XB-CLONE-3300622
Clone Name: IMAGE:7660124
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Gene:
tcf3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136070 [+]
GTAGCATTGGAACTAGTTACAGTTGCTGTTTCTCAGACAAGAAGACAGACCGCTGCACCCCCCTCCTCCTCCCAAATAACCCCCCCAGTCAGGAGGGGGG
CACAGGAAGGACCCCAGAAATCATCAAACAGCCCCCTGCTCTGCTTTCAGCTTGAGGATAGTATAGCTGCGCCCAGACCTGCTTCTCCTGGGGGACTTAT
CCACTTCCTATCTCCATGCAATCCTTCTGGATGTTGACTTTGTGAAAAGGGGTCTGCCTTTTAATTGTGGAGGAGAATGAATCAACAGCAGAGGATGACA
GCTGTGGGCACAGACAAGGAGTTAAGTGACCTCCTTGACTTCAGCATGATGTTCCCTCTACCTGTAGCTAATGGAAAGACCAGGCCTACTACCCTTGCTA
GCACACAGTTTGGAGGATCAGGTTTGGAAGAGAGAACAGGCTCTGGACCATGGGGTACAGAAGATCACAATGGATCTAACTTTGACCAAGGAAGGGGTTA
TGGAGATGGATCACATTATGGGGAGCACAGGGAACTGCCATCACATGAAGGGAACTTATCTGCTCCTTTCCTTGCATCTGGTCTTGTAGGAAAAAATGAA
AGACCTCCATATTCTACTTTTGGAAGAGACTCTGGGATGGCAATGAATCAGACTGGATTTCTTCCTGGAGACTTGGCAATGAACAGTCCTTCTGCTCTGT
CACCAAATGCAGGAAAAGCAGGCTCACAATATTATACTTACCCCAACAATTCAAGGCGGCGACCAGGAGACAGTAACCTTGATGCCCAGCCAAAAAAAGT
GCGAAAGGTACCACCAGGACTACCTTCTTCTGTTTACCCAGCAAACTCAGGTGATGAATACTGTGGTGACCGAGGAAGTTACGCCAACCCCAAGGCACCT
AATTCAGTGTATCCTGGTGCATTCTACATGACAGATGGACTTCACAGTTCCTCTGATATCTGGGCTTCTTCAGGAGGGATTGGACAAGGAGGATACAGTT
CAGTCCTGAATAGCTCTCAGTCCTCCGTTTCCCAGACCACAGGATTCAGCAGCCTGCATCCCCATGAACGAATGGGTTTTCCAATGCATTCTGGGGAGGT
GAACCCTTCTGTGACTTCCAGTTTCTCCACTACCCCTGCACAGTATGGGGTTTCCAGCCATACACCCCCAATCAGTAGTGGGGACACTATGATAGGAAAC
AGAGGGACAGCCACTGGGAGCTCAGGAGATGCACTTGGAAAAGCACTAGCCTCAATCTATTCTCCAGATCATTCCAGTACCAACTTTTCCTCCACCCCAT
CCACACCTGTGGGTTCACCACAAGGAATAACAGGTTCAGGGCAGTGGCCAAGAGCAAGCGGTCCTGGAGCTTTGTCTCCTAGTTATGATGGAACACTTCA
CACGTTACAGAACAAGATGGAAGATCGCTTGGACGAAGCCATCCATGTTTTGAGAAGTCATGCTGTGGGGCAGACAGGCTCCCTTCCAGGAGGCCATGAG
GACATTCACTCTCTTCTCAGTGCTACTGCTAGTGTTCTTGGAAGCGGCTTCCCTGCTGCTGTACTCTCTCTTGCCAATCGACATTCTATGTTAAACTCTC
ACACAGAAGAAGCCCTGCCTAGTGCTTCCAACATGCTACACAGTCAGGTCACTGTTCCTGCACAGCCAGGTTCACTGCCTGATCTAACCAGGCCACAGGA
TTCCTACTCAGGGCTTCCTGGGCTTGGAAGGGCAGTACCTCCAGGCAGAGCCCCAGAGATAAAAAGGGAGAGTAAAGAGGATGAGGAGAATAGATCTGTG
GGAGATCTGTCAGATGATGAGAAGAAAGAGTCTAAGACACAGAGAAGTCGGACACGATGCTCAATAAACAGTGGAGGAAGCAGTAAAGAAGAGAGTAACT
CTGTGGAAGAAAAGGACTCAAGGGATCGTGACAGGAGGATGTCAAATAATGCACGGGAGAGGGTGCGAGTACGAGACATCAATGAAGCTTTCAAGGAACT
TGGTCGTATGGTGCAAATGCATATGAAGGCAGACAAGGCCCAAACCAAGCTCATCATCCTTCAGCAGGCCGTGCAGGTCATAATGACTCTGGAGCAGCAA
GTGCGAGAGCGCAACTTGAATCCTAAAGCTGCCTGTCTCAAGAGGCGTGAGGAAGAGAAAGTTTCAGGTGTTGACCCTCAAATGGGCCTGTCTGGAGGAC
ACCCTGGAGTTGGGGACTCTCACAACCCAGTGGGACACATGTGAGTTTCCACTGGAGCACTGGAACATTTTTCTCTACGATCCTTTGTTGCTGTTATTGT
TTTTTTTATATTATTACAAATTTACTTTTGAATTTTTTTTTTCTTTGTGTTATGGAAAACCTCCACGACACGGAAGTCTTTGTGGCCTTGACTTTATTTT
CCTGGAAATTTCCAATGGGGGATGGACAAAAAAAAACATTGACTCAAACTGACAACTTGTCTTTTCTGGACTCTTACAAAGCAAAGGAAGACAATGTGAG
AAATTGCCTTAACTGAAAACAAAATCAAATCGGTTTATTTAAAGGAGGCTGCGGGGGGGAAGAAGGCTTGCCCAGATACTCCCTGCTACTAGTGAGTTGT
GGGGAAGCAAGGATATTTTTCTGTCCTTGTACTTACACATCTACAACAAACTGACTTTTTTTTAATGAAATGAAAAATGGAAAACTGCGAAAAAAAAAAA
AAAA
5' EST: CX840496 [+]tagcattggaactagttacagttgctgtttctcagacaagaagacagaccgctgcacccccctcctcctcccaaataacccccccagtcaggaggggggc
acaggaaggaccccagaaatcatcaaacagccccctgctctgctttcagcttgaggatagtatagctgcgcccagacctgcttctcctgggggacttatc
cacttcctatctccatgcaatccttctggatgttgactttgtgaaaaggggtctgccttttaattgtggaggagaatgaatcaacagcagaggatgacag
ctgtgggcacagacaaggagttaagtgacctccttgacttcagcatgatgttccctctacctgtagctaatggaaagaccaggcctactacccttgctag
cacacagtttggaggatcaggtttggaagagagaacaggctctggaccatggggtacagaagatcacaatggatctaactttgaccaaggaaggggttat
ggagatggatcacattatggggagcacagggaactgccatcacatgaagggaacttatctgctcctttccttgcatctggtcttgtaggaaaaaatgaaa
gacctccatattctacttttggaagagactctgggatggcaatgaatcagactggatttcttcctggagacttggcaatgaacagtccttctgctctgtc
accaaatgcagganaagcaggctcacaatattatacttaccccaacaattcaaggcggcgaccaggagacagtaaccttgatgcccagccaaaaaaagtg
cgaaaggtaccaccaggactaccttcttctgttttaccagcaaactcaggtgatgaatactgtggtgaccga
3' EST: CX840495 [+]tttttttttttttttcgcagttttccatttttcatttcattaaaaaaaagtcagtttgttgtagatgtgtaagtacaaggacagaaaaatatccttgctt
ccccacaactcactagtagcagggagtatctgggcaagccttcttcccccccgcagcctcctttaaataaaccgatttgattttgttttcagttaaggca
atttctcacattgtcttcctttgctttgtaagagtccagaaaagacaagttgtcagtttgagtcaatgtttttttttgtccatcccccattggaaatttc
caggaaaataaagtcaaggccacaaagacttccgtgtcgtggaggttttccataacacaaagaaaaaaaaaattcaaaagtaaatttgtaataatataaa
aaaaacaataacagcaacaaaggatcgtagagaaaaatgttccagtgctccagtggaaactcacatgtgtcccactgggttgtgagagtccccaactcca
gggtgtcctccagacaggcccatttgagggtcaacacctgaaactttctcttcctcacgcctcttgagacaggcagctttaggattcaagttgcgctctc
gcacttgctgctccagagtcattatgacctgcacggcctgctgaaggatgatgagcttggtttgggccttgtctgccttcatatgcatttgcaccatacg
accaagttccttgaaagcttcattgatgtctcgtactcgcaccctctcccgtgcattatttgacatcctcctgtcacgatcccttgagtccttttcttcc
acagagttactctcttctttactgcttcctccactgtttattgagcatc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: