XB-CLONE-3300387
Clone Name: IMAGE:7660417
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Gene:
mysm1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC153337 [+]
CGAAGAAGCGCGATATATGTGCAAAACGTATATAAATTAACCTATTGTATGCTCTTCTATAGGCAGCTCAAGTGAAAGGTTTACCATTTTAAAGGTTTAC
TTCCCCTTCACAGGCCTGGAAAACGAGGAGGGAACGTAGCTCCTAAGGGTCGTCCCTTAACTCCGCCCCTCCAATCATGGCCGAGGAAGCAGCGGATGTT
GATATTGACATAGAGGGAGATATAAATGAATCCGGCAAAAGTATTTATGACAGCCTCAGTACTGGATTTGCTCCTGAGCACTACATTGAATCAGAGTGGA
AGAATGAAGGCCTTAATCCATGGACATTGGACAGTTCAATCAGTGAAGAAAACAGAGAAGCAATTAAGAAAATGCTGCTTGAGGAACAATATTATCTATC
TAACAAGCCACTAGCAGGGAAGTTTTGGTCAGATTCTAAAGAAGGAGAAAAATGCTGCATCAAAAGGGTACGAACCCCTGCTAAAACCAGCTGTAGCCCA
GTGAAATGGACTACAGAAGAAAAAAAACTCTTTGAGCAGGGGCTGGCTACTTTTGGCAGAAGATGGACAAGTATTGCAAAGCTGATTGGGAGTCGAACTG
TATTACAAGTAAAAAGCTATGCAAGGCACTACTTTAAAAATAAGTGTAAGCTGGAGGGTTTTGTCAAAGAGGAGGCAAAGATTGGCACTGAAAGTCTTCA
GATACTTGATTTTCAGGACCATGAAAATGAACCTGAAATAACAGATGAAAAGCCAACCTTACGTGGACGAGCCGACCCAAACCTAAATGCTATCAAGATT
GAAAAGCTGTCTGATGATGAAGAGATAGACATAACGGATGAGTTGGATGAACCACTTCGTAATAATCCAGATCTTGTTAAAGACATAACTGATACAACAG
ATGCAAATAAAGAGAACAAGCTAGAGACAGACATTGTACAACAAAAAAGTAATATCACAATAGAAATTTTGCAGACTGATATTCCCAAACATGTGCTGCA
ACAAAGCGACATTGAATGCTTGGATTCTGTACATTCATTAACTTGTATACCCTCCCCAAAATGGATGTTGTATCCACAGCAATGCGAAGATGATGATTAT
GATCATTCAGATGAACAGCATTGTTTCCAAGAGAAATGCCTCTCTCCTCATGCAGAACATCTAACTGATCATCCAGATGAACAGCATTGTTTCCAAGAGA
AATGCCTCTCTTCTCATGCAGAACATCTAACTGTATTATGCGAGAATGAAAATGATTCTCAAGACGAAGATGATGAGATAAAGCCACCAGACCAAGATAT
AGAAATTGACAGAAATTTAATTTTAGATGAAGAAAAACAAGCAATCCCAGAGTTTTTTGAAGTAGACAAGCAAAAACTCCAGATCGTTACCTGAGGATCC
GTAATTATATTTTGGATCAATGGGAAAATTGTAAACCCAAATATTTGAATAAGACCTCCGTTCGTCCTGGTTTAAAAAACTGTGGTGATGTGAACTGTAT
TGGACGCATACACACTTACTTGGAATTAATTGGTGCAATTAACTTTGGCTGTGAGCAAGCTATCTACAACAGACCTCGCCCAGTAGATAAAACAAAGGGC
AAAGAAGGAAAAGACACTTTGGAGGCATACAAATTAGCACACCGACTGCAATCTATGCGCACTAGAAAGAGGCGTGTCAGAGACCCCTGGGGAAACTGGT
GTGATGCAAAAGATCTTGAGGGACAGACATATGAGCATCTCTCAGCCGAAGAATTAGCTAGGAGACATGAGGAGAAAATCAAATCTTATAAATATTCCAA
AGGAACAAGACAAGTCAAGAGCTCATTTGATCCTTTCCAGTTGATCCCCTGCTCTACATTCAGTGAAGAGAAAAAGGCGCCATTTCAAGTGAAAGTAACA
TGTGAAGCTATGCTAATATTAGACCTGCATGCACATGTCTCCATGGCTGAGGTGATTGGCTTATTAGGTGGAAGGTATACAGAATCTGAAAGTGTTGTAG
AAATCTGTGCAGCAGAGCCATGCAATAGTCTCAGCACCGGACTGCAGTGTGAGATGGACCCTGTCTCACAGACACAAGCATCAGAGGCACTGGCGTCCAG
AGGATACAGTGTAATCGGATGGTACCATTCACATCCTGCTTTCGATCCAAATCCCTCTATTCGAGATATTGACACACAGGCAAAATACCAGAATTATTTT
TCTCGTGGTGGAGCAAAATTCTTGGGAATGATAATAAGTCCTTACAATCAAAGAAATCCTCATCCCCAGTCCCAGGTGTCTTGCCTGATTATAAGTGATG
AGCTGAGCAAAGATGGGTCTTACAGAATACCTTACAAGTTTGATGTTGAATACATGCAAGGAGAACTACAGTGGGAGCTGGTTTTTGAAAAGACAAGATG
GATAATAGAGAAGTACAAATCATCTCACAGTAGTGTTTCAATGAATAAAAGATTCCGTCATGACAGTGAGCTGACATGTTTACAGAAGCTTTTGATATGT
ATGAAGAAAACTTTAGGGAATACAACCTGTCCTCTTTTAACAGAAGAATTACTACTACATCGTATAGAGGAATTCTTTCTAACCAGTTACAACACCAGCA
GAAAAAGCAATTATGAGACTGAAGAACATGATCCGTATAAACAAAATGTTGATACCATAGTAGCAAGGGACAGTTTCCAAGACTATTCAAAGGAACCAAA
TGGACACCAATCTCTTTAGAGTCTCTTCCAATTCAAGATTGCTAATGTATTGGTAAACACAGTACTTTGGATTTACACACACACAAAAATTGCTGAATAA
TAGAAAAAAAGGTCCAAGATGTTCTTCTAGGGATGCTGTCATGTTTATTTCTACTTTATCCTTGAATATACAAATTTGTTTAACAAATGTGGAAATAGTA
GCAAAAGCTATTTCCCAGAGTTTCCCTAATTTTGACTTATAGGGGTTTCAGCCACTCAGTATCAATAGTAAATCAGCCCCACTTCTGTGATTCCACGGCC
AGTCTGACAGACTTTGCACTTCATTTTAAATTTGTGCTATTAAAAGGTTTTAGCATGTTCTTTTTTGTCAAGGCATTGTGTTACGTGGCAGTGTTTGGGG
TTTTTATAGTGTAATGAGTAAGTTTTTGCTATATGGATGACAAGTAGGCCTGGATAAGCTGTGGGAGATGTTTGTGTTTAATTAAATTAGCATTTATACA
CATAATTCTCTGCTATTTTCAAGGGATAACACTGCCTTTTTGCACAAAAGAATGGTGCAACAACCCCATTTCCTCTGCATATACAGCAAGCCCAGCCTGG
AAGTTCATATGGCTTTACCACTGAGCTGTGTTTTGAAAGAAACCGGAGACATTAACAGGAAAAAAAGAGGGAGAAAAAAAAAAAAACAATTGTGAAAAAA
AAAAAAAAA
5' EST: CX840896 [+]cgaagaagcgcgatatatgtgcaaaacgtatataaattaacctattgtatgctcttctataggcagctcaagtgaaaggtttaccattttaaaggtttac
ttccccttcacaggcctggaaaacgaggagggaacgtagctcctaagggtcgtcccttaactccgcccctccaatcatggccgaggaagcagcggatgtt
gatattgacatagagggagatataaatgaatccggcaaaagtatttatgacagcctcagtactggatttgctcctgagcactacattgaatcagagtgga
agaatgaaggccttaatccatggacattggacagttcaatcagtgaagaaaacagagaagcaattaagaaaatgctgcttgaggaacaatattatctatc
taacaagccactagcagggaagttttggtcagattctaaagaaggagaaaaatgctgcatcaaaagggtacgaacccctgctaaaaccagctgtagccca
gtgaaatggactacagaagaaaaaaaactctttgagcaggggctggctacttttggcagaagatggacaagtattgcaaagctgattgggagtcgaactg
tattacaagtaaaaagctatgcaaggcactactttaaaaataagtgtaagctggagggttttgtcaaagaggaggcaaagattggcactgaaagtcttca
gatacttgattttcaggaccatgaaaatgaacctgaaataacagatgaaaagccaaccttacgtggacgagccgacccaaacctaaatgctatcaagatt
gaaaagctgtctgatgatgaagagatagacataacggatgagttg
3' EST: CX840895 [+]tttttttttttttttcacaattgtttttttttttttctccctctttttttcctgttaatgtctccgggtttctttcaaaacacagctcagtggtaaagcc
atatgaacttccaggctgggcttgctgtatatgcagaggaaatggggttgttgcaccattcttttgtgcaaaaaggcagtgttatcccttgaaaatagca
gagaattatgtgtataaatgctaatttaattaaacacaaacatctcccacagcttatccaggcctacttgtcatccatatagcaaaaacttactcattac
actataaaaaccccaaacactgccacgtaacacaatgccttgacaaaaaagaacatgctaaaaccttttaatagcacaaatttaaaatgaagtgcaaagt
ctgtcagactggccgtggaatcacagaagtggggctgatttactattgatactgagtggctgaaacccctataagtcaaaattagggaaactctgggaaa
tagcttttgctactatttccacatttgttaaacaaatttgtatattcaaggataaagtagaaataaacatgacagcatccctagaagaacatcttggacc
tttttttctattattcagcaatttttgtgtgtgtgtaaatccaaagtactgtgtttaccaatacattagcaatcttgaattggaagagactctaaagaga
ttggtgtccatttggttcctttgaatagtcttggaaactgtcccttgctactatggtatcaacattttgtttatacggatcatgttcttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: