XB-CLONE-3299965
Clone Name: IMAGE:7660981
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Gene:
XB5970036
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167589 [+]
gtctcagttccttcccttgaatgttttgaccggatggagctgagggaccttcctgaggagccggttggaaattgtgttacactagactgcacctacagtg
acactggaatatgaaggaccatgtgaactctgtaaagagtgagatggacccagtgtctgaggctgaaaatgaaccagaaattttacagataaaaatcaaa
gaagaagaacctgactttgaatatgatgagaaccagatggaaaattctacggtttcacttactgatgggggttctgagaatgaacctaaagtgaaatctg
aggacgaagagtcggacactgaagatactgtgactgcaataaagagggaaacggattccgttcctggggccgacgactgcataaatcactcaagggacag
tgaccctgaaacagccagaacagaagtttcagaagccaatgaccacttgagtacattggatgcaaccaaggagcctttacagtgtgctggagtattggca
ggaccggcacagcacaaagcactaacctccagcgctaaatataaggattgctcagccggctctgaagcatcagcttccatctgctctaaatgtggagaga
gtttctcggagaatagcaatctgcttgctcatgtctgcactgaatctggaaatgataataatcatactgagagatgtgtaggtgaaaatacaaataataa
acctttttcctgtgtgatatgtgagaagcattacagctgtaagtctaaactacagaaacaccagttggtccatagcggagcgaaacgttttgcatgtacg
gaatgtgggaaaagcttctataccaacagccacctccagagacacctgatcattcacacgggggagaaaccattcaaatgcacggaatgtgggaaaagct
tcaatgtgcagagcaacctgtacagccaccagaaactccacaaaccagaaaagccttataaatgtgctgattgtgggaaaagcttttctctaaagggcaa
tcttaacatgcacctgaggattcacactggggaattaacttgtaccgaatgtgggaaatgtttcattaaaaagacggactttcagaatcacctgaaaagt
cacacaggggagaaacccttcacttgcactgagtgtgggaaaagttttatcaaaaaaagctgctttcaaaaccaccagaaaacccacacgggagagaaac
ctttcaggtgtaccttttgctggaaaagcttctgcagcaaacagacccttgtgacacacctgagaatacacacgggggagaagcctttctcctgcataga
atgtgggaagtgttttcctaggaagagctccctccgtaaccaccagaaagggcacacaggggagaagcctttctcgtgtacagaatgtgagaaaagcttc
tatgataagcgcagcctttccaggcaccacagcgttcacacaggggccaaacccttcacatgtaaagactgcgggaaaagcttctcacagaagtgcagcc
tgcacactcaccgctacatttgcaaagggtctcgaaacactggaggtaaagtgttatcagagaataagcagcaggatcggaagaaacgtctgactgtaat
aaagaggcaaaaggattccagtccagaggcagagaccgaagctaaagtgaaatcagagactgaagagtcggacaccgaggaccatgtgactgtaataaag
atggaattggattctgttcctggggccgaaaactggctaaatgatgaaaagaattgggagtctgaaccccccacaccttggttatgtcagttgccagaag
ccagtgactgctcagatacattggatgcagccaaggagccattacaggaggctggagtattgctggggccggctccgcacaaagccctgccttattctga
ggctggcacaagatatgaggattgctcagcaggccccaaggcatctggctttatctgctgtaaatgtgggcaaagtttttctgttaagagggaccttctc
actcatgactgtaaacagagtggggacaataacaacaaaccaatgatagacgatgtaaaagaagagcctagtagccataaatctcatagacaccttcggc
caataaaacataaattgggccacacagggggggaaccgttcacatgtacagagtgtggcaaagccttttctcaaaagagtcaccttcacagacaccatat
tgtccacacaggggagaaaccattcaagtgtgcacagtgtgggaaaagattctctcacaatggaaaccttcttagccacctgaaaatacactcaggggag
aaaccattcacttgtacagaatgtggcaaaaccttctctcgcaagagcagccttcgcagccaccagaaagtccacacgggggagaaatctttcacttgta
tagaatgtggcaaaagcttcggtaacagaagctaccttcaccttcatcagaaaatccatgcaggaaagaaagctttcccctgtaccgaatgtggcaagag
cttctatgtcaagcgaaacctttggaggcaccaaaccattcacacgggggagagagatttcacatgtagagagtgcggcaaatgtttttctcagaagaac
aatctttacaaacaccaggccgttcacacaggggttaaacccttcacgtgcacagagtgtggtaaaagtttctctcaaaagagtcacctgcatagtcacc
tgaacattcacacaggggtgaaaccctacacctgcgcagagtgtgggaaaagcttctcccacaacagaaacctttacagtcataaaaaaatccacacggg
ggagaaagcatttacatgcacagactgcggaaagtgcttctcacagaagagtaatcttcttaaacaccagacggtgcacacaggggagaaaccatttaca
tgcacagattgtgggaggagcttctctcagaagagccagcttcaccgacaccagaatatccacacaggggcaaagcccttcacctgtacagaatgtggca
aaagcttctctctaaagtgcagtctccagagccaccagaggatccacacgggggagaaaccatttgcatgctctgaatgtgggaaaagcttctatcagaa
aagcaatctaagcaaacaccaggccattcactcagaggagaaaccattcacctgctcagaatgtgggaaaggctttgctgttaagcgcaacctgcagcga
catcagaatactcacgcagccagaagccgtagaagcactgagaaggttcattagtgaagtcagacaatgaagagtcggacgctgaggatcatgttactgc
agcgaataaggaaagggtttctcttcccagagccgataatggcagcagtcggaattctgagacccccagatcttggcgaagacgccggaatgttattgtt
acagaagccggtgatacattggatacagccaaggagccattgcaggagtccggcactagatatgacaattgcattacaagctctgattcatcaggtttta
tttgcaataaatgtgggaaaagcttctctctgaattgcgatctcctgacccacctctgttcccaagatgagaaagatgagatatctaatatacgttccag
agagaatgcagaagagaagccttacttctgtgcagtgtgtgggaagcactacagtcgcaagtcacattgccagagacatcagctggtccacacaggggag
aaaccattcacatgctcagtatgtgggaaatgcttctctcttaagagcaatctgcaaagtcacgagcgagttcacacaggggagaaaccatacacgtgtt
cagaatgcggcaaaagcttctctcataaaagcatcctaaaccgacatcaaaagactcacacgggggagaaaccttgtaaatgttcagaatgtgggaaaag
cttctctcacagcagccaccttcacagacaccagaagatccacacgggggagaaaccattcacctgcacggaatgtgggaaacatttctctcgaaaaggc
aatctgcagagccaccagaccactcacacgggggagaaatcttttgtttgcacggaatgcggcaaaagcttctctcaaaaaagcaacttgtacagtcacc
agaaaatccacaccggggagaaaccctacacgtgcacagactgtggcaaatgcttctcacagaagggcactctgtacaaacaccaggccattcacacggg
agagaagcggttcaaatgtattgaatgtggcaaatgcttcacacagaagtgcactctgtacaaacaccagactgttcataccgagaggaacatattacat
gcgcagaacttaggggaagatatatgagcagtgctcttacgccaatggaacaacagacgaatagaaacaaaggggaatatttgtcgggctctgtaaaaag
tggagtaggacattgagggtgatgttgcccagggcaaccaatcagcatttagatttcaacagttagcaaccaatcagatgctttgctttggttttctatg
agcagcatcgccggtgatgttttactccactttttacacagcctgataaatatacatgtaccagtaactatgtagcacggctgtaggccacggggcttcc
gagctgcactaaatcatgttcttagattcgtagaatgtaggataatgagtttaattggaattattacactagatcagggttaacaaagaccaatgagggg
agcagtaagcccagttagtatagacacatctcggtgacatatgaaataaaacctatactttgtaaataacctgtgtatagtgtatggcctgactcagtac
agcagaatgcatcaggctttccagtacttccagtacagatccatgagaacaacagggaaaactctgcatacaataaaaatgccaatatatccattgataa
aaaaaaaaaaaaa
5' EST: CX841521 [+]tctcagttccttcccttgaatgttttgaccggatggagctgagggaccttcctgaggagccggttggaaattgtgttacactagactgcacctacagtga
cactggaatatgaaggaccatgtgaactctgtaaagagtgagatggacccagtgtctgaggctgaaaatgaaccagaaattttacagataaaaatcaaag
aagaagaacctgactttgaatatgatgagaaccagatggaaaattctacggtttcacttactgatgggggttctgagaatgaacctaaagtgaaatctga
ggacgaagagtcggacactgaagatactgtgactgcaataaagagggaaacggattccgttcctggggccgacgactgcataaatcactcaagggacagt
gaccctgaaacagccagaacagaagtttcagaagccaatgaccacttgagtacattggatgcaaccaaggagcctttacagtgtgctggagtattggcag
gaccggcacagcacaaagcactaacctccagcgctaaatataaggattgctcagccggctctgaagcatcagcttccatctgctctaaatgtggagagag
tttctcggagaatagcaatctgcttgctcatgtctgcactgaatctggaaatgataataatcatactgagagatgtgtaggtgaaaatacaaataataaa
cctttttcctgtgtgatatgtgagaagcattacagctgtaagtctaaactacagaaacaccagttggtccatagcggagcgaaacgttttgcatgtacgg
aatgtgggaaaagcttctatac
3' EST: CX841520 [+]tttttttttttttttatcaatggatatattggcatttttattgtatgcagagttttccctgttgttctcatggatctgtactggaagtactggaaagcct
gatgcattctgctgtactgagtcaggccatacactatacacaggttatttacaaagtataggttttatttcatatgtcaccgagatgtgtctatactaac
tgggcttactgctcccctcattggtctttgttaaccctgatctagtgtaataattccaattaaactcattatcctacattctacgaatctaagaacatga
tttagtgcagctcggaagccccgtggcctacagccgtgctacatagttactggtacatgtatatttatcaggctgtgtaaaaagtggagtaaaacatcac
cggcgatgctgctcatagaaaaccaaagcaaagcatctgattggttgctaactgttgaaatctaaatgctgattggttgccctgggcaacatcaccctca
atgtcctactccactttttacagagcccgacaaatattcccctttgtttctattcgtctgttgttccattggcgtaagagcactgctcatatatcttccc
ctaagttctgcgcatgtaatatgttcctctcggtatgaacagtctggtgtttgtacagagtgcacttctgtgtgaagcatttgccacattcaatacattt
gaaccgcttctctcccgtgtgaatggcctggtgtttgtacagagtgcccttctgtgagaagcatttgccacagtctgtgcacgtgtagggtt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: