XB-CLONE-3298939
Clone Name: IMAGE:7661685
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Gene:
st3gal3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121863 [+]
GGCTTCGAGTATCATCTGCTAATTGGTTTGACGGAACCTTGTGGTCAGTTTCCTTCAGACCTCCGCAGACAGCCGGGCAGTACTAGGCAGAACACAAGGG
GGCCCTCCACGTCCAGCCTGCACCACCAGAGCATGGCGGCGCCCCGGGGCCAGTGCATAGCAAAGAGCTCTGTCAGCTATATGAAGGCCTCTCCGGGACC
CTCTGTTACGCAGCGGTGCTGACCACCAACGGGCCCTTGCATGCCGAGCCGGACAGGTAGTAGCACCAAGAAGAGGAGCGAGTCTAGTCCTGGAGATAAC
GGTCCTGCTAGGGGAGGTCATGTAGGATTGCAGGTATGCTGTGAAGATGGGAATTTTGGTGTTCATGAGGAACTTGCTGCTAGCCCTCTGCCTGTTCCTG
GTGTTGGGCTTTCTCTATTACTCTGCCTGGAAGCTGCACCTTTTGCGTTGGGAAGATTCTCATTCAATTCTACACGCCTTTGACTCCGTTGGACACAAAT
TAGGCCCAGGACGGAGAGAGAGAAAAGCGGGGATCCTGGCCCTACCCTCATCTGCTCCATCGCAAATTGCTGCTGACAGCATCCAGCAAGATCTCAATGA
ACCAAGGGTTGCACCAGTGCCATCGATTCTAAAATATGATCGACTGGGCTTTCTACTTAAACTGGAAACTAAACTACCAGTAGAACTGGCAACAAGGTAT
GCAAATTTTTCCGAAGGTGTGTGCAAGCCCGGCTATGCTTCTGCGCTCATGAATGCCATATATCCAAAGTTCTCCAAACCAGCACCAATGTTCTTGGATG
ATTCATTTAGAAAATGGGCAAAGATTCGCGAATATGTTCCTCCCTTTGGTATTAAAGGGCAAGATAACCTAATCAAAGCCATCTTGTCTGCTACAAAAGA
ATACCGACTCAAGCCAGCATTAGACAGCTTAAACTGTCGACGATGTATCATAGTGGGGAATGGAGGTGTTCTTGCTAACAAATCTCTAGGATCTAAAATC
GATGAATATGAAGTTGTTGTCAGGTTGAATTCTGCTCCAGTTAAAGGTTTTGAGAAGGATGTAGGCAGCAAAACAACTCTAAGAATCACATATCCGGAAG
GAGCAATCCAGAAACTTGAACAGTATGAAAAAGACTCCATATTTGTCTTAGCGGGTTTCAAGTGGCAGGATTTTAAATGGCTGAAATATATTGTTTACAA
AGAGAAAGTGAGTGCCGCTGATGGATTTTGGAAGTCAGTAGCCACACGTGTTCCACGTGAACCACATGAGATCCGTATCTTAAATCCATACTTTATTCAA
GAAGCCGCCTTTAGCTTTATTGGGTTGCCATTTAATAATGGTTTGATGGGAAGAGGGAATATCCCAACGCTGGGTAGCGTGGCTATAACTATGGCACTAC
ATAACTGTGATGAGGTGGCAGTCGCAGGATTTGGTTATGACATGAATCTGCCTAATGCTCCACTGCACTATTATGAGACTATCAAGATGGCAGCCATTAA
AGAGTCTTGGACTCATAACATCCAAAAGGAGAAGGAATTCCTAAAGAAGCTCGTTAAAGCACGTGTAATTAATGATCTTACTAATGGCATCTGAGCGAAG
AGGAGTATCATCAGAAGCCGGAAGATGCACTGATTTGGATAAGCATGGATACCCAATGTTACCAGAGCTTATCTTCTGTCCCAAGACAGGCTTGCATGCC
TTCAGATGTCCAACTTCCAAGTGAAAGTTTGGTTGTTCAATTAAGCCAGTGGCCATAAGAAAAGAAAGCAGTACAGAAGTATCTGACAACTTTAAGGGAC
AACTGTTTTTGTAGTAACTACAACTTTAATCCGGAAACAGACATTTTGTTTTAAATGGACAAGTTACTGTGCCAAATGTACCTCTAGTTTGGGATTTGAG
GTGCAAAAGGTACACCACATGCATTTTGAATGTATAAAGTATTATTTTAGATAAAAGCATGGGGTTATTCTGCGTGGGAGACAGAGATATGGATGGAAGG
ACATCATCACACTAATATTTTCATATTCATGCAGATTACAACATATCTCTGGCTGTTTTATGCAGAATCCTATGTAAAACTAGGACCCATGACCGTCCAG
GGTTAGGGGCATTGTTGGTGCTGCTATTACTTAGATGAGCAAAGAATTGTTCAGTCTGTTTGTTATTGAAGAATGTTTTAAAATGTATGATTAAGGGTGG
GAAGGGTTATCTGGCAGATTGTAAACACCAGAATCTTCGATCAGTGTTAATATTTCACTGTGGATCAGTGTTTGAAATCTTAAGAACAGGCACTTTGTGA
CCTGTTGTCCCATTTTTGCCTTTGCATGAATAATGTGAAGGGCGCAGACTTTCTGCACACAGAAATCATGTTCAATTTATCATCTACAAAGGTGTAAGTA
ATTCAGACAGAAACATCAGTAATACATGTCATATCAAGAAAGAAGGCGTTATCCAGAACCAGATTCCACCACCTTTGTGCCAGACACATGAGCAAACAGA
GTGGAATCTTCCATTACAATATCAGATCATCCAAACTAATAGGATTTATCATTAAATTCCCTGATGTGTTGCAGAGACTGCAGTTGATTCTTTGAGAATT
CCTTTTTAATTACTGACAAGCATATCCCCAATTACTGACAAGCATATCCCCCACACATTCTTTAGTTCTTGTCTTTTTATCTTATCTTTTTACATTTGAA
AATCTGAATAACCGTATCTTCAGACTTGATATTTGTGACCTCAGCATTCATTTCTCCTATGGCTACAGTACATATCTGGTCCCAAAACATAGCCAGTAAA
ACATTAGCATACTTGAAATTGCCTTGTTTCTGTTTGTGGTGTTGGCAAAAATAGTGTGGATATGATTAGGCCCATATTTATTTTTGGGACATTATGCAAT
TGTCTTCAAGTTTTCTCTAGGCTGCAATAAATGGCAGATTTATGCAAACAAAAAAAAAAAAAAA
5' EST: CX843178 [+]gcttcgagtatcatctgctaattggtttgacggaaccttgtggtcagtttccttcagacctccgcagacagccgggcagtactaggcagaacacaagggg
gccctccacgtccagcctgcaccaccagagcatggcggcgccccggggccagtgcatagcaaagagctctgtcagctatatgaaggcctctccgggaccc
tctgttacgcagcggtgctgaccaccaacgggcccttgcatgccgagccggacaggtagtagcaccaagaagaggagcgagtctagtcctggagataacg
gtcctgctaggggaggtcatgtaggattgcaggtatgctgtgaagatgggaattttggtgttcatgaggaacttgctgctagccctctgcctgttcctgg
tgttgggctttctctattactctgcctggaagctgcaccttttgcgttgggaagattctcattcaattctacacgcctttgactccgttggacacaaatt
aggcccaggacggagagagagaaaagcggggatcctggccctaccctcatctgctccatcgcaaattgctgctgacagcatccagcaagatctcaatgaa
ccaagggttgcaccagtgccatcgattctaaaatatgatcgactgggctttctacttaaactggaaactaaactaccagtagaactggcaacaaggtatg
caaatttttccgaaggtgtgtgcaagcccggctatgcttctgcgctcatgaatgccatatatccaaagttctcccaaccagcaccaatgttcttggatga
ttcattt
3' EST: CX843177 [+]gcataaatctgccatttattgcagcctagagaaaacttgaagacaattgcataatgtcccaaaaataaatatgggcctaatcatatccacactatttttg
ccaacaccacaaacagaaacaaggcaatttcaagtatgctaatgttttactggctatgttttgggaccagatatgtactgtagccataggagaaatgaat
gctgaggtcacaaatatcaagtctgaagatacggttattcagattttcaaatgtaaaaagataagataaaaagacaagaactaaagaatgtgtgggggat
atgcttgtcagtaattggggatatgcttgtcagtaattaaaaaggaattctcaaagaatcaactgcagtctctgcaacacatcagggaatttaatgataa
atcctattagtttggatgatctgatattgtaatggaagattccactctgtttgctcatgtgtctggcacaaaggtggtggaatctggttctggataacgc
cttctttcttgatatgacatgtattactgatgtttctgtctgaattacttacacctttgtagatgataaattgaacatgatttctgtgtgcagaaagtct
gcgcccttcacattattcatgcaaaggcaaaaatgggacaacaggtcacaaagtgcctgttcttaagatttcaaacactgatccacagtgaaatattaac
actgatcgaagattctggtgtttacaatctgccagataacccttcccacccttaatcatacattttaaaacattcttcaat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: