XB-CLONE-3307028
Clone Name: IMAGE:7657939
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Gene:
fgf2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136120 [+]
GTGAGAGGTTGTTGTTTGTAGTTGCCCTCAGCAGCAAGAGGGGCCGCACAGCAGGGGAGGAGGGATAGTCCTGTATAAATAAATAAGTCCTGATGGCAGA
GGGCTGGATCAGAATGAGGAGCAGACTGCACGCTGCCTACACTCACAGCCGCCTCACTTTTCTCATATAAAAAGGCAGCGGGACTTCAGCAGCAGCCATC
AGCAAGGGGCAGGCACAGAGATGCTACGGAACCCTTTGATTCACAGGAGATGACAGACTCGCTGAGGGCTGCAGAAGGGAGCCTTTTCTGATGCTGCCGG
GTCACTCTAGGAGCGCTGCCTGTAAAATACCTCACTGAGGGAAAAGTTTGCCAGCTTTGCAGTTGCAATTGAATGAACTGTGAGGCCCTGAGCAGGGAAA
ACTGAGGCGACACACAGGGATTTTATCAGATACCACACTGCTACCTGCTAAACCTCGTCTAGGAGAGGCTTCTGGGAAGGCAGGGATGGCAGCAGGAAGC
ATCACAACCCTACCAACCGAATCTGAGGATGGAAACACTCCTTTCCCACCGGGGAACTTTAAGGACCCCAAGAGGCTCTACTGCAAGAATGGGGGCTACT
TCCTCAGGATTAACTCAGACGGGAGAGTGGACGGATCAAGGGATAAAAGTGACTTACACATAAAATTACAGCTACAAGCAGTAGAGCGGGGAGTGGTATC
AATAAAGGGAATCACTGCAAATCGCTACCTTGCCATGAAGGAAGATGGGAGATTAACATCGCTGAAGTGTATAACAGATGAATGCTTCTTTTATGAACGA
TTGGAAGCTAATAACTACAACACTTACCGGTCTCGGAAAAACAACAGCTGGTATGTGGCACTAAAGCGAACTGGGCAGTATAAAAATGGATCGACCACTG
GACCAGGACAAAAAGCTATTTTGTTTCTCCCAATGTCAGCAAAAAGCTGATCTGTGATGACTCCCTTTGTAGGTCACCCTTTCTAAGACCAGAAAATACT
TTTATATTTGTAATATTAAATGTGAGGCTGAGGTACAGCATTGGTATATTGTATATTTGTGCAGGCTCTTGAGCCAGTTTTTGAGAATGATGAATCCAAA
ATACAATAGTCCACTCAGTATTTAATCTACATGTAATATATTGAATATTATACTTGCATTTCTAAATGAAGTGTCTGGACAATTTAGATGAAAATTCTTT
AAAATAGCATAAAATTAGAGCATCATTTGTAGTAAAAGGAGAATTAAAATGTGAAGCTTATAAGTGGTTGGCACTTCCTTCATAGGTCTCTGGTAGTTCC
TTATGGTAAAGTGAATAGAAAAGCCCTGAGTAAACAGCTGGGAATGTTCTCATAGTTAGGTCCACCAGATGGAAGCCTTCGTTATAACCATAGAGTAGAA
GCTTAGCAACTCTGCTTGTTATGATGGTTGTATTACTACAATAAGCAAGCTCTCGTAGATCCATCCATTCACACTTCAAGCATTCCTGATGGCAGAAGTT
TATAGTGTGTGACAATGGCTTCAGGCGACAAATGATGTACAAGTCAGACTTTCCAAATGCCCCAGGGTGATTATGCTGCTGCCTTATGCTTAATAAGGAC
TTAAATTCCGAATGAATGCCAGTCTCTTCAAAAACTTCTCTAACTGCTGTAGCTCCTACAATTGAGAGAAAAAAAACATTTGCTTTAAGTACAGCAACAG
CTTTCCAAAAAACCTTTGCTTTCAAAACAGAATACAGACTGCTTAGTATGCAATTTGTACCAATACATTCTTAGTTCTACTTTTAAGAGGGATTTTTAAA
GTGAAAATGATATTACTACACGGCTGTGTAGTACACAAATAGCAAATATTTCATGGTAACAACTATATTTAATAAAGGGCTGTTCAGCTGGAGGGCTGTG
TGCTTGACATGGCCCTGTAGGGGATTTTTATGACCCCCCAGTGTATTTTATAAATTTGATCATTATAGTGTAATTTGGTGATCAACATGTGTTTTGAGAA
ATAATCGGATCATTACATATAAATAAGTTAAACAGCACTGATATTAGATGACAAGAAACCCCCCTCTGCCATTTTTGAAACAGAAGGGGTTATTATTTAG
TCTTACTGTATGCTCCCATACAGGTCTTTTTCCTTCCTCCACAGAATCATGAAACACTTACATGTCCTCCATACCTGGCCATAAAATGAAGAGCCTGGGT
ACTGGATGTGAAGCAGCTAGACATGGTGGCTGGCCACCTTATTTTACTAAATTCTAAGGCCAGGTACAGAGAAGGCGGCAAACTTTGAGGGAGCCCTTGT
TGCGGTTTGTTGGAGGAAACGGGGACCTGTGCAAGGGGCACTTTTTCAAAAGCAGTATGTAGGGTTTCCTTGTCCTTTTTATAAACTGAAAAAAGTAAAA
CCCATTCTGCAATTTCACTTGGTACTGAACATCTTTATGTTGACAGATTTAAGAAGTTGCAGAAATCTACAGGAACTGCCAAACAGAATTGGTTATTTAT
AATATAAATTAGAATTACTGTTATAAGGAAGGTCATGCACTATTCATCTAGGCTCTCAGTGCTATTGGATGAATAAATATATATTATTATCAGCTATAAA
TCCTTAATGAACCAAACACCAAAATTAAATGTTGGATAACAAGATTATTGTTATATTTAAATGAAAAAGTATGAACAAATCTATAACACTTACCTATATC
TTCCCCTTGATCTGACAGACCACCAGGAAATTTCCATGCATTTACTGTCTGTAATAAAAAAACACAAAAAAACCTGTCACTTCTCTGTATTAAACTTTTT
AAAAAAAATCTCTCTTAACATATATTAATATATAAAAAAAATCTACATTTCTTTCTACATTCTTGCAATATAAGTTCTCACTTTCTACACATCTGCTTCC
TTGTCCTCTTAAATGCAGAGCTACAGGCAGTCCTTAAAGAACATTAAAAAAGAGAACCTCCCTATATTATATACACATTTAACAGCTATGTTGCTCATCC
TGTAGTTTGGAAGGCAATTTTGGGTGCATGAGCAGCTGTGAATACATAGCTTGCAAATCAGTGTAGGACTTGTACAAAGCACTGGCTTGACGGGGTATGT
TGGTTTATGCATGTATGTAAAAACAATGCGAGCAAATGGGTCTTGTTTTTAACTGAATGCAGGTATTTACCTTTGCTGCCTGTAGTGTGGGTAATTCACT
TTTTTCCTGAAAATCAATTTATACCAATTCCTACTCATGAACTGCTAAGAAAATATTGAAAAGTATTTAATTTCGCCATGTCTCATGTACCCGGGATTGC
CTTTTGGACTACGGGATGAATGAGCAACATTGCTGTTGAATGTATGCAACACAGTGTGGATTAGTGTGGCCATAGAGCGCTGTAAAGAGGGCAGCAGGAA
GAAATTGTAAGGAAATTGTAAAATATGTGCAAAACATGGTGTACATGTACTATATCCTCAAGGCCAAATTAAGCCATTTGAAAAAGACTAAGTTATCTAT
TGAATAATGCTAATGGAACCCTCTGGCTAAAAAAATCTACTGTATAAAAAGAGGTTCAGACAGCCAACGCTCCCCAGTATGGCCCTATGGCTTAACAAAA
GGTAATGCCACGTGTTTGCTTGCATCAGTTGTAAAACAAAACATTCAGCACAACTTATCAAGAATAGACTCATAGGAATTGCTGAGTTACACTGCAGTGT
GGATTACTGTGGAAACAGAGAATGTACAAGTATAAATGTCAACCTTTACCTTTTACTGTAAAGTCAATGTGTCACATACTGAAGCATACTTAATAAACTC
TCATCTGGTACTGATGTTATATTTCAACAATGCTGCTAGCCATAGAGATACCTGTAACTTGCAGAAGCTGCTATCTGAAGTTAGTAATGGGACTTTTATA
AGATTTATTTTATCACCAAGACATTTTGCAATAATTGTATTTAATCCTCTACATAGATTTTAATGTGTAGTGTGTTCTTTTGGGCACTGAAATCAGTGAT
GTGTTTATATTTGTACTTACAAAAAATGTATTTCATGCAATAAATATAATAAAATTCATTTTAATACAAAAAAAAAAAAAAA
5' EST: CX830006 [+]tgagaggttgttgtttgtagttgccctcagcagcaagaggggccgcacagcaggggaggagggatagtcctgtataaataaataagtcctgatggcagag
ggctggatcagaatgaggagcagactgcacgctgcctacactcacagccgcctcacttttctcatataaaaaggcagcgggacttcagcagcagccatca
gcaaggggcaggcacagagatgctacggaaccctttgattcacaggagatgacagactcgctgagggctgcagaagggagccttttctgatgctgccggg
tcactctaggagcgctgcctgtaaaatacctcactgagggaaaagtttgccagctttgcagttgcaattgaatgaactgtgaggccctgagcagggaaaa
ctgaggcgacacacagggattttatcagataccacactgctacctgctaaacctcgtctaggagaggcttctgggaaggcagggatggcagcaggaagca
tcacaaccctaccaaccgaatctgaggatggaaacactcctttcccaccggggaactttaaggaccccaagaggctctactgcaagaatgggggctactt
cctcaggattaactcagacgggagagtggacggatcaagggataaaagtgacttacacataaaattacagctacaagcagtagagcggggagtggtatca
ataaagggaatcactgcaaatcgctaccttgccatgaaggaagatgggagattaacatcgctgaagtgtataacagatgaatgcttcttttatgaacgat
tggaagctaataaactacacacttaccggtctcggaaaaacaacagctggtatgtggcactaaagcgaactgggcagtataaaaatggatcgaccactgg
accaggacaaaaagctattttg
3' EST: CX830005 [+]aaaatgaattttattatatttattgcatgaaatacattttttgtaagtacaaatataaacacatcactgatttcagtgcccaaaagaacacactacacat
taaaatctatgtagaggattaaatacaattattgcaaaatgtcttggtgataaaataaatcttataaaagtcccattactaacttcagatagcagcttct
gcaagttacaggtatctctatggctagcagcattgttgaaatataacatcagtaccagatgagagtttattaagtatgcttcagtatgtgacacattgac
tttacagtaaaaggtaaaggttgacatttatacttgtacattctctgtttccacagtaatccacactgcagtgtaactcagcaattcctatgagtctatt
cttgataagttgtgctgaatgttttgttttacaactgatgcaagcaaacacgtggcattaccttttgttaagccatagggccatactggggagcgttggc
tgtctgaacctctttttatacagtagatttttttagccagagggttccattagcattattcaatagataacttagtctttttcaaatggcttaatttggc
cttgaggatatagtacatgtacaccatgttttgcacatattttacaatttccttacaatttcttcctgctgccctctttacagcgctctatggccacact
aatccacactgtgttgcatacattcaacagcaatgttgctcattcatccc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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