XB-CLONE-3305877
Clone Name: IMAGE:7659009
|
Gene:
kif6
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC154107 [+]
GGAAAGTGTCAGGTACCGGGAACATGGTGAAACAGGCGATTCGTATCTTTGCGAGGGTGAAACCCAGCAGAAAACCGGCCGGGATCTATTCCGTTAGTAA
TGAGGAAGAAAGAGCCTCTTCCCTGGAGATCCTCGTTCCCCGAGACCTTGCCGACGGCTTTGTCAACAACAAGCGAGAAAGCTACAAGTTCAGATTTCAA
GAAACGTTCGACCAAGATGTGAAACAAGACACGATATTCGAGAGCATCGCCAAACCCGTGGCCGAGAGTGTTCTTACAGGTTACAATGGCACCATATTCG
CATACGGGCAGACAGGCAGCGGCAAGACCTTCACCATCACGGGAGGCGCCGAGCGTTACAGCGACAGAGGAATCATCCCTCGCACCCTGTCCTACATGTT
TGAGCAGTTCCAGAAGGACAGCAGCAAAGTATATACGGCACATGTGTCCTACCTGGAGATCTACAATGAGTGCGGCTACGACCTGCTGGATCCGAGGCAC
GAAGCGTCCAAACTGGAAGACCTTCCGAAAGTGACCATCATGGAAGATCCTGACCAGAACATTCACCTGAAGAACCTTTCCCTTTTGCAAGCAAGCAACG
AGGAAGAGGCACTAAACCTTCTCTTTCTGGGGGACACAAACAGGATGATTGCAGAGACCCCTATGAACCAAGCCTCCACCCGCTCCCACTGCATCTTCAC
CATTCACGTTTCCAGCAAGGAGCCCGGATCCGCCACCGTACGACGTTCCAAGCTCCATCTGGTGGATTTAGCCGGGTCGGAGCGAGTTGCCAAGACCGGA
GTCGGAGGACAGTTACTTACAGAAGCCAAATATATCAACCTGTCACTTCATTACCTGGAGCAGGTCATTATAGCTCTGGCAGAAAAGAACCGCTCCCATA
TACCCTATCGGAACTCCATGATGACTTCTGTGCTCCGAGACAGCCTGGGGGGCAACTGCATGACCACCATGATTGCAACTCTGTCACTGGACAAGCGAAA
CATCGATGAATCCATTTCTACGTGCCGCTTTGCGCAAAGAGTTGCCTTAATTAAGAACGAGGCCATCCTTAACGAGGAGACTGACCCCCAGCTGATAATT
GTTCGACTGAGAAAGGAGATTCAGGGGCTGAAGGGGGAGTTGGCCCTGCTGACAGGGGAGCAACGGACTGAAGAACTGACCGAGGATGAAACTCTGAGAC
TGGAGGAACAAGTGCGGGGATTCCTTGAAGACCGAGACCCAGAAAGCAGCCTGGATATGGGGGCCGACATGCGCAAGATTCAGTACTGCTTTGCCTTCAT
AAAGAAGCTTCTGCATGGAAAGAAGGAAGAGGAGAGCAGAGCTTCATCTCCAGTGAATGCGGCTGAGAGACCCAGCAGTTCTGCCCCACGACCCGAGGAG
ATCAAGAAGCTCCGAGACATTCTGCAGCAGAGAGACAATGAAATAAATATCCTGGTTAGCATGCTGAAGAAGGAGAAGAAGAAGACTCAAGACGCAATGT
TTGAAATGGCGCCTTCTAGGCTTAATGCCCCCACGCGACACTCAGACAGCTCAGCGCAGTCCGGCAGTTTGGACCAAAGTCCCAAGTCGTCTGTATCTGC
GGCTGTGGAAGATCAGATGGGATCTCAGGAATGGAGGTCACATTCTTTTCCCAGGAAAACAGGAGAGGAAATGTCTCTCGGACGGCAAGAAGCATTTGAA
ATCTTCAAAAGGGATTATCCCGACCGAGTCACCATTGAGGATAACAAGAGGCTCCTGAAGCAGAGGTTTGCTGAAGCAAAGTCTCTGGGAGAAGAAGTGA
ATAAAGTGAGAAGCAGAATAAATCAGATGAAAGGGGATGTGGAACAACGAAGGATCCAAAGAGCAGCGCTGGGCATCTTCAGCTCCTCTGATGAACAGAA
CCAGTACGACCCAGTGGAGGAAGAACTACGGGAGCAAATGGAGGAAGAGAAGAAACGATACAAAGGGCTTTTTAGTCGCCTCAAGAACCTGAAGACGGAG
ATCGAACACCTACAGCTTCTCCTGGAGAAGGCGAAAGTGAAGCTGCAGAAAGACTTCGAGGGCTGGTGGGTGGAAGAGGCGGCACATCTGCAGGCTAAAC
AGGAGGTGCACAAGGCAAGAATGGCCACATCTGCCCCACCCTCCCCACAGGTGCACGAGCAGAGCAGGGCTCCTCGGATGTTGGACACCCCCATCCCGCT
GACTGGGGACAGCCAGACCGACGCAGACATCCTGGCCTTTGTCAGGGCCCGACAGAACCTCCTGCAAAGAAGAGGGAAGCCATGATGCCCACTGAGCCTC
AGACCCGCAGAGCTCCGGCCAATCAAGCAGTGCCAGCATGATCATGTGCCCATTATGGGGAAACCCTCACATGAACAGGAAGTGACCTCACATAGAATGT
GCCGCCTTTATCCTCAGGGGTCCCGGCCCCCCAAAACTGGATTTTCAAGCAGAACCAGATGTGATTGCAGTGAGCTGCTTTCCTATATACATAACAATTG
TGCAGCTCCTGGGTATTACACGCACACTGCCTGTATATGTGTATATACCTGTATATATGGGGCCCTAATGGGCATCGACTGACTGAGATATTATTATAAT
CCATGTGCTGTTATTACTGTTTATATGATATAAGAATCTGTCGTTTGTGACGTCACAATGTAATCATGCAGTTGTGACATCACTCATGTACTGTACAGGG
GGTCATATTACCCACAGTTAGGGTATAACTGGCGGGTACATAAGCAAAGCCTGACAGGCAATAGTCCCTAAAGGCAGAGTTAATAAAATATTGTTTTCCC
AAAAAAAAAAAAAAA
5' EST: CX837946 [+]gaaagtgtcaggtaccgggaacatggtgaaacaggcgattcgtatctttgcgagggtgaaacccagcagaaaaccggccgggatctattccgttagtaat
gaggaagaaagagcctcttccctggagatcctcgttccccgagaccttgccgacggctttgtcaacaacaagcgagaaagctacaagttcagatttcaag
aaacgttcgaccaagatgtgaaacaagacacgatattcgagagcatcgccaaacccgtggccgagagtgttcttacaggttacaatggcaccatattcgc
atacgggcagacaggcagcggcaagaccttcaccatcacgggaggcgccgagcgttacagcgacagaggaatcatccctcgcaccctgtcctacatgttt
gagcagttccagaaggacagcagcaaagtatatacggcacatgtgtcctacctggagatctacaatgagtgcggctacgacctgctggatccgaggcacg
aagcgtccaaactggaagaccttccgaaagtgaccatcatggaagatcctgaccagaacattcacctgaagaacctttcccttttgcaagcaagcaacga
ggaagaggcactaaaccttctctttctgggggacacaaacaggatgattgcagagacccctatgaaccaagcctccacccgctcccactgcatcttcacc
attcacgtttccagcaaggagcccggatccgccaccgtacgacgttccaagctccatctggtggatttagccgggtcggagcgagttgccaagaccggag
tcggaggacagttacttacagaaagccaatatatcaacctgtcacttcattacctggagcaggtcattatagctctggca
3' EST: CX837945 [+]tttttttttttttttgggaaaacaatattttattaactctgcctttagggactattgcctgtcaggctttgcttatgtacccgccagttataccctaact
gtgggtaatatgaccccctgtacagtacatgagtgatgtcacaactgcatgattacattgtgacgtcacaaacgacagattcttatatcatataaacagt
aataacagcacatggattataataatatctcagtcagtcgatgcccattagggccccatatatacaggtatatacacatatacaggcagtgtgcgtgtaa
tacccaggagctgcacaattgttatgtatataggaaagcagctcactgcaatcacatctggttctgcttgaaaatccagttttggggggccgggacccct
gaggataaaggcggcacattctatgtgaggtcacttcctgttcatgtgagggtttccccataatgggcacatgatcatgctggcactgcttgattggccg
gagctctgcgggtctgaggctcagtgggcatcatggcttccctcttctttgcaggaggttctgtcgggccctgacaaaggccaggatgtctgcgtcggtc
tggctgtccccagtcagcgggatgggggtgtccaacatccgaggagccctgctctgctcgtgcacctgtggggagggtggggcagatgtggccattcttg
ccttgtgcacctcctgtttagcctgcagatgtgccgcctcttccacccaccagccctcgaagtctttctgcagcttcactttcgccttctccaggagaag
ctgtaggtgttcgatctccgtcttcaggttcttgaggcgact
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: