XB-CLONE-3305712
Clone Name: IMAGE:7658947
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Gene:
egr2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136205 [+]
CTGCTCTGCCAGGGACACTACAACTGCACTGGCACTCTCACTGCTGGAAGCAATGCCAACATGTGCCTGCTAAGCCTACCCTTGGGATGCAGCTAGAGAC
GCGCCTCAGAGCTGCCAGGGACATCTAAAGAGCTGTTTGTTCCCCCACTATAACAATAATAATAATAATTCAGATTTGCTTGAATTGACCCTGGGGGCAC
TCGGTGAGACCATGGCTGCCAAAGCGGTAGATAAACTCCCAGTGACTTTTGGTAGTTTTGTGCATCAGTTCCCAGAAGGCATCTACCCCGGGGAGGATTC
CACCCTGCCTGCCTCTGTGACCATCTTCCCTAATGTGGACCTGGGGGGACCCTTTGATCAGATGAACAGTGTGACAGGAGATGGAATGATCAGTGTGGAT
ATGAATAATGAGAAGCGATCGCTGGATTTCTCCTACTCGTCCAACTACCCCTCTGCCCCCCGCAGCCAGCCCTTAGCCTATATGGGCAAGTTCTCCTTTG
ACCCCCAGTACCCCGGGGCCGGCTGGAATACAGAGGGAATCTTCAATATAGTGAGTGCTGCTAGCATCCTGGGGGTGCCCCCCTCCTCATCTTCCTCCAC
CACCTCCTCTAACGCCTCATCGGGGTCCCCCAACCTGAGTTGCAGCATGGCTCACCCCCAGAGTGACATGGACCATATCTACTCACCTCCCCCCTACTCC
AGCTGCAATGAGATCTACCAGGACCCCTCGGCTTTCATTGGCACCTCCACAGGGGCACCCTACCCGCCCCCTTCCTACCCTTCTCCTAAGGGGGCGTCGG
ATGGGAGCGTCTTCCCCATGATCCCGGACTACTCGGCTCTCTTCCCTCCCCAATGCCAAAGAGACTTGCACTCCATGCCCGATAGGAAACCCTTTCCGTG
CCCCCTGGACTCAATCCGGGTCCCCCCACCTCTCACCCCCCTCTCCACCATCCGCAATTTCACATTGGGGGGCTCCAGCAATGAGGGGTCCAGGCTGCCC
AGCGCTTACAGCCCCCATAACTTACCACTTCGACCCATCCTCAGACCCAGAAAATACCCCAACCGCCCCAGTAAGACCCCTGTCCACGAAAGACCTTATC
CCTGCCCGGCAGAGGGCTGCGACAGACGCTTCTCGCGTTCTGACGAACTGACCAGACATATCCGAATTCACACTGGCCACAAACCCTTCCAGTGTCGCAT
CTGTATGAGAAACTTCAGCCGCAGCGACCACTTGACTACTCACATCCGGACGCACACCGGGGAGAAACCTTTCGCCTGCGACTACTGCGGGAGAAAGTTC
GCCCGGAGCGATGAGCGCAAAAGGCACACAAAAATCCATCTGCGGCAGAAGGAGAGGAAAAACTCGGCAACAGCGGCACCTAGCGGCAGTGCGACCCAAG
AGCGACCGCTGAGCCTGAGTCCCGCAGGGACCGGTAACAGTATGTGCTCCACTGGGTCAAGCAGCGGCCAATTGGGACTGTGCCCTAGCCGGACAGGGTG
ACACACGGACAGTGTAACCCCCACACAGGGATCAAGGGGTCCGTCACTCCCACAAGGATTCCCCAAGAAATCGCACAGCCAGACTTAGTGGCAGTTATCT
TTATCCAGGCAATCACCTAGGATACAAACTGGAAACAACCTCCAGCATTAACACCTTTTGATGGGGGGGGTCTATGGTGGGCAAGTTACTGTCAAACGCT
AAATAAATGCTTGGTGTTTAGTTCTGGCAGCAAAGTCGAGGTGTGTAAGTCAGAAGGGAGTTAGTGACAAGTCAGTGTAATGTCACCAATAGGAGCTTAT
ATATATGCCAGGGAATGCTTGGGCACAAGTGCTGGGCATCTGTACCATTCATGCAGGGGCTGAGTTGTGGGTTATTAAGGATAAATTCTCTTAGCCCTGC
AGAGTTTGCTTAACTACAGCTCCCATCATTTATCTATTGGGAAAAGCAGAGAAAAAGCTGAGCCCCTGTGCAGCCTCTGCAGTAGCAGCTACAGTCCAGC
TAATGGCTGCACAAAGGACACATGGGAAACATTTAATTACAAATTTGCAGCAGGGCAGTAATCCAGCAACTAGCTGCAAATAACCCAGGGTTACAATAAT
GGGTTGCTATTGGTTACTACCTTGGTGCAATTTTGCAGCTGGTCTAGTAACTGAAGGCCAAAAATAATTTCCAGTGAGACATTGTGGTACTGTCCCTTTC
CCTTTCTCTCCAAAGCCCCTATGAAAGGAGAATAGCTACATTATTTTATTGGTTCTCCTACTGCAAAAAGACAGTCACATGATAAATCTCTGCCGAAACA
GTGCAAAGTCTTGGCAGGAATTTCTCATAGTCAATCCAATCACATTATATTGCACCATTTAATATAAGCAAGCCATTAAAGTGACAGTGGCTGAATTTTG
GGAGCAAACACAGTATATTAGAGTGTCGCTGTCTCATCCTTCAGCAGAAAAGGGGGTGTCTGATAGTCCTGGGTGGCCTAATAGACCTGGGCGGGTTGAG
CTCCACAGAGTCAAGTGCTGAATAATCAGGTTTGCCTTTTATTTCTTACTAACCGTGTGTCATTTTCATGCGTGATTCTTACTTTTTTGTAACTTTTTGG
GGGCTAAATTCAGGGAAAAATGGAGATATGGTAACTTTGAAAGGTGTTTTCTATCCTTTCCCATCCTTGGGGTGAAGCACCTGTAGTAGCCATAGTTTTG
TGCACTTTATAACTTCATGACTGCTCCGGAGAGGCGGCATGATATGAAGGGAGGATGTCTGGAATTTCTAAAGCCTACCCTAGATTTAATATTTTCATTT
TTTTCCTAATTAATAACATTATTTTTTTTTTGGTGTAACCAATACAGTGTGACTAACAGGAGTGCATTCTAAAGGTACTGTTACGTTTGGCACTGCTGAG
TTGTGTAGAACCCTTACATCTCTTTAGGGGACTATTTTATTTCTTTTCAGTGTAAGCCCTGCTGCTGTTGATGAACTACAGATCCCAGAATCCTTAGCTG
GGCCTTGTAGTTCAACAACGTGGCAGCAGACATCCACCGACTGTCAATAATGTTGATCTGCACTGTTTTACAGAGTTCCAGACCTTTTTGGTTGGAGTGA
AACAGGACTCTGTATGTAATATAGAAGCGCCAGAGAGCCTAATTTATTTAAGGATGTATATAGTTTGTGTACGACGTTAGATTGCATGCCTCTCTGCTAC
TCAGTGTTCATGGTGCCTTGTGTGATGTGGACAATTCTCGCCCTGTCTGCATGTATATATAGGTTGCCTTATCCACGACACAGCGGTGGCCCGGGGGCCA
CCGTTTAACATAGCTGTACATTACTGCCAATTAATATTTGGAATTTTTTCTAGAATTTCTATTTTTGTACCCAAACGCACTGTTTTGTGACCCAAAAGTG
TTAACTTTGTAGTTGAGTTTACAAAAAAATACAAAATACGGGATAAAAAAATAAGGAAAAAAAAAAGAATATGGCATTGACGGGGCTGCTTCTGTACTCG
GAAATAAAGTGTTAAAGAGTTTACTTTATTAATAACCAGCCACTAACTGGAATGGAATCATGAAATGTACTTAGATACAAAGACTGTAAATAAATACTTT
TCAACTATAAAAAAAAAAAAAAA
5' EST: CX838197 [+]ctgctctgccagggacactacaactgcactggcactctcactgctggaagcaatgccaacatgtgcctgctaagcctacccttgggatgcagctagagac
gcgcctcagagctgccagggacatctaaagagctgtttgttcccccactataacaataataataataattcagatttgcttgaattgaccctgggggcac
tcggtgagaccatggctgccaaagcggtagataaactcccagtgacttttggtagttttgtgcatcagttcccagaaggcatctaccccggggaggattc
caccctgcctgcctctgtgaccatcttccctaatgtggacctggggggaccctttgatcagatgaacagtgtgacaggagatggaatgatcagtgtggat
atgaataatgagaagcgatcgctggatttctcctactcgtccaactacccctctgccccccgcagccagcccttagcctatatgggcaagttctcctttg
acccccagtaccccggggccggctggaatacagagggaatcttcaatatagtgagtgctgctagcatcctgggggtgcccccctcctcatcttcctccac
cacctcctctaacgcctcatcggggtcccccaacctgagttgcagcatggctcacccccagagtgacatggaccatatctactcacctcccccctactcc
agctgcaatgagatctaccaggacccctcggctttcattggcacctccacaggggcaccctacccgcccccttcctacccttctcctaaggnggcgtcgg
atgggagcgtcttccccatgatcccggactactncgctctctttcctccccaatgccaaagagacttgcactccatgcccg
3' EST: CX838196 [+]tttttttttttttttatagttgaaaagtatttatttacagtctttgtatctaagtacatttcatgattccattccagttagtggctggttattaataaag
taaactctttaacactttatttccgagtacagaagcagccccgtcaatgccatattcttttttttttccttatttttttatcccgtattttgtatttttt
tgtaaactcaactacaaagttaacacttttgggtcacaaaacagtgcgtttgggtacaaaaatagaaattctagaaaaaattccaaatattaattggcag
taatgtacagctatgttaaacggtggcccccgggccaccgctgtgtcgtggataaggcaacctatatatacatgcagacagggcgagaattgtccacatc
acacaaggcaccatgaacactgagtagcagagaggcatgcaatctaacgtcgtacacaaactatatacatccttaaataaattaggctctctggcgcttc
tatattacatacagagtcctgtttcactccaaccaaaaaggtctggaactctgtaaaacagtgcagatcaacattattgacagtcggtggatgtctgctg
ccacgttgttgaactacaaggcccagctaaggattctgggatctgtagttcatcaacagcagcagggcttacactgaaaagaaataaaatagtcccctaa
agagatgtaagggttctacacaactcagcagtgccaaacgtaacagtacctttagaatgcactcctgttagtcacactgtattggttacaccaaaaaaaa
aataatgttattaattaggaaaaaaatgaaaatattaaatctagggtaggctttagaaattcc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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