XB-CLONE-3300943
Sequence:
Full length sequence: BC166166 [+]
gggcattcacacagcgcgaggggcaatagaatttcctacaggttttctgcacaattgctacgttctaattcgtcataatgcccaagtgtatcgttaatgg
atgcccacacaggagtggccaaaaactgctttacccggatgttgttttacatccctttccgaaaaatatcgaaaggattagaaactggctgatgcaaaca
ggccagcactcggaagacctcgaacaacttacccaaaggatatttcaagggtggaaaacggcaaacttccgaatgtgttctaaacattttacggagggtt
cctacatgcaaagcggatcgcgaagaattttaaaaccaaacgcagtgccaacaattttcgatactgtctatgttccagttacaactgtaatggcagtcgc
cccaataggagcttctgctaaacgaagaagggttgacgatggacggccatcgacttcttcgacaatcgtaagagtcgtttcgcgtcttgctactgttgga
acgcagaccgacgtcaaagtgagtatggtcgatacttccaccgtgacatgcaattgctctacacaaacaaaaagaattattactaaggaagtgagttctc
caagtggagaagatttcatccagtcagatgttcatcaaaatcgccatatcctgtgtgttttttaattccttccaaaccaaccagcaaaattcaaaataaa
atcaaagtgatctgcaatcaacgatgcattcaacaccagcaccaatggagtccttaatagttgaggtagaaaaaaatttggatggcagtaggttgagtgt
tcgggcaacatgtttagatcataattcactgctatggaactcacagcccacctttggtaaaacttcaacaggaaagtttttactgaacaatagtggtact
attgaagtgtgtgctttatagtattggagctttattctcctttgattccaggggcacttccaggccagtcccttccacaaggagccaatgggaatgtggg
aggaagcgagtgacccagtgatggggaagaaggataaaaatgaggagcggaaggagagaatcctgaatctcacactggagattatctatctgctgactgg
agagggctacgtcatccctaagaagaagagcccaactgtgggtttgggggccctgcatgcccctggctccgtcatacagaaggaaaatgacaagaagatc
ctggaactcatctccaacatcatccagctgctgactggagaggttgccataaggtgtgaggatgtttccatctatttttccttggaggagtgggagtata
taaaaggaaaccaggccctttacagggaagggataaaggaggaggaggagccccagcagctccgcccactggcagcctgtgagtataaagatgggagcga
tgttacagcacatacggaagcaactttatgttgtaataatgatgggaatctcacaaaccctcatgtttctccagcggaacagcccccaccggccaatggg
attaaagaggaggcggcttcatgtgaagggggaaaccaatcagattacagcattaatccccttacagaacagatacagggaacagacacacctactccta
tcatggggagcagcctgaataacagcttggcagaggattatatatcggttgttattaaagaggaagaatcttcatgggaagggggaaaccaatcagattg
cagcattaatccccttacagaacagatacagggaacaaatactcctatcatggggtgcagcctgaatagtttaaaaatgacgggtaataatcatgatgaa
aattcccaacaatctcacataacagaaaatcacaaacacaataaaccctattcttgttcttattgtggaaaatgttttttaaatcaatccgaccttaaat
gccatgaagtaactcacacaggagagaaacctttttgttgttctgaatgtgggaaatgtttttcaggtaaatcacaccttatcattcatcatagagccca
cacaggagacaaacctttttcttgtaaatgtgggaaacgtttttcaaatcaatccaaccttgctcgtcatgaaaggactcacacagaggagaaaccattt
tcttgttctgaatgtgggaaatgttttttaaaccatttctatcttgcgcgtcatcaaaggactcacacaggggagaaaccattctcttgttctgaatgtg
ggaaatgtttttcagtcccatatcaccgtgatcgtcatcagagaactcacacaggggacaaaccattctattgttctgaatgtgggaaatgtttttctaa
ccaatatcaccgtgatcgtcatcagagaactcacacaggagagaaaccattctattgttctgaatgtgggaaatcttttacaagtcgatctaaccttgat
cgtcatcatagaacccacacagaggagaaaccattttcttgttcttaatgtgggaaatgtttttcaaagcactcttgccttgatcggcatcaaaagactc
acacaggggagaaaccattttctgaatgagggaaattatttgcattgaacagtaatctaaaaatactctttcgcatgagaaaaactgtactttacacatg
aggcatcaggcaaatatttttatgtccaccatgaaatgtttctttaagagaacccttagaccaactctttgcagctaaagactcctgatggacactgggt
aatgctataaagggtctctttacattaaatcagttttgggaaaagccctttttcatgagacgtgtcagactgatctatttgtgcagttaaaaaaatgttt
ttcatgcttgttaataaaacattgaatataaaaaaaaaaaaaaa
5' EST: CX839924 [+]ggcattcacacagcgcgaggggcaatagaatttcctacaggttttctgcacaattgctacgttctaattcgtcataatgcccaagtgtatcgttaatgga
tgcccacacaggagtggccaaaaactgctttacccggatgttgttttacatccctttccgaaaaatatcgaaaggattagaaactggctgatgcaaacag
gccagcactcggaagacctcgaacaacttacccaaaggatatttcaagggtggaaaacggcaaacttccgaatgtgttctaaacattttacggagggttc
ctacatgcaaagcggatcgcgaagaattttaaaaccaaacgcagtgccaacaattttcgatactgtctatgttccagttacaactgtaatggcagtcgcc
ccaataggagcttctgctaaacgaagaagggttgacgatggacggccatcgacttcttcgacaatcgtaagagtcgtttcgcgtcttgctactgttggaa
cgcagaccgacgtcaaagtgagtatggtcgatacttccaccgtgacatgcaattgctctacacaaacaaaaagaattattactaaggaagtgagttctcc
aagtggagaagatttcatccagtcagatgttcatcaaaatcgccatatcctgtgtgttttttaattccttccaaaccaaccagcaaaattcaaaataaaa
tcaaagtgatctgcaatcaacgatgcattcaacaccagcaccaatggagtccttaatagttgaggtagaaaaaaatttggatggcagtaggttgagtgtt
cgggcaacatgtttagatcataattcactgctatggaactcacagcccacctttggtaaaacttcaacaggaaagtttttactgaaca
3' EST: CX839923 [+]caatgttttattaacaagcatgaaaaacatttttttaactgcacaaatagatcagtctgacacgtctcatgaaaaagggcttttcccaaaactgatttaa
tgtaaagagaccctttatagcattacccagtgtccatcaggagtctttagctgcaaagagttggtctaagggttctcttaaagaaacatttcatggtgga
cataaaaatatttgcctgatgcctcatgtgtaaagtacagtttttctcatgcgaaagagtatttttagattactgttcaatgcaaataatttccctcatt
cagaaaatggtttctcccctgtgtgagtcttttgatgccgatcaaggcaagagtgctttgaaaaacatttcccacattaagaacaagaaaatggtttctc
ctctgtgtgggttctatgatgacgatcaaggttagatcgacttgtaaaagatttcccacattcagaacaatagaatggtttctctcctgtgtgagttctc
tgatgacgatcacggtgatattggttagaaaaacatttcccacattcagaacaatagaatggtttgtcccctgtgtgagttctctgatgacgatcacggt
gatatgggactgaaaaacatttcccacattcagaacaagagaatggtttctcccctgtgtgagtcctttgatgacgcgcaagatagaaatggtttaaaaa
acatttcccacattcagaacaagaaaatggtttctcctctgtgtgagtcctttcatgacgagcaaggttggattgatttgaaaaacgtttcccacattta
caagaaaaaaggtttgtctcctgtgtgggctctatgatgaatg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: