XB-CLONE-3305982
Clone Name: IMAGE:7658458
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Gene:
ntrk2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC123936 [+]
GAGCCAACTCTCCATCTCTGTTTGTCTCTATCGGGCAGGAGCTGAAATCCCTGCAGCCCCCCCCAGAAGGAGCAGCTGGGAGAGGGAATCAGCATCCTTT
CTGTGCCGGACCTTCTGGGATCACATCACCAATAAAGCTGCACTGGTACTGAGGAGGCAGAAGAGGTGAGAAGCTTACAGTGAATGAGCCCTTTCTCCTA
CCTTTGCTTGCTCGCCAACGCTGAGCTATGGTGTGTATCACTTAGCGCATTTGGATTATGACTGAACCGACGCCTCGTCACTGACTCCCACAGATTCCCC
GGACCTATTGCTTTTCTGGATCAAACGCCTGGTGTTTAGGGGGATCCCCTCTCCATTCTAGGGGGATCCACTGGACCGGAGATCCACCATGCGCCTCTGG
AAAGGCTCCCACGGACCTGATTTAACTCAGGTGTGCGGCGCTCTATGGATCCTACTTGCCTTGGTTTGGAGGGGCCTGGCTTGCCCCCAGTACTGCAGCT
GCAACTCCACCAGGATTTGGTGCACTTTAGAGGGCAAAGGGATTGTGGCTTTCCCGGTGCTGGAGGACAGCGCACTGGCGGAGAACATTACTGACATTTA
TATTGCAAACCAGCGATCATTGGCAAGTATCAATGATGACGATGTAAAGATTTACACTGGACTGAGAAATCTAACTGTGGTGGACTCAGGTTTACAGATT
GTATCACGGCAAGCATTCAGCAAGAACCCTAAACTTACATACATAAACTTCTCCAAAAATAAGCTGACATCTTTACCCAAGAAGCTTTTTCGTCATCTAA
CTTTATCTCAGCTATTTCTAGGTGGCAATCCTTTCCAGTGCTCGTGCGATTTAATGTGGGTTAAAGTATTATTGGAGACTAATTCCCTAAATCAGAACCA
GAACATATACTGCTTCAATGACAACAAAAAGAAGATACCTCTCTTCACTATGCACATCCCCAACTGTGGGTTGCCTATAGTAAATGTGAGTACATTAAAT
ATCACAGTTCTTGAAGGTAATGAGACAACCTTGTTCTGCGATGCCAATGGACTCCCAGACCCAAATGTTTCCTGGGATATCTCACAGATCATTTCAAAAA
GCTTGAAAGAAACTACAAAGCGTCCAGCCTTGCTTACCCTGAAAAATGTAACATCGATGGACAACAAAAGAGTCATTGTATGTGTGGCAGAAAACAGCGT
GGGTGAAGATCAAGTCTCTGTGGAACTCAATGTACACTTTCCCCCAGTGATCACTTTTATTGACTTGCCAACACTGGACCATCATTGGTGCATACCTTTC
AGTGTGAGGGGTAATCCGAAGCCTACACTGCAATGGTTCCATGAAGGGAGTATGCTCAGTGAAACAGATTTCATTTGGAGCAAGATTCATGAAACAAGTA
ACAATACAAGCGAGCACCATGGCTGTTTGCAACTGGACAGCCCAACACATTTAAACAACGGATTTTATACATTAAGGGCTGAGAACAAATATGGAAGGGA
TGAACGATCCATAAGCGCTCTTTTCATGGAACGTCCAGACGATGGTAATGATCCGATTACTGACCCTGGATTTTATGATTATGAAACCACTTCCAATGAT
ATTGGTGGAACAAGTACAGATATTGGAACAGGCGTCACTTCTACAGATGTTTCTAAAGAAGGAAATGAAGACAGTATTACTGTTTATGTCGTAGTGGGCA
TTGCAGCATTAGTTTGTACCGGCCTGGTAATAATGCTGATCATCCTAAAATTTGGAAGACACTCCAAGTTTGGGCTGAAGGGTCCATCCTCCGTGATAAG
CAATGATGATGATTCAGCCAGTCCCCTCCATCATATTTCCAATGGCAGCAATACCCCATCTTCATCAGAAGGTGGTCCTGATACAGTAATCATTGGCATG
ACCAAAATCCCTGTCATTGAAAATCCACAGTACTTTGGAATTACAAACAGCCATCTTAAATCTGACACATTTGTCCAGCACATAAAGAGACACAACATAG
TCCTGAAGAGAGAGCTGGGAGAAGGAGCCTTTGGCAAAGTTTTTCTAGCAGAGTGTTACAACCTCTACCCTGAGCAAGATAAAATACTGGTTGCAGTAAA
GACATTAAAAGATGCCAGTGACAATGCCCGAAAAGACTTTCATCGGGAAGCAGAGCTGCTCACAAATTTGCAACATGAGCATATTGTGAAGTTTTATGGT
GTTTGTGTGGAAGGTGACCCGCTCATCATGGTCTTTGAATATATGAAGCATGGCGATCTTAACAAATTCCTTAGGGCCCATGGACCAGATGCAGTCCTCA
TGGCAGAAGGGAACCGTCCTGCTGAACTAACTCAGTCCCAGATGCTTCATATAGCACAACAGATTGCTGCAGGAATGGTGTACCTGGCTTCGCAGCACTT
TGTACATCGAGATCTTGCAACGCGGAACTGTCTAGTTGGAGAAAACCTCTTGGTGAAGATTGGAGATTTTGGCATGTCAAGAGATGTGTACAGTACCGAT
TACTACAGGGTTGGTGGGCACACAATGCTGCCAATCCGCTGGATGCCCCCTGAGAGTATCATGTACAGGAAATTCACAACAGAAAGCGATGTTTGGAGTT
TGGGAGTTGTATTATGGGAGATTTTCACTTATGGAAAACAGCCTTGGTATCAGCTTTCAAACAATGAGGTAATAGAGTGCATAACCCAAGGGAGAGTTCT
CCAGCGTCCTCGCACGTGCCCGAAGGAAGTCTATGACCTCATGCTGGGTTGTTGGCAGAGGGAGCCTCATATGAGGCTTAATATCAAGGAAATACACAGC
CTCCTTCAGAACTTGTCAAAAGCATCTCCTGTATACCTTGATATCCTTGGCTAAGAGATCAACGTGTTAGTTGTTCATGCTCGATAAATGAATGTGGTGA
ACTACTGCAGAACTCCATGAAATGTGTTATAACCTCTGACAGTATTATTGACAAAGATTCTGAGAAGCTTTCAAAGGGCAAGCTACATAAACTACTCCAT
TTCTACAATGCACAGTATTGACTATTTGCCATGACTATGTTGTATCTTTTTCTCCCAAGCTTATGTCTACTTTAGCATTGTTCCTACTCGCCTTCAGTTT
TCCTTTGGTTCTCTCTTATATTACCCACCACTGTATGCTTCTGCCCTTACGTAATACAGAATAATTAGACAAAAGGCCTTAAAACCTTCTCTGTTAATCA
GCAGGCAAGCATGTCTGTTTACCACATCTAACAATGCCTTGTATTTCCTGCCTTCGATGTGGATCAAAAAAAAAAAAAAA
5' EST: CX837770 [+]caactctccatctctgtttgtctctatcgggcaggagctgaaatccctgcagccccccccagaaggagcagctgggagagggaatcagcatcctttctgt
gccggaccttctgggatcacatcaccaataaagctgcactggtactgaggaggcagaagaggtgagaagcttacagtgaatgagccctttctcctacctt
tgcttgctcgccaacgctgagctatggtgtgtatcacttagcgcatttggattatgactgaaccgacgcctcgtcactgactcccacagattccccggac
ctattgcttttctggatcaaacgcctggtgtttagggggatcccctctccattctagggggatccactggaccggagatccaccatgcgcctctggaaag
gctcccacggacctgatttaactcaggtgtgcggcgctctatggatcctacttgccttggtttggaggggcctggcttgcccccagtactgcagctgcaa
ctccaccaggatttggtgcactttagagggcaaagggattgtggctttcccggtgctggaggacagcgcactggcggagaacattactgacatttatatt
gcaaaccagcgatcattggcaagtatcaatgatgacgatgtaaagatttacactggactgagaaatctaactgtggtggactcaggtttacagattgtat
cacggcaagcattcagcaagaaccctaaacttacatacataaacttctccaaaaataagctgacatctttactccagaagctttttcgtcatctaacttt
atctcagctatttctaggtggcaatccctttcagtgctcgtgcgatttaatgtgg
3' EST: CX837769 [+]tttttttttttttttgatccacatcgaaggcaggaaatacaaggcattgttagatgtggtaaacagacatgcttgcctgctgattaacagagaaggtttt
aaggccttttgtctaattattctgtattacgtaagggcagaagcatacagtggtgggtaatataagagagaaccaaaggaaaactgaaggcgagtaggaa
caatgctaaagtagacataagcttgggagaaaaagatacaacatagtcatggcaaatagtcaatactgtgcattgtagaaatggagtagtttatgtagct
tgccctttgaaagcttctcagaatctttgtcaataatactgtcagaggttataacacatttcatggagttctgcagtagttcaccacattcatttatcga
gcatgaacaactaacacgttgatctcttagccaaggatatcaaggtatacaggagatgcttttgacaagttctgaaggaggctgtgtatttccttgatat
taagcctcatatgaggctccctctgccaacaacccagcatgaggtcatagacttccttcgggcacgtgcgaggacgctggagaactctcccttgggttat
gcactctattacctcattgtttgaaagctgataccaaggctgttttccataagtgaaaatctcccataatacaactcccaaactccaaacatcgctttct
gttgtgaatttcctgtacatgatactctcaggnggcatccagcggattggcagcattgtgtgcccaccaaccctgtagtaatcggtactgtacacatctc
ttgacatgccaaaatct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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