XB-CLONE-3305550
Clone Name: IMAGE:7658836
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Gene:
numa1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC127551 [+]
CGGCAGCCAATGGCGGACAGGGTCTTACATGTAGTCTGAGACTTGAGGAAAAGTCGTGAAACAATATGGCGGCGCACAGCGGCAAGATGGAAGCATTGCT
CTGCTGGGTGAACAGCCTGAAGGTGGGGGAACCAATCGAGAGATTTTCACAGCTGCAGGATCTGGGCATTCTGCTGAAAGTTGTCGGCATATTATCAGGG
ACATCCGAGGAAACGGCCCTTGTCCTACAGCAGAGCCCGGAGGAGCGGCTGAACTTCCTCTGTGCCTTCTTACAAAGGTATTGCCGGCCGGGCTCCTCAG
CTGAAAACTTGGTGCAATGGCAGAAGATTCTGCAGGGCGAGAACCTGGAAGTGGAACTTTCCAAGGCAATCGTCCTTCTCTTCTATTACTCAACTATGAG
CTCCAAAAACCCGAAGAAATCTGAGGATTTTGATCATAAAACTCAGACAGAGCTGGCCTCCATCTTGCGCTTTGTACTGGATAATGAAGATGCTCTCTGT
CTGGACGACAAACTTATCAACTTCCTGCAGCGGAAAGCGCGGTTTCCCTCCTTGGGAGATGATACGTCCAGTTGCTCCGACGCCGCGTCTCCTAATGTGT
CCCACAGGCGGAAGAGCGAAGTGCGCTTTCTGGAGCTGCATCGTGTTGCTTCCTCTTCTACTATGAAAAGTTTGTCAGCGGACCCCCCATCTTCCCCAAT
GATCGAGGTGCTGCACACCCCTCAGTTCCAGATGAGGCGCCTGAAGAAGCAACTGAGTGAAGTGCGGGACTGTAGAGATGAACTGGAGGTGGAACTGGCA
GAGAACCGCAAACACCTGGCAGAGAAAGAGGCTCAGATCTCCCTCCTGCAGCAGCAGATTGAACACCTTCGGATCTTGAGGGAGAACCAGGCTGAGCTAC
AGGAACCCAAAGAGCTGGAGGAACTTCGGGAGAAGAATGAGAGCCTGATGATCAGACTTCGGGACACCCTCAAGCAGTGTCAGGATATGAAGACTGACAA
GAAACAACTGGAACGAAAGAACGATCAATTGGCCGAGGAGAATGGCGAGCTCTCTTACAAGGTGCGAGACTTATCCAACCGCTTGGCACAACTGCAGGAG
GCTCTCTATGAAACCACAGAGGAACACGAGCTTTCTCTGAGCAACTGGCAGCAGAAACAGAACCAGTTAGAGAGTGAGCTGAGTGGTGCAGTCGGTGAAA
AGAAGTATCTGGAAGAGCATCATTTAATACTGCAGGGAAAAATATCAATGCTTGAGGATCAGCTAAAGAAGATGGGGGAGAGAGAGGTGGAAGAAAAAGG
AGATTGTATGGGTGACATCCTCAAGCTGGAAGACTTGAAGCAAGAGGTTACTGCGCTCAGCACACAATGCCTCAGTCTAACAGAACAGATCCATCAGTTG
GAAGAGGAAAAGAGCACTACAGAAATTGACATGGAGGCACAGAGATCCAGATTTGAATCTGAGAAAAGTCAGCTGCAGGAGATAGTCACTAACCTGCAGA
CTTCATTGTCAGAAATAACATTTCAGAAGGAGAGGCAAGATAACGAGGCCAGGGCACAACAGGAACATCTGACGTGCCAGATTACAACATTAAAACTGGA
GATCTCCAAGCTGAAGTCTTCCCTGGTGCATAAGGAGGAAGAGCTGAAGGGCATTCACCATCAAGTGGAAGAAGAGAAGAGAGAAAAAAATCAGCTCTTG
GAAAACTTCAAAATGCTAGAAGAATCCTCAAAGCAGAACATTCAGGAGCTTGTAAACCAAGTGGAGCAAATTAGTAGCTCTTTGAAGGTTTCCGAGGGCA
ATCTGGTTGGACTTACACAACAGCTAGAGAGCAAGGTGAAAGAAGTTGTCTGTTTAAGAGAAGAACAGCAGAAAGTTGTCTGTGAGAGAGATTCCACTCT
TTCCACATTCAATGAGTACAAGTGCAAAAAGGATGAAGAGTTCAGTGTCTTAAACATGACCATGAAAACACTGGAACAAGATCATCAAGCTAGTCTTTCT
GTTATAGAGGAGCTGAAAAGGGAAAAGGCAGAACTGGCATCCAAGCTACAGGAACTGGATGCCACCATTCTAGATCTTCTTGCTAAATGCCAGAACTTGG
ATAGTGAAAATGCTTCACAGAGCAAATCTCATGCAGCCGCTGTGGAGTCTCTAAAGGCACAGCTTTCAGAGCAGGAGAGTCAACTGAAAATATATCAGAA
GAAGGTGTCCTCCTGTGAATTGGTGTCCGAAGAAAACTCGCAACTTAAAGAACAGCTCCTGTCTATGGACGAAAGTCTGAGACATCTTAGGGAACATCTA
GAGAAAGAGAAAATGAAATTTGCTGCTTCTCTTGATAGCGACGGTAAAAGAATTTCCCACCTTGAAGAGGAGATGAAGAAGTTGAGTGAAAGCAGAGATG
CAGCACTGAGTAACCTTGCTGAAGAAAGGGCCTCCGGACAGAAGATTGAATCTCAGCTGAAGCACTTACAGGAGGAGTACCATACAGCAAATGAAACCCT
GCAGGCAAAAATGGCAGAATCCAGCGCTGCTATCAAACAGCGTGAAGCCGAAAGGGACGAGTTGTCTAAGGTGGTTGACATCTGGAAGGCCAAATATGAA
GAGTCTCAGCAGAAGATAGTGCAGAACTCCTGCCAGATGGAGGAACAAATTAAACATCTGAAGAAGGCTCATACTGATGTTTGCCAGCAACTGGAGGGAG
AGAAGAGTAAAGTTTTGATGTTGGAAGCCCAGGCCAAGGAAACAAACTCTTCTCAACTGGAGCAGATAAATCGGTTAGAGTGTGAACTGTCTGAAGCCTA
CTCTCGTATCAAAGAGAGAGAAGCTGAGGAAAAGAAGCTACTCTCTGCTCTCCACTCTTCAGAGGAGAAATTTCAGATTGCTCATCAAGGGGAAAGTGAG
CGTCTTTCCCATCTAGAAACAGCCTTAAGCACTGCAAAGTCAGAACTGGATTGTCTAGTCAGAGAGGTGTCTGATGAGAAGCATAAGAAAGAAGAACTTG
AAACCTTGGTGAAAGAGTTAAAAGAGCAAAAATCTGAAAGAATTCAGTCCCTGGAATCTGAGGTGAAAACCAGTTTAGCTGCTGTTAAAGAGCGAGAAAG
TGAAACTGCAAAGCTTTCTGCAGAGGTGAAGGATCTGAACAGGCAGTTAGAGGAAATCGGTCAGAAGCATAAGGAAGCGCTGGCTCAGAAAAATACTGAA
ATTGAGCAGTTGATTGAGGCCAGAGAAAAAGCCGCGTTGGACTTGGCTAGTAAAACAGAGATTGGGGCTCAGCTGCAGAAATTGTTGGATACGCACAAAG
GTGAGCTCAGTGCCTTGCAGAATGAGCTTTCCCGTTCCCTTGATCTCATCTCTCAGAAGGAAAGTGAAGTAGAAAGACTTCATAAAGAGGCTGCTGTAAC
ACAAGAGGAGATACGGAAGCAGCAACAGACTATGGGAAAGCTCACCGAAGAGCTAACTGCTTTGGTTGCACTTAAAGAACAGGCGGCCCTGCAGGAAAAA
GAGATTGCACAGCATGTCCGGGCCGCTAAGGGTGCTGAAGCAGAGATGGCTAACCTTAAATCTGTCATTTCTGAAAAAACTAAAAATATTGAATCCTTGG
AACACGACATCAAGAACCAGAAGGGAGACCTTGCTTGCATTCAAGAGCAGTATCGGAGCAAGCAGGAGGAGGCCCAAGGTCTTCAAGGCCAAATAGTAGA
TCTGGAAAGAGAGTGCAAAGAGCAGAAAGAACTGATTTGTCAGGCCCAGAAACAGGCTGCTGAGGCAGAAACGCTGGCCTCCGAAAAGGTTTCAACGTCG
AAGAGACAGCTAGAAGGAATCCAAGCGCTCGAAGGTGAAATCCATAAAGAGAGGCAAAAGACGTGTGACCTGCTGAAGCAGCTGGAGGCTTCTCAGGCCG
CACAGGCAGAAAAGGAAACGGAGCTACAAGCACTGAAGAAAGAGTTGTTTCATAAGGTTCAAGAACTGGAGCAAAGTCAGAAATCTTCTACTGAGTCAAG
TCGAGAGCTCTCCTCTATGCTTTCTGCGGCTCAGGAGAGGCAGCGAGCCTTAACAGAGGCCAAACAACAAGCAGAACAGTACCAGAAGAAAAAAAAAAAA
AAA
5' EST: CX838430 [+]cggcagccaatgttggacagggtcttacatgtagtctgagacttgaggaaaagtcgtgaaacaatatggcggcgcacagcggcaagatggaagcattgct
ctgctgggtgaacagcctgaaggtgggggaaccaatcgagagattttcacagctgcaggatctgggcattctgctgaaagttgtcggcatattatcaggg
acatccgaggaaacggcccttgtcctacagcagagcccggaggagcggctgaacttcctctgtgccttcttacaaaggtattgccggccgggctcctcag
ctgaaaacttggtgcaatggcagaagattctgcagggcgagaacctggaagtggaactttccaaggcaatcgtccttctcttctattactcaactatgag
ctccaaaaacccgaagaaatctgaggattttgatcataaaactcagacagagctggcctccatcttgcgctttgtactggataatgaagatgctctctgt
ctggacgacaaacttatcaacttcctgcagcggaaagcgcggtttccctccttgggagatgatacgtccagttgctccgacgccgcgtctcctaatgtgt
cccacaggcggaagagcgaagtgcgctttctggagctgcatcgtgttgcttcctcttctactatgaaaagtttgtcagcggaccccccatcttccccaat
gatcgaggtgctgcacacccctcagttccagatgaggcgcctgaagaagcaactgagtgaagtgcgggactgtagagatgaactggaggtggaactggca
gagaaccgcaaacacctggc
3' EST: CX838429 [+]ctggtactgttctgcttgttgtttggcctctgttaaggctcgctgcctctcctgagccgcagaaagcatagaggagagctctcgacttgactcagtagaa
gatttctgactttgctccagttcttgaaccttatgaaacaactctttcttcagtgcttgtagctccgtttccttttctgcctgtgcggcctgagaagcct
ccagctgcttcagcaggtcacacgtcttttgcctctctttatggatttcaccttcgagcgcttggattccttctagctgtctcttcgacgttgaaacctt
ttcggaggccagcgtttctgcctcagcagcctgtttctgggcctgacaaatcagttctttctgctctttgcactctctttccagatctactatttggcct
tgaagaccttgggcctcctcctgcttgctccgatactgctcttgaatgcaagcaaggtctcccttctggttcttgatgtcgtgttccaaggattcaatat
ttttagttttttcagaaatgacagatttaaggttagccatctctgcttcagcacccttagcggcccggacatgctgtgcaatctctttttcctgcagggc
cgcctgttctttaagtgcaaccaaagcagttagctcttcggtgagctttcccatagtctgttgctgcttccgtatctcctcttgtgttacagcagcctct
ttatgaagtctttctacttcactttccttctgagagatgagatcaagggaac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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