gggagcccattggtcgtaaaggtgggagatggcccagttcaggccttactggtaatcgatggctgcgtgtgggcaagttgccgaaatagggtgaccgtgt
tggagccagggaccctaaaaactgagaccttcgaggccgtacaggatgagggtatcagcattacccacatggtgaggtcggggggaggggtctggatggc
attttccggaggttcctccatccgactcttccatacggagacgctggaactgctacaggagatcaacatagcaacccgcaccatgcttctgccaggacaa
aagaacccgcgcatcacggccctactcatatgccaggggttgctgtgggtgggcaccgatctgggcatcctggtcacgctgcctgtgccaaagcttgagg
ggattcccaaaatcacaggaaaaggaatggtttccctgaatggccactgtgggccggtccagttcctcacgttggcctgcagttccattgcccccgatga
gcttaggactgatcaaggttctggggaggacatcagccaaagcgacgggccggcagaaccgaaggaggatacaccagatgggccggatctcccctctgac
aacgccccgccttcccgcctgaaggggatcctcctgcagtaccggctgaggggcacctcccacctcccggggctgcacctttcggccagggaaggttcca
gaactgatagctgcaccccggcaaccagcgaggaggacggttctatctacgagatggcggatgagccggatgtttggtgccggagccggccctgcagtaa
ggacgctaaccgcaaggagatcacctcggtggttatagtgtcagggggggcggggcatcgtaccttccacccctccccctgcgcctcccagcctgggggc
gacacagacagtaccctgctgatgtggcagttgcccctggtcctatagcccccgccctcgctatggtcggactctagtacatacggctgcgacccggtcc
ctttagctggtcacttagatactcaggaatgttcataatgtggcgccccctgtccctgggggggaaaatatcaactgtttctatttaaactaaatataag
tcatatttataactagcaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa
5' EST: CX828918 [+]cacgttttttgctcccagcactttgattggctgtacagcagtggattctagatatgagacctgctctctgtatttgaggatgatgttctgccctctccta
gcacctgcagagttctgaacttttggtctgaaaaaaatataacacttctaagcatctcttactgtatttactgagctcttaagcattatagcacagtagc
ccttgccagggatgttgtcttcttatttactaactttcaaagacactgaaagcattacaagatatttctcacattcaaagtaaaggttaaagaacagagg
agtttcatggcttctctgagaagagtcaaaatcttgttggtgttaaacctgattgcagtagctggatttgttctatttttggccaagtgcagaccaatta
ccgtgaaaggtggggaatcattccatgaggtccatcccagggcagaagtgaccaactttacaaatcatgcaagcagccctattcaagaagccgtgctgaa
aagactcgctcttctagaggacatagtctacaggcagctaaatggtttatccaaatcacttggtcttattgaaggttatgggggcagaggaaaaggtggg
gtttcagctacactctctccagatgaagttgagaaagccaaaggaccacatgaaaagtatggctacaatgtctacctcagtgacaagatttcactggaca
ggtctataccagattatagacccactaagtgcaagacactgacctatcccaggagctgcctcagatttcaattattttcatctttgtcaatgaagccctg
tctgtgattctgagatcagtgcacagtgctgtgaaccacacacccggtcatctgttgaaggagattatccttgtggatgacaacagtgatga
3' EST: CX828917 [+]tttttttttttttttaatagttggtatctttattgtttttatgagaccactgaaagtcttcaatcactaaaataagatactttatctaccagtagttttt
agaacattttcagatttgggttttttttataatggtcagttcctgttcctgtcaagtcatcagccattacatagagctctgaagcatacggaattcatag
aagcaaacaaaatgaagcaaatatattttcttaagtgatcaataacaaaacatcaaaaaagaaatcataacaagtatgttcacctaatctgcacttggga
aaatagtctgtttcaaaataatatataaatggtgcagaaataaataatctgtaaatactgtacagagatcattaaaagccttgttctcgatgttatgggc
ccattaaatgatgccacatatgtgttttcctcccttgtatttccaggaataagaatattgttatgtcttttgaatctgaacagcagccaagaagcaaaag
acagattatgaaaaatgttccacaaaccaaaatgctttatggcactgtagtcatccatagcctgtgtgtgtatgtttgtgtgtgtatgtgtaggggtgtg
tgtgtatgtgaaggggtgtgtgtaggggtgtgtgtgtgtgtatatgtgtaggggtgtgtttgtgtaaatgtataggggtgtgtgtgtgtgtatgtgtagg
ggtgtgtgtgtgtgtaggggtgtgattgtgtaaatgtgtaggggtgtgtgtgtgtgtatgtgtagggacggtgtgtgtttatgtgtaggggtgtgtgtgt
attatgtgtaggggtgtgtgtatgtgtaagggtgtgtgtgtgt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: