XB-CLONE-3301438
Clone Name: IMAGE:7659262
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Gene:
st6galnac4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121364 [+]
GTAATAGCTGCGTGTGTATGGAGGGCCGGAACGTTTCCTGATCTGGCACACAAAGGCCGACCTTTGGCCTGTTAACTTACAAGGACATATGCCAGGGCTC
CCCTTTCTAAAGCTGGTTTTAGCCAGTATCCAAGGAGTCAGCTGAGTGTGAATAGCAGGACCGTATGTGATGGTGCTAAGTGATGATACCGGCCCATGAA
ACATGAGAATCATAGCCCGGTTGTGCTTGGGAAGCCTGTTGCTATGTGCTGTTCTTTGGCTTTGCTGGATCAAAGTAAACACCAAGGAAAAAGCTGATGA
GCAGCCGAGAACTTTACCTCAAATTCTGACATTGCCAGGATATAGCCGCCTCCCAGATGGACAGCCCCTCTCCAGGAACCCCTGTAAATCATGTGCTGTG
GTGTCCAGTTCAGGGCAGATGCTGGGATCAGGCCTTGGGCCTCAGATTGATCAAGCCGAATGTGTCCTACGCATGAATACAGCTCCAACTCTGGGTTATG
AGGCGGACGTGGGCAGCCGGAGCTCTCTGAGGGTGGTATCCCACACCAGTGTCCCTCTTCTGCTGCGCAATCAGACCTACTTCTTTCAGCAGTCGCAGGA
CACTGTATATGTTATCTGGGGTCCCCTTAGGCAAATGGATATCGACAGTGGCATCACGTATCGAGTTCTGCAACATACTAAAGGGATGTACCCCCAGGTA
CAGATCTACACACTGACACAGAGCATGATGGCACACTGTGACCTGGTCTTCCAGAATGAGACAGGGAAGAACAGGGCAATGTCTGGAACATTTCTTAGCA
CTGGTTGGTTCACCATGATTCTGGCCATGGAGCTGTGTGAAGAGATCACGGTGTATGGAATGGTTGGCGAAAATTACTGTAGAAATCGGTTTAATCCCCC
TGTGCCTTATCACTACTATGAAAAGGGGAGGCTGGACGAGTGTACTATGTACATGATGCACGAGAGAGCTTCAAGAGGAGCCCACCGCTTCATCACAGAG
AAAGCTGTATTTGCTCGCTGGGCCCACAGAAAGCAGATCCATTTTGCCCACCCCTCATGGGGCGCTTGAAGAGGATGTGGATGTCTCCTTTTTTCACCTG
TTCATAGTTACAAACCTGGAGCTGTTACCTCCAGCAATGCATTGGTCAATGCTTCTTCTCTATTGGAGTGCTGTATGTCCAATACCGTACACAGACGTCA
AGAACTGACAATCTAATCCGTGAATACTAGAGAACTGAGAAGAAACTTTAGCAGAGATCCCTGACTCAATGGTTTGAAAGCCTTTATAAATACAGGGCAC
TCCCACTTTAGAGAGCAAGCTGCTACATCATTAACAAGTAATGGGTGGCCCCTATAGATGTTGCTGAAGCTTGGCAACACAGAGAGGCTGCCAGTGGCCC
ATTCGCTGTGCTATATTATATTCTCTTTACAGGTTGAATGTACTGTGGTAAAATCGATGTTGGCGTATGAGGTGTAAAGTACAATGGGGATCCTGAATAT
TGTATGTACTAGCAACAAGTGACACAAATGCTGCCCTCAGCCTGACAGGCTCCTTTCTCCTTTTAGTGCCAAGATAAACTGGCACCTTAGTGCCAAGGAT
AAGATAGTCTAGACCTCTATGGCAACTCTGTTGTAAACCACTACTGCTGCCCCTTTAATCACCATATTACTTCCTTGAATAGAATCTGAGCCTGAAAAGT
TGCAGACACCAACAACCAAAATAAGTTAGGGCAGAAATGGTTGGAGCCATATGAGGAGGCAATGTACATTGTCTTAGATAACAGGCTTTAAAAAGGTGAC
CTGAGTTGCCTGTATACACACACACACACTGATTAGCCCAGTCCAATAATGAATAGAAACAGTTACCTATTTATATATAATATAATAATTATACCAGAGT
GGGAAGGTGCATTTACCAGTATTATAAAATCACATAGTCACAATATTAAGAGAGAGCTTTCCTTTCCTGCAAAGCCACTTCGCACTGACCATCTCTACCT
AATTTTGGTGGAGCTAATTCCAGCCTTTGTTTACTGTTAGCAACATCAATGCAAAATGGTTATTTTAGGTTTTTTTTTTGTAACTTTCAATTATTTATTA
TTGAGAATCAGCAAGGACTGAGCCCTCTGCGATTCGATGTCTTCTCTTGTCTTGCTGGCCTCCATGCTCCTGCGGCCAGAGATGAGCCAGACACAATGCT
CTTTAATAATGGACTTCCTTGTCAAATAGAATCATGGTACAGGTACAAAAGATATTAATGAGGTCTTTATCTACTTTGTTTGTTTTTAATTGGCTTTTAA
ATTGTATGTGTACTTCTTTATAAACTCGGAGCTTGTAATTTGTTACCTATGCTTTCTCATTTGTAGACTCCCTGCATGCTCTTATGGTCGCTGACTAGGG
GAGCATGCTGGGAGTTTTGCAACAGCAGGATGTGAAATATGCCCTTACAAAGTGGCTATTGAGAAGATGATGATAAGGCATAATACATCTGCCCTGTTTT
TACAATTCCTGCCACCACCTCCCACTGATGTAAATGGGCTACTGTCTTACAATGCAAAAATAAACATATCCCATGCTAACGAAAAAAAAAAAAAAA
5' EST: CX839092 [+]taatagctgcgtgtgtatggagggccggaacgtttcctgatctggcacacaaaggccgacctttggcctgttaacttacaaggacatatgccagggctcc
cctttctaaagctggttttagccagtatccaaggagtcagctgagtgtgaatagcaggaccgtatgtgatggtgctaagtgatgataccggcccatgaaa
catgagaatcatagcccggttgtgcttgggaagcctgttgctatgtgctgttctttggctttgctggatcaaagtaaacaccaaggaaaaagctgatgag
cagccgagaactttacctcaaattctgacattgccaggatatagccgcctcccagatggacagcccctctccaggaacccctgtaaatcatgtgctgtgg
tgtccagttcagggcagatgctgggatcaggccttgggcctcagattgatcaagccgaatgtgtcctacgcatgaatacagctccaactctgggttatga
ggcggacgtgggcagccggagctctctgagggtggtatcccacaccagtgtccctcttctgctgcgcaatcagacctacttctttcagcagtcgcaggac
actgtatatgttatctggggtccccttaggcaaatggatatcgacagtggcatcacgtatcgagttctgcaacatactaaagggatgtacccccaggtac
agatctacacactgacacagagcatgatggcacactgtgacctggtcttccagaatgagacagggaa
3' EST: CX839091 [+]agcatgggatatgtttatttttgcattgtaagacagtagcccatttacatcagtgggaggtggtggcaggaattgtaaaaacagggcagatgtattatgc
cttatcatcatcttctcaatagccactttgtaagggcatatttcacatcctgctgttgcaaaactcccagcatgctcccctagtcagcgaccataagagc
atgcagggagtctacaaatgagaaagcataggtaacaaattacaagctccgagtttataaagaagtacacatacaatttaaaagccaattaaaaacaaac
aaagtagataaagacctcattaatatcttttgtacctgtaccatgattctatttgacaaggaagtccattattaaagagcattgtgtctggctcatctct
ggccgcaggagcatggaggccagcaagacaagagaagacatcgaatcgcagagggctcagtccttgctgattctcaataataaataattgaaagttacaa
aaaaaaaacctaaaataaccattttgcattgatgttgctaacagtaaacaaaggctggaattagctccaccaaaattaggtagagatggtcagtgcgaag
tggctttgcaggaaaggaaagctctctcttaatattgtgactatgtgattttataatactggtaaatgcaccttcccactctggtataattattatatta
tatataaataggtaactgtttctattcattattggactgggctaatcagtgtgtgtgtgtgtatacaggcaactcaggtcacctttttaaagcctgttat
ctaagacaatgtacattgcctcctcatatggctccaaccatttctgccctaacttattttg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: