XB-CLONE-3305497
Clone Name: IMAGE:7659040
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Gene:
chd2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX838527 [+]
caggcgcagggcagctaaaaatgtcagctacaaggaagatgatgactttgagacagattcggatgatctgattgaaatggtgggcgaggaagcagaggaa
ctggaagatagtcatgaaacaatagagaaagttttggactccagaattggaaaaaagggagccattggagcttccactactgtgtatgcaacagaagaaa
atggggatcctaactctgaatttaaccctgaaactgatgaaagggagcaacaatatcttattaagtggaaaggctggtcttacatccacggtacctggga
gagtgaagattctcttcagcagcagaaggtgaaaggcctgaaaaagcttgagaacttcaagaaaaaggaggaagaaatcaagcaatggttatcaaaagtt
tcacctgaggatgtagaatacttcagctgccagcaagaacttgcatccgagctgaacaagcagtatcagatagttgagagggtcatagctgtaaaaacaa
gcaagtcgtcttctgggcactcagattttccagttcacagcaggaagacttcctcctccaatgaaccggagtatctgtgcaagtggatgggtctcccata
ttctgaatgcagctgggaagatggtgcccttgttgggaagaagttccagcactgtattgacagttttaactgcagaaacaactcanaaacaacacctgta
aaagactgcaaggttttgaggcagagaccaaggtttgtcaccttaaaaaagcagccttcttacatagggggtgaaggacttgaactgcgtgactaccagc
tagagggtttgaactggctggctcactcttggtgncagaataacagtgtgattcttgctgatgagatgggcttgggaaagaccatcccaaacaattcatt
tctctcctatctgt
3' EST: CX838526 [+]tttttttttttttttctgggtcagctgggatggatttactgagaacttcaaagtcttcctcgtgctgaataatagcttttacattcacttgcacaccaga
gattttaattgtgggtcccctgcgcttgcctggaccttttccatcagcagggttttcttttaattgctcttcgaattcctgcatggctgaagcacagctg
ttgtgcagcagttcacccaacctcttgagatctgccacagatttttccacaagctctgcatctcgggcaatgcactccagtctttccagtggagaagcaa
acttcttgtaagccttaataaaccgtctgatctcagcatcagtaaatccctcaacattgtctttcctaacacttcgtggccgtcctctgcgctttggctt
tttgtcatcatcggagtcatctgagtcactctcggatccagaggaacgctgagctttctgctttgactccaaatcagactcactgtcatttgcttgaggc
ttcttgttcaagctcctgatcctgggaagcatgtatatttcctgtagttctttctgccgagcctcctcctccactttttttctttgcccctcaggaatta
tgtcatcccattcctgttcgcgcttcaaaaccatttcaggctcttcctccattgttgcaaagttagctaccttgaactgagacagcaactcatcagtagc
actggaagatggctcgttctctcgagtttccgcaagacgtaaaatttcgtctatatccatttcctgaggttctaattcctctccttcagtttccttgaaa
agatcagcagctccaaactttagaatagcagcaagttcatctttgttaaatgggttacaattggagct
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: