XB-CLONE-3304948
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Clone Name: IMAGE:7826233
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Gene:
h3-3b
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Species: Xenopus tropicalis
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Sequence:
5' EST: DN098299 
[+]
aagcaatgggctcgtacaaagcaaactgcccgtaaatccactggtggaaaggctccaaggaagcagctggctacaaaggctgccaggaaaagtgctcctt
ccacaggtggtgtgaagaagccacacagatacaggcctggcactgttgccttgagagaaatcagaagatatcagaagtctactgagctgttgatccgaaa
actaccattccagcgacttgtaagggagattgcccaagacttcaagactgatttgaggttccagagtgcagctattggtgctcttcaggaagcaagtgag
gcttacctggttggcctctttgaagataccaacctgtgtgccattcatgccaagagagtaacaattatgcccaaggacatccagttagcaagaagaatcc
gtggcgagcgtgcttaaacattttgctaggaacttttttttgaacttagtttttttttttacaattgtacatacaagtgtgatgtcttttattttataaa
gggtttgttaactgtagagtagttgacagtaagatggtagtaaacattttatatgacattcccttaatcctcaggtttttcagaaatttgtatctgcagc
tagtcttttgtgaccctctcctttaaaacatagtgcatgcccatacttcattctttacataatttagtatctttctgtgtaactccattgtaaataaact
ttccactacctcaaaaganaaaaaaaaaaaaaaaa
3' EST: DN098298 [+]cttttgaggtagtggaaagtttatttacaatggagttacacagaaagatactaaattatgtaaagaatgaagtatgggcatgcactatgttttaaaggag
agggtcacaaaagactagctgcagatacaaatttctgaaaaacctgaggattaagggaatgtcatataaaatgtttactaccatcttactgtcaactact
ctacagttaacaaaccctttataaaataaaagacatcacacttgtatgtacaattgtaaaaaaaaaaactaagttcaaaaaaaagttcctagcaaaatgt
ttaagcacgctcgccacggattcttcttgctaactggatgtccttgggcataattgttactctcttggcatgaatggcacacaggttggtatcttcaaag
aggccaaccaggtaagcctcacttgcttcctgaagagcaccaatagctgcactctggaacctcaaatcagtcttgaagtcttgggcaatctcccttacaa
gtcgctggaatggtagttttcggatcaacagctcagtagacttctgatatcttctgatttctctcaaggcaacagtgccaggcctgtatctgtgtggctt
cttcacaccacctgtggaaggagcacttttcctggcagcctttgtagccagctgcttccttggagcctttccaccagtggatttacgggcagtttgcttt
gtacgagccattgctt
Library:
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5'- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3') and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1408 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine).| Anatomy: ovary | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI |  |
Usage:
Suppliers:
