XB-CLONE-3299573
Clone Name: IMAGE:7660772
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Gene:
mpp2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136159 [+]
GAGCTATCAGGACCTAGGCACCCCAGGCCCTTTATATCCTGCCATCCAGACTGAGAGGGGTACCTGCGGATCTTTAGGACCACTCAACATTTGTAATATT
TTCTTGCTGGTACATCCTCTATACCATCTATTCTCTTATTATCCAGGAATCTCTTGGGTACCCAATGCCTGTCGCTCTGCCTTCCCACAACTCAGACCCT
GCCATGCAGCGGGTGCTTCACGGTCTCTCAGACCTCCCTGCCTCTTCGGATCTAGATCTAATTTTTCTCCAAGGAATCATGGAGAGTCCAAAGGCTCATG
AATGCATGGAGGAGATCCGTTTGGAAGCCGTGAGGGAAAATAATCTGCAGCTGGTACAGGAGATTCTACAAGACATGGCAGGAGTACGAGACCCAAGTGG
TGTAGCAGCTGAGCTTCAGGGAATCCTTCAGGAACCACACTTTCAGTCCCTCTTACAGACGCATGATTCTGTTGCTTCGAAGAATTATGAGACACCTCCT
CCTAGCCCAATGCTGGACCCCACCCTAAGTAATCAGCCAGTGCCACCTGATGCAGTGCGCATGGTGGGAATAAGAAAGAGCGCCGGGGAGCACCTGGGTG
TGACATTCCGTGTAGAGGGAGGAGAGCTTGTTATTGCCCGCATTCTGCACGGTGGGGTAATTGATCAACAGGGCCTCCTGCACGTAGGGGATGTGATCCG
GGAAGTGAATGGGAGAGAAGTGGGCAGCGACCCACAGGCCTTGCAAGAGATGCTCCGAGAGGCCAGCGGCAGCGTGGTGCTGAAAATACTACCCAGCTAT
CAGGAGCAGCATCCTCTGCGGCAGGTGTTTGTTAAGTGTCACTTTAGTTATGATCCTTCCAGTGACAGTCTGATCCCATGTAAAGAGGCTGGCTTGCCTT
TCAAAGCTGGGGACCTTCTACAGATTGTTAATCAAGAGGATCCCAACTGGTGGCAGGCCTGTCTGGTAAAAGGTGGCACTGCGGGTTTGATCCCCAGTCA
ACTACTGGAGGAAAAGAGGAAAGCATTTGTAAAACGGGATGGAGAGTTCACTCCTAACTCCAGTGCTTTATGTGGAAGTATTGGTGGAAAGAAAAAGAAG
AGGATTATGTATGTAACAACAAAAAATGCAGAGTTTGACCGTCATGAGCTACTGATATATGAGGAGGTTGCACGTATGCCCCCTTTCCGCAGAAAGACTC
TCATCTTAATTGGTGCACAGGGTGTGGGGAGAAGGAGCTTGAAGAACAAACTGCTGACTTCTGACCCTTCAAGATATGGCACCACAATCCCATATACATC
TCGTAAGAGAAAAGAAGGTGAATGGGATGGCCAATCATATTCCTTTGTAACCCGGGCAGAGATGGAGCAGGACATCAAGGCTGGTCGATATTTGGAACAT
GGGGAGTATGAGGGGAACCTCTATGGCACCAAAATCAGTTCGATCCAGGAAGTTGTGGCATCAGGAAAAATGTGTGTGCTGGATGTCAACCCTCAGGCAG
TGAAAGTTCTGAGGACGGCAGAATTTGTACCCTATGTTGTATTTGTTGGGGCTCCTGACTTCCAGACCCTAAAATCCAACAATCTGTCAGCAGTGGAAGT
TGGCATTACAAACAAGCAGCTGACGGATGCTGAGCTTCTGCGCATTGTAGAAGAGAGTGAGCGTCTCCAGCAAGCTTACGGGCATTACTTTGACCTTACT
TTGGTCAACTATGATCTGGATCAGACATTCCAGGAACTGCAGGTGGCATTGGAGAAACTCAACTCAGAGCCACAGTGGGTTCCAGTCAGCTGGGTCTATT
GAACCTCAAAGTTCTCATATTCTCAGTTTAACATATTCAGAGTCACAGAAAGGGCATATATATTATATATTGTTCCTTTACCTACTGATTCACATTTGAA
CAAAGATTTTACATTTTGGTTTATTCTCCCTTCTCTAAGCTATAAAGAGACAAAGTTTTGACTGAAGCTGTAGAACTACAAATCCCAGGTAACGGAAAGG
AGTATGGGATTTGTCATATATATCTATGTTTAATCATGACCACCTTCATATTCATATACATAGAAATGACAAATCCAAACAAGTAAGAGCTTGGCATCTC
TTTGGATGGCAGGGAATCTGAAAAAAATCTGTTATTTTACCCATTAAAGCAATCATTAAGATAGGACAATACTGGCTCAAGAACATGCATTTACAGTGTT
CATGGGGGTGGATTTGCTTTTCTGCTGCAGATGAAGGCAGATTTTTAATGCTTCCCCCTCACTTACCAACATTCAGACTAGGCAAGGAGGCAGGGCTTCA
TTTCCAGTTAAAGGTGAACCATTGCTAGCAGAAAAGTAAATGTCACTAGTGATGGGCAAAATGTTTCACCAGGCATGGATTCGCGGCGAATTTCCGCGCT
TCACCATTGGCGGATTGTTTCGCGAAATGGATGAAAAAATTTGCCACGGAAAAATTTGCTGCACGTCCAAAAATTGTCGCCTGCGTCAAAATAATAGTCA
CGGGCATCAAAATAATAGCCACGGGCGACAAAATAATAACCGTGCGACAAAATAATAGCCGCGGGCGACAAAATAATAGCCGCGCGAGGAAAGAATAGCC
GCTGGCAACAAAAGAATAGCCGTTGGCGACAATATAATAGCCGCGGGCGACAAAATAATAGCCGTGCGACAAAATAATAGCCGCGTGAGGAAAGAATAGC
CGCGGGCGACAAAAGAATAGCCGCGGGCGACAAAATAATAGCTGCGCGACAAAATAATAGCTGCGAGAGGAAAGAATTGCCGCGGTCTACAATTTTTTTT
GACGTGCAAATTTTCCTCCGTTTCGCGAATCTTTTGAAAGATTCGCAAATTTTTCGGCGAAGCGAAACGGGACAGATTCGCTCATCACTAAATGTCACCC
AAATCAAGGTCCTCAATTTGCCTCATGGCAAAAACTCCTCTGAATCTGTACTACATCACAGAGGCAGATAGGTAATTTGTAATCATTTATTTCATGTATT
GAACTAATGAACTAGTAGGAAATACACAAAGGACACAATTTTGCAACAAACCAAAATATTCTTGATGACCATAACTAAGACACCATGCACAAATGTAAAT
ATTACTGTTTTTTCAAGGATGCAAGTTCTGTTGAATAACAGTATATATATATATATATATAGCCCTTGTGTTTAGCCTTATACTGTATAACCCAAAAGCT
TCTAAGGTTGCTGGTTTATGGGCAGATATTAGCAGTTTATTGGATCATGTGCAGAAGCAGAATCTTCATGCTTCCAATCTATATCAACTGGGGCTGGCGG
GAAATTTCCATTGGCCATAGATCACATTAGCCATGTGTGATGTGTGACTGGTGGTATTTCTCAGGGCAGAGGCAAAAAACATCAGACCAGCCATATTGGG
CCCAGCCCATTGAATGAATGGACTTGTTGAGGGTGATCTTATAGAACATTGTATTTATAATTTGCTTAAAGCATAAAGCATACTTGGTTAATTTATTGTT
TAGAGTGAAACACCTAATATTGTTGCAAATGTTGGAAGTGAAGTAATGTTTACATCCTAGATACAATTTGTTATGTATAATATGTGTACTGTGATATTAA
ACGAACACAGGTAAATAATGTGGATTCCGTTTAGATAAGGATGCTGCACACTGGAGAAATGTTAGAGTTATGTATGACTATATGGTTCTATATAGATACG
AGGTAATATTTTGCCATGTATAGTAACCCATTACAATCAATCAGTAGTTAGCATTTACTCTATAGTATGGCCAAAAGTGTATTAATATAAAGGTGCAATT
CCACACAAGGTTGGAATGCTTTACCTGTGAATAACCTTTCCATAGGTAGGGTTTGAGACAGTGAAGTGACCACCTGCCGTAGGACATGTGGTATATCGGT
AGAGCGGGGGACACTGTAGCACACAAAACAGGTAAAGTTAGTGCTTAAAAGGTGTTTTAATGGTTCCATATGTAGATTTACACAAGACTTGTGTATGGAA
CCATTTAGGCAATAACTTCATGTGTTTGGTGTGCTACAGTGTCTCCTGTTCTATCAATATGAAGATGGCCCTTCCTTTGTTGCTATTAAAACCTTTACTC
TTCTGGGAAGGTTCCCACCAGATATTGAAATATTGTTGGTGGAATTTGCTTCCATTTAGCTTCATCACAGCAACTAATGTTGGGTACTAATGTGGGGGAT
TAGACCTAACTCAGCCAATTCATCCCTTGTGTTGGGTTGAGGTTAGGGGTCCCTGTAGGCCAGTCACAGTCCACTCCCATCTCAGCAAACCCATTGTTTA
TGGACCTGGCTTTCTGGACATGGGTATTATTGTGCTGGAAAAGGAATTAATATTTCCCCAAACTGTTTCCACAAGGTTGGGATCATTGCACCCCGTAGCC
TTAAGATTTGCTTTCAGTGCAATGGAGGACCCTTTACAAAACCATAGAACACAGCCCTATACCATTATTCCTTCTCCAGCAAACTTTACTGTTGGCCTTA
TGCATTGAGACAGGTAGAGTTCTCCTGGCATCTGTCAAACCCAGACTTTTTTGTCAGACTATATTTGGTCCTTGAGCTGACATTCCTTCTGGAGACAGTT
TGAAAGTCAACAGTAATTTTTCCAATGGAGGACATGGATTTTTTTTAGATTTAGGGGCAGTTTTATCAGAATGTGAGATTACAGCTCACCACAGAAGGGT
TCACCCACTTTCTATTCATTTATATGGAAGTTTTGGAAGTGTGTTTATCAAACTGTGAAGTCTAACTTTCACTCATTGCTTAATATGTTTCTAGAATCCC
ATAGGAATGAATAGGGAGTGGGTTAATTTTTAAGGGGTGAACTCTAATCTCATATTTTGATAAATCTGCCCCTTAATGTCCAAATACTTTAGACATTAGC
AGGCCTTACATCTGTTACTATATAACCACCTTCAGTACACTAATTGATCAATTAAGTTTCTATTATCTGTATCTGTTCTATTATCTGTTTGTTTAGCCAC
TTTGAAAACCTCAAGGTATATTTTAGTCTATCAAATATCCCTGCCATTGATCTTAAGAACAATCTACCTGTGACCACATCTACAGTACTGACTATTAGCT
GAATAAGAAAACTGAGTGTTCCAGTGTATTTGTGTGAATATTTTAGATTTTATATTTTTGACTGATATATTTAAAGAGATAAAGTATACTCAATTTATTT
GTAGACAGTACTGTATTGTTTCTGTAGAAATGTGAGGTTTGTAGTGTGAATACTTTACTATAGGCTTTTGAGGCCTAAGGTCAGAAAACCCAGTTTGGTT
TTCTATTCACAGCATAACACTGCCAGATATTTGTGATATATTATACATTGACCCGATAATTCCCAGAATGTAATTAGCATTTTCTCCTTGTAGCATTTTG
TGTTGTAAATTTCAGATTTCAATAAAAAGTTATATATCTCTATATATGAAATGAAAAAAAAAAAAAAA
5' EST: CX842098 [+]atcaggacctaggcaccccaggccctttatatcctgccatccagactgagaggggtacctgcggatctttaggaccactcaacatttgtaatattttctt
gctggtacatcctctataccatctattctcttattatccaggaatctcttgggtacccaatgcctgtcgctctgccttcccacaactcagaccctgccat
gcagcgggtgcttcacggtctctcagacctccctgcctcttcggatctagatctaatttttctccaaggaatcatggagagtccaaaggctcatgaatgc
atggaggagatccgtttggaagccgtgagggaaaataatctgcagctggtacaggagattctacaagacatggcaggagtacgagacccaagtggtgtag
cagctgagcttcagggaatccttcaggaaccacactttcagtccctcttacagacgcatgattctgttgcttcgaagaattatgagacacctcctcctag
cccaatgctggaccccaccctaagtaatcagccagtgccacctgatgcagtgcgcatggtgggaataagaaagagcgccggggagcacctgggtgtgaca
ttccgtgtagagggaggagagcttgttattgcccgcattctgcacggtggggtaattgatcaacagggcctcctgcacgtaggggatgtgatccgggaag
tgaatgggagagaagtgngcagcgacccacaggccttgcaagagatgctccgagaggccagcggcagcgtggtgctgaaaatactacccagctatcagga
gcagcatcctctgcggcaggtgtttgttaagtgtcactttagttat
3' EST: CX842097 [+]catatatagagatatataactttttattgaaatctgaaatttacaacacaaaatgctacaaggagaaaatgctaattacattctgggaattatcgggtca
atgtataatatatcacaaatatctggcagtgttatgctgtgaatagaaaaccaaactgggttttctgaccttaggcctcaaaagcctatagtaaagtatt
cacactacaaacctcacatttctacagaaacaatacagtactgtctacaaataaattgagtatactttatctctttaaatatatcagtcaaaaatataaa
atctaaaatattcacacaaatacactggaacactcagttttcttattcagctaatagtcagtactgtagatgtggtcacaggtagattgttcttaagatc
aatggcagggatatttgatagactaaaatataccttgaggttttcaaagtggctaaacaaacagataatagaacagatacagataatagaaacttaattg
atcaattagtgtactgaaggtggttatatagtaacagatgtaaggcctgctaatgtctaaagtatttggacattaaggggcagatttatcaaaatatgag
attagagttcaccccttaaaaattaacccactccctattcattcctatgggattctagaaacatattaagcaatgagtgaaagttagacttcacagtttg
ataaacacacttccaaaacttccatataaatgaatagaaagtgggtgaacccttctgtggtgagctgtaatctcacattctgataaaactgcccctaaat
ctaaaaaaaatccatgtcctccattggaaaaattactgtgactttcaactgtc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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