XB-CLONE-3299748
Clone Name: IMAGE:7660784
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Gene:
pou6f1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC153338 [+]
AGAAGGGGGACAGAGCTACAAATAACATGATGAAAATGAACTGTGCTACATGTGACCTGCAATGTTTTATTAACTAAGAAATGTTGCTTCAATAGCCAGA
TACTATAAAAAGAAACTAGAATGGAAATCAACCACATACGAAGGTGTTTTCTTCTTCTCAGGATGCAGGAAGCATAAGGGCTGTGTAGTCCAGACTTGAG
GATATGGATGCCGGAACCTTACAGCAGCATGATGGATCACTTACTGTCAATGAACAGGTGATTGTTATGTCTGGTCATGAGACCATCAGGGTGCTTGAAG
TTGGTGTTGATGCTCCAGTGCAGTCTGAGGAAAGTGGGAAAGCTGCATCTGAACCTGCTGACACAAGTGATATGACAAGGGACAATGTGCTATGTCATGC
AGAAAACACAGGTACTGAACCAACAGTAAAATCAACAGCTGGATTAGTAACTACTTCTATCCCAACTCTTTTCACCCACGCCTCAAGCCAGCCACAGCCC
ACTATTGCATCGTTTGCGGTGCAACCTGCACCCCAGGTTTTGACTCAGGAAAACTTAGCTACACTTGTGACAGGAGTTATGGCTCCAGCAGGGCCAGTTG
CTCAGCCTGTTCTTATCCCAATAAGTATTGGTGGTCAAGTGGCCGGTCAGCAGGGTCTGGCTCTCTGGAACTTTCCCACTGCAACAGTTGCTACATTGCC
AGGTTTAACAACCGCTCCTCCTGCTGGTGGTGTTTTTAAACTGCCTTTAGCCAATCTGCAAGCTTCACTTTTGAATACTGCTTTGCAAGCTCCAATGCAG
GCTGCCCAGGCCATTCAGACTGTTTATCAAACACAACCAGCACTCCAGGCCCAGCCTTCCCTACAGACAGCTGTTCAGCCAATGACCACTCTGCAAGCTG
CTTCACCTTTACAGGGGCAGACAGCTGTGCTGCCAGGCACACTACAGAAAGCAGCCGAGCCACAGTCGCAAATCACAGTACAGCCAGCAAGCTTCACCTT
CAATCCAGCCATTATCAGTGCAGCTTCTCTTGGTGGTCAGGCACAACTGCTAGGCTCGCTGGCCGCCACTCCAATAATAGCCAATGCCATTCCTGGAGTA
CAAGGGATTACAAGCCAGATAATAACCAATACCCAGGGCCAGGTTATTGGTACCCTACCGTGGTTGCTGAACTCACCGGGGATCACTGCAGGTGCTGCAA
CAGCTGCTGTCTCTGCTCAGAATCTGGTACAGACCGTGACTCCTCAGTTGCTGCTAAATGCCCAAGGGCAAGTCATTGCTACTCTGGCTAGCAGTCCCAT
ACAGCAAGGTGCTGTCAGAAAACAAACTCCTCAAGATTCACCAGCTCACCCCAAGTCAGAACAACAGGTAATCCAACCTACACCAGCTATTACGCAACCT
TCTGTGGTTGTGGCAAATCCAGCTCCGCCCACCACATCAACTTCTACGCAAGTCCATATCAGTTGTTCTGAGCCCCCAACTGTGGGTCAGCTAGTTTCCA
AAGCACATAGTGGGAACTATGATGACGATGGAATAAACCTGGAGGAAATACGTGAATTTGCTAAAGAGTTCAAAATTCGACGCCTGTCTCTGGGTCTGAC
GCAGACACAGGTGGGCCAAGCCCTGACTGCCACTGAAGGACCAGCATACAGCCAGTCTGCCATATGTAGGTTTGAGAAGTTGGACATTACACCCAAAAGT
GCTCAGAAGCTGAAGCCTGTATTGGAAAAATGGTTAAATGAGGCAGAAATGAGGAATCAAGAGGGACAGCAGAATCTAATGGAATTTGTAGGTGGAGAAC
CTTCAAAGAAGCGAAAAAGACGTACCTCTTTCACGCCACAGGCAGTAGAGGTTCTGAATGCCTACTTTGAGAAGAATTCATTGCCTGCTGGGCAGGAAAT
CACAGATATTGCTAAAGAGCTTAACTATGACCGAGAAGTTGTTAGGGTGTGGTTCTGTAACCGGCGCCAAACTCTGAAGAATACCAGCAAGCTCAATGTC
TTTCAGATCCCTTAGAAGGGAATAGGCAGTCTTGCTGTCACCACCTTATCTTCTTACATTTAATGGTGGTGATGCTATGTGACATTCTTTTGTGAAAGCA
CTTTGTGACTTGATTTTGGCTTTCTGTGTTATAACGAGTTGCCCATTGGAGCCAACCCCTTTTCCTTTGTATTATTTTGCAACAGGACAGATGCCAAGCA
TGACAAGAGGTCGATAGCCATCACAGCGGGATGTCTCCGGTCAAATTGTGTTCCCTCTGTGGTGGGCAAATATCAGCTTCTGCAAAAAAAAAAAAAAA
5' EST: CX841829 [+]agaagggggacagagctacaaataacatgatgaaaatgaactgtgctacatgtgacctgcaatgttttattaactaagaaatgttgcttcaatagccaga
tactataaaaagaaactagaatggaaatcaaccacatacgaaggtgttttcttcttctcaggatgcaggaagcataagggctgtgtagtccagacttgag
gatatggatgccggaaccttacagcagcatgatggatcacttactgtcaatgaacaggtgattgttatgtctggtcatgagaccatcagggtgcttgaag
ttggtgttgatgctccagtgcagtctgaggaaagtgggaaagctgcatctgaacctgctgacacaagtgatatgacaagggacaatgtgctatgtcatgc
agaaaacacaggtactgaaccaacagtaaaatcaacagctggattagtaactacttctatcccaactcttttcacccacgcctcaagccagccacagccc
actattgcatcgtttgcggtgcaacctgcaccccaggttttgactcaggaaaacttagctacacttgtgacaggagttatggctccagcagggccagttg
ctcagcctgttcttatcccaataagtattggtggtcaagtggccggtcagcagggtctggctctctggaactttcccactgcaacagttgctacattgcc
aggtttaacaaccgctcctcctgctggtggtgtttttaaactgcctttagccaatctgcaagcttcacttttgaatactgctttgcaagctccaatgcag
gctgcccaggccattcagactgtttatcaaacacaaccagcactccaggcccagccttccctacagacagctgt
3' EST: CX841828 [+]tttttttttttttttgcagaagctgatatttgcccaccacagagggaacacaatttgaccggagacatcccgctgtgatggctatcgacctcttgtcatg
cttggcatctgtcctgttgcaaaataatacaaaggaaaaggggttggctccaatgggcaactcgttataacacagaaagccaaaatcaagtcacaaagtg
ctttcacaaaagaatgtcacatagcatcaccaccattaaatgtaagaagataaggtggtgacagcaagactgcctattcccttctaagggatctgaaaga
cattgagcttgctggtattcttcagagtttggcgccggttacagaaccacaccctaacaacttctcggtcatagttaagctctttagcaatatctgtgat
ttcctgcccagcaggcaatgaattcttctcaaagtaggcattcagaacctctactgcctgtggcgtgaaagaggtacgtctttttcgcttctttgaaggt
tctccacctacaaattccattagattctgctgtccctcttgattcctcatttctgcctcatttaaccatttttccaatacaggcttcagcttctgagcac
ttttgggtgtaatgtccaacttctcaaacctacatatggcagactggctgtatgctggtccttcagtggcagtcagggcttggcccacctgtgtctgcgt
cagacccagagacaggcgtcgaattttgaactctttagcaaattcacgtatttcctccaggtttattccatcgtcatcatagttcccactatgtgctttg
gaaactagctgacccacagttgggggctcagaacactgatatggacttgcgt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: