XB-CLONE-3301604
Clone Name: IMAGE:7757732
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Gene:
spon2
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Species: Xenopus tropicalis
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Sequence:
5' EST: DR875985 [+]
atcaagtgacatcaacaaaatacaagtcaacatttgaaattacctctgtcaactcttttgatgaaggcagctacacagtagtagttgagaattctgaagg
aaagcaagaagcacattttactttgacaattaaacgtggaaaggcagcagaaaagggtgttacatccccatctagagtaaaatctccagagccacacaaa
aaatcccatgagtctataaagtcaccaaaaagagtgaaatcccctgaaccaactacaggttcaaaagctaaaccatctagcaagtcgcctgcagataaag
tacagctacccacagttgcagctccaaagattgctcagaatctaaaagttgcagcaagtgatgataaagttaaaatgtcctgcatagttgaaagcacctt
actaagtggaagagaagtgacatggttaaaagatggtaaaaaactgaaagaagactctcatttcagtttccactattcagctgatgggacctacgaactt
acaatacatagtctttcacagactgaccagggtgaatatgtttgtgaaattactggagaaggtggtgtgtccagaactacactgatgttcacagggcaaa
cctttaaaagtgtcttttcccaagtgtccagtgcaattgagtcaaagaggataattcagaaatctaaatccgaagtttcatctcaatcaaaaatgattat
ggaggaaaccagcaagactgaactggtcgctgaagaaatcagagctactcaggctgaaattaaagctttagtaagttctgtncaattcagtgaaggccaa
aaagtcacactgaaagccaatatacctggagcatctcanat
3' EST: DR875984 [+]aggtttttttaatggtgcagaaaacatgctttattgcaatcaaagagctatcttcgggaggttcaataaataataatgctacaataacataaaataaaac
aataaataatgacgaagtgaagggcattcggaagttcttaacaacagcctgaaaagctgcggtgcatcactttattgccctgttttctcaacatataata
tagtccctattctaccacacacaatcactgcacccgataaacctcctgaagttggcactgattctgcacttgagtaccttaacctgctttgttgagggta
tggccaggtgatgttactttaaagcaagctgagggagtcaatgtgtcagaaaaagtgcagagggttttttatgaacaaagtgctggccgcttgcactttg
aacatacaaaagttttgtctctgtaaaattgtaaatttccattatctgtaaataatgtaaatatgtaggtgttgcctgcaggagggtagttgcaagcagc
acctaatattcacagtaattcaatccaaatacttggatttcgtatagttcgtgtagagcatcctctcttgggtgtaggtttgtcttcaacagggagtttc
tcaacctgctgaggtttgcttagacatcaacgtagagttgaggcacatagtttaatggcaggcaaggtattcgctccctaggaaactttctcctgcccac
cgttggttccagtttcattttaaaacgtcataaaatatatggtacagaactccataaccggtaaactcggctatatacagtatgtacatgtcagttaaaa
atgtatcaat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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