XB-CLONE-3305887
Clone Name: IMAGE:7658815
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Gene:
prrt1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127556 [+]
GATCAGAGGAGAAGCTGTGAGGGGGGGAGACAGACAGAGGGGGCGAGGAGAGACCCCCATCCTGCCATATCCATCTGAGGACCCCCCACAAGTCAACGAA
GCTCCCTCCCCCTAGAGATATGTCCAGCGAGAAAACAGGCCCTCCAGACTCTGGACCACACAATTCTCCTCCTCCTTACAACCAGCCCGTTGGGGAAGGC
CCATGTAATGGAGTCATCCCTGGATACAATGACCCCCCTCCTCAGCCAGCCACAGCAACCCTTCCTCGACAGGACCCTCAAGGATACCTTCAGGAGACGC
CATTCACTGCTCCCCCACCAGCTCCTCAAGGGTATGTGGTACAGACTCACCCTCCCGGCGGCACCATACCATCTGGGGGATATATACCACAGGGACCAGG
GTATCCCATGCACCTGCAGCCCTGCACTGCTTATGTACCTGTATACCCTGTGGGGACGTTTCTGCCACATTTCTACCTCCTCCAGCAATATCCAGCAGGA
GCAGTTCCACCCCAGCCTGGGATCCCACCACCTCAGATCCCACCTGGATTGGCATTACTAGAGCCACGACGTCCACCTCATGACTACCTGCCCATTGCTG
TCCTCACCACTATCTGCTGTTTCTGGCCAACTGGAGTTATTGCCATAATAAAGGCTGTGCAGGTCAGAACAGCTTTAGCCCGTGGGGACCTGATGTCTGC
AGAGATAGCTTCCCGAGAGGCACGGAACTTCTCATTCATCAGCTTGGCTGTGGGCATTGCTGCAATGGTCTTGTGCACGATCCTAACAGTGGTTATTATT
ATTGCAGCTCAGCACCATGACAATGAGTGGGGGCCATAAATGACTCCCCATGGGCCAAAAGGAAGACACCTGCCTTTTTCCTCTATATATTTCTCTTCTC
TATCCATTTTAGTTTAAGGCTTACTTTCTTCATTCCCCATCCTCCCTTAGCTATGTCTTCCTATTATTATTTCTTAGTTTCACCATCTCTTTATCTTATT
AATTTCTACTGTGCTATTTCTCTTATTCCTCCTACTGATTCTCATTTAGATATTCTCTTTCAGTCTCTCTTCTACACATTATCTGATTATCTTCTAATTG
TTATAAATAATACAGGTGCCAAATGGGGGTATTATGTATGGGGTTCTAGGAGCATCTGGGGGTACTGTGCAGCTCCACATGAGAGGTTGCAAAGGATTAT
GGGCTGAGGGTGCAATTCAGGGAGACAGAAAAATTGAATGAGACAGAGAATGATGATGTGCCTCTGTAAGAAAGAGAATACAAGATGCTGATTCTCTCAG
GCCAGAAGATTTAGACATGAGCTAGTTGGTCATAGGCACATTGGGATAACATGACAAAAAGACAAAAGAACAAAGTAGTTTTTCAAGAGGAAGAGTGTAA
AAGAGTGTGTGTGTAACAGCATCAACAAGCTAAGGGAACCATGGAATAAAAAGAAGCTTGTTGGACTGACTGCGAAAATAATAGAAAGTATAGCAAAGCA
AAATGTGCTAAGCTATAGTTTAAGATACAAGCTGCTATTTTATGGATACATTTTTGGAACACTGTTTGAAAGAATTAATCTAATAGTCTTTCAAACCAAT
GTGTATATTAGCTATAGCTTTTCTTAAAAATGCCATGTACCTCGCAGGAGAGTACTAGTAAATGTGAGACACTACAATAATAGCAGGACAGACATCCAGG
AAAATGATTTCAGTGCCAAGAGTGGACAGCCTGAGCAAAAGAGCATATAAAATGGAAACATGATTGGCAGTGGAACTGTAAAGTTTGCCTTTAATTAAAC
TTTTAGTATGTAATAAATAGCCTTTACTAAACATCTGGCTTTTCTGTTCTGCATTTTTTGGGCTAATGTCCCACAGAGAGATTGCTTGCCGGCGATAGAT
CTCTGCTGCCACGGGTGACTAATCTCTCTGAAATGCCTTTTCGCCGACGGGGGGGGAGTTGCCGGTGGAGGGGCTTACTCATTGCTTTTGTTTTTCAAAG
TTTCCTCCTGCAGTCAACTTTGCATGATTTTGGAAAACCAAAGCGATACTTGGCCTTTCCACCGGCTGTTCCCTTTGTTGCCGGCGGAAAGGCATTTCGG
CATTTTTGTGGTGGCAGAGATCTCTCGCCGGTGACTTGTCTCTCTGTGGAACATTAGCCTTAAACTGCAACTGAAAATGATATATTGTTGTTGGGAGATT
GATTATGAGGGTTCCATTTTATTGTTGTTTCTTGCTTCTATGTTATGTTTCATTTCAGGCCCTCCTATTCATCTTCCATTATGACTTTGCTAGAGCAATC
AGATAGCAGTCAAAATTCAAATCTAAAAGTTGCAGGACAGAAAAAAAAAAAAAAA
5' EST: CX837925 [+]tcagaggagaagctgtgagggggggagacagacagagggggcgaggagagacccccatcctgccatatccatctgaggaccccccacaagtcaacgaagc
tccctccccctagagatatgtccagcgagaaaacaggccctccagactctggaccacacaattctcctcctccttacaaccagcccgttggggaaggccc
atgtaatggagtcatccctggatacaatgacccccctcctcagccagccacagcaacccttcctcgacaggaccctcaaggataccttcaggagacgcca
ttcactgctcccccaccagctcctcaagggtatgtggtacagactcaccctcccggcggcaccataccatctgggggatatataccacagggaccagggt
atcccatgcacctgcagccctgcactgcttatgtacctgtataccctgtggggacgtttctgccacatttctacctcctccagcaatatccagcaggagc
agttccaccccagcctgggatcccaccacctcagatcccacctggattggcattactagagccacgacgtccacctcatgactacctgcccattgctgtc
ctcaccactatctgctgtttctggccaactggagttattgccataataaaggctgtgcaggtcagaacagctttagcccgtggggacctgatgtctgcag
agatagcttcccgagaggcacggaacttctcattcatcagcttggctgtgggcattgctgncatggtcttgtgcacgatcctaacagtggttattattat
tgcagctcagcaccatgaccatgagtggggggccataaatgactccccatggggcaaaaag
3' EST: CX837924 [+]cctgcaacttttagatttgaattttgactgctatctgattgctctagcaaagtcataatggaagatgaataggagggcctgaaatgaaacataacataga
agcaagaaacaacaataaaatggaaccctcataatcaatctcccaacaacaatatatcattttcagttgcagtttaaggctaatgttccacagagagaca
agtcaccggcgagagatctctgccaccacaaaaatgccgaaatgcctttccgccggcaacaaagggaacagccggtggaaaggccaagtatcgctttggt
tttccaaaatcatgcaaagttgactgcaggaggaaactttgaaaaacaaaagcaatgagtaagcccctccaccggcaactccccccccgtcggcgaaaag
gcatttcagagagattagtcacccgtggcagcagagatctatcgccggcaagcaatctctctgtgggacattagcccaaaaaatgcagaacagaaaagcc
agatgtttagtaaaggctatttattacatactaaaagtttaattaaaggcaaactttacagttccactgccaatcatgtttccattttatatgctctttt
gctcaggctgtccactcttggcactgaaatcattttcctggatgtctgtcctgctattattgtagtgtctcacatttactagtactctcctgcgaggtac
atggcatttttaagaaaagctatagctaatatacacattggtttgaaagacta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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