XB-CLONE-3298651
Clone Name: IMAGE:7662181
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Gene:
cnksr2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136228 [+]
AGAGTGTGAAGAGGCGTGATGTGTCCGGCAGCCTGAGAGCTGATGCTGTGTGTGGGAAGCGCGTACTGAGGGGGACAGGAGACGGCTCGGCATTGGTACA
ACCCACAGCTGCCTGGGAACAAAGCGGGCTCTTGTACTGCTGCCAAGTGGCCACCCTGCTGCCCGCCAGGCTCAGCCAAGGGGAACATATTCCGCTCTCT
TACACACTTTATTCATTCTGCTTCTCCCGTATCCGCTATTCCTGGCGCTGGGAAGAAGATCGACTAAAGTTTGGCAATGGCTTTGATCATGGAACCCGTG
AGTAAATGGACTCCGAGTCAAGTGGTGGACTGGATGAAGGGCTTGGATGATTGCCTGCAGCAGTACATAAAGAACTTTGAGAGGGAAAAGATTGCAGGGG
ACCAGTTATTACGCATTACACATCAAGAGCTTGAGGAATTAGGAGTCACACGGATTGGACACCAGGAACTTATTTTGGAAGCTGTAGATTTACTGTGTGC
ACTGAATTATGGATTGGAAACTGAGAACTTAAAAACACTTTCCCACAAATTAAATTCATCTGCAAAAAATTTGCAGAACTTTATAACAGGGAGAAGAAGA
AGTGGCCATTATGATGGGAAAAGTAGCAGAAAGTTACCTAATGACTTCTTAACTTCTGTTGTGGATCTCATTGGGTCAGCAAAGAGCCTCCTCGCTTGGT
TGGACAGGTATTGCCACGCTGATCAGGATGACATCGATAACCTTAAAAAAAGATGGATAGGGCATAAACTGGATAAATCACCATTTGCTGCAGTGACAGA
TTATTCAGTCACAAGAAATAATGTTATTCAGCTGTGCTTGGAATTAACAACCATTGTTCAGCAGGAATGTACGGTGTATGAAACAGAAAACAAAATTCTT
CATGTGTGCAAAACCCTCTCCGGTGTCTGTGATCACATCATCTCTCTCTCATCGGACCCGCTGGTTTCACAGTCAGCTCATCTGGAAGTCATCCAACTCT
CCAACATCAAGCCAAGTGAAGGACTGGGTATGTACATCAAATCCACATATGATGGACTTCATGTGATTACTGGGACTACAGAAAATTCACCTGCTGATCG
ATGCAAGAAAATTCATGCTGGTGATGAAGTAATACAAGTCAACCATCAGACTGTGGTGGGTTGGCAGTTGAAAAATTTGGTGAATGCACTACGAGAGGAC
CCGAGTGGTGTTATCTTAACTTTGAAAAAGCGACCTCAGAGCATGCTTACCTCAGCACCAGCTTTACTGAAAAATATGAGATGGAAGCCTCTTGCTCTGC
AGTGGTATGATGAGGAAATGAAAACTTATTCCTGGACACGTATATCACCCCTCATTCCAAGGAGTCCCACCAGCGGTGCCGCTACTCCATCAAGCACAAT
CAGCACACCGACAAAGAGAGACAGTTCAGCACTTCAGGACCTCTTTATTCCTCCTCCACCCCCAGAGCCTTACATACCCAGAGATGAGAAGGGCAACCTA
CCTTGTGAGGATATGACTAGACACACTGTTGGCAAACCTGTTTCGAAGGGATCAGAGTCTCCAAACTCCTTTTTAGACCAGGAGTACAGAAAGCATTTCA
ATGTGGTGGAAGAGGATGCAATTCTGTATTGCTACGAGTATGACAAAGGAAGATCCGGCATGCAAGGTCGGAGGGAAAGCACGCCAACCTATGGAAAACT
GCGTCCAATTTCTATGCCCGTGGAATATAATTGGGTTGGGGATTACGAAGATCCCAACAAGATCAAGAGAGACAGTAGAAGAGAAAGTTCATTGCTACGT
TATATTAGCAATGACAAAGTAATCCAAGAAGAATACATGCTTCAGAGGGGCACAAAAAAAGAAACTGGTAAAAAATCCAAGAAGAAAGGAGAGAAGAATA
CAAGTCCAAGCCATTATGCCCTGATCCCTGCTGTACAAATGGATTCCCTGTGCCATGAAGTTGTGAGCCCATCTGTTTCAGACGCTGCATCATATCATAC
ATTTCAGCAATCTTCATTACAGCACAAATCAAAGAAAAGGAATAAAGGTCCTGTTCCTGGCAAAAGTAAAAGGCGAATATCATGTAAGGACCTGGGTCGT
GGCGATTGTGAGGGATGGCTATGGAAGAAAAAAGATGCCAAGAGCTATTTCTCTCAGAAATGGAAAAAGTACTGGTTTATTCTGAAGGACACATCTCTTT
ACTGGTATATAAATGAGGAGGAAGAGAAAGCTGAAGGCTTCATCAGTCTGCCTGAATTTAAAATTGATCGAGCCAGTGAATGCCGTAAAAAATTTGCATT
TAAAGCCTGCCATCCCAAGATCAAGAGCTTCTATTTTGCTGCTGACAACTTAGATGATATGAACAGATGGCTTAGTAGAGTGAACCTAGCAGTGTCTAGC
TATGCAGAGAGAGAGAGAGGATTAAACAGGATCAAGGCAGCATAAATTATTGGAGTGAGAGTGATCAGGAAGAAGTTGATACACCATCAACACCAAAACA
AGACAGTCCGCCGCCTCCATATGACACATACCCAAGGCCAGCCTCCATGAGTTGTGCCAGCCCATTTGTGGAACCAAAACACAGGCTGTCTTCCACTGAA
ACCTCACAGTCCCATTCCTCGCATGAAGAATTTCGGCAGGAAGCAGCTGGAAGCACTACAGATTCTCCAGTAAGAAAAACAGCCAGTCAAAGGCGATCCT
GGCAGGATTTAATTGAGACACCACTAACTAGCTCAGGACTTCATTATCTTCAGACTCTTCCTCTAGAGGACTCTGTATTTTTGGAGCCAGCTGTGGTTTC
GCCAGATCACAGACGACAGTCAACCCTTCCAGCTCCAAAGTGCCATCTTCTAGATCATTATGGTCCTTTTTCTTTGGCAGAGTGTAAACAATCACAAGCA
CAAAGTGGTAGTGGGGCAAAACCACGCAGTTTTACACTCCCACGAGATGGAGGCTTGAGCCACTGTGCGCCACTGTCACCAGTGAGTATCCTGGAGCCAC
TGGAATCCTCACCGAAACTGGAAACACAAGGTGAAGCAAAATCTGACCAACCAGGGAAAGTTGAAGATGCAAGCCAAAGAAGGAGAAGTAGCTATTATTG
ACAAACTTCTGGAAAAACCAGACTTCACATCTAAAGACTTTCAGGAGTGGAAGAGCCTGTACCTTGAGCTTTTTCTGGAAATCTGTCAAAAGAGTAACTC
AAAGGACGGGGAGAGCGTCTCTTCAGAAGTAGATGCACTTATACAGTCTCTAGCGCACATGCACTCCTATATCGAAACGCATGTATAATCGTCTTTATTC
TCTGATGCCTATAACCCTGAAGAAAGGACATTTTCTATTATTATATGGAAAGTCTAGTAAATTATATTTTAAAGTTGCTGATCTATGTGGAGTAACTCTT
CTATGATCCCAATGCCGTCCTCTTCGATTGGCGTTTGTATATTTTCTACGCTGGTAGTGCTTAGCTTTATGTTCATCCTGTGATTCGGTCGGTAAAGTGT
ACAGAGATGATTTATTAAACTCAGCGATGGAAGCGGTTTTCTTAAGGCCTCATCCCTTGGAAAACTTTTCTATTAACAGATTTACATTTTTTCTGTGCAA
ACGGGATCGGGGCGACCATGAAAAATAGGTTTTGTATTATTCTTGAAATGTTTGAAAAAAACAAAAAAAAAAAAAAA
5' EST: CX843647 [+]agagtgtgaagaggcgtgatgtgtccggcagcctgagagctgatgctgtgtgtgggaagcgcgtactgagggggacaggagacggctcggcattggtaca
acccacagctgcctgggaacaaagcgggctcttgtactgctgccaagtggccaccctgctgcccgccaggctcagccaaggggaacatattccgctctct
tacacactttattcattctgcttctcccgtatccgctattcctggcgctgggaagaagatcgactaaagtttggcaatggctttgatcatggaacccgtg
agtaaatggactccgagtcaagtggtggactggatgaagggcttggatgattgcctgcagcagtacataaagaactttgagagggaaaagattgcagggg
accagttattacgcattacacatcaagagcttgaggaattaggagtcacacggattggacaccaggaacttattttggaagctgtagatttactgtgtgc
actgaattatggattggaaactgagaacttaaaaacactttcccacaaattaaattcatctgcaaaaaatttgcagaactttataacagggagaagaaga
agtggccattatgatgggaaaagtagcagaaagttacctaatgacttcttaacttctgttgtggatctcattgggtcagcaaagagcctcctcgcttggt
tggacaggtattgccacgctgatcaggatgacatcgataaccttaaaaaaagatggatagggcataaactggataaatcaccatttgctgcagtgacaga
ttattcagtcacaagaaataatggtattcagctgtgcttgg
3' EST: CX843646 [+]caaacatttcaagaataatacaaaacctatttttcatggtcgccccgatcccgtttgcacagaaaaaatgtaaatctgttaatagaaaagttttccaagg
gatgaggccttaagaaaaccgcttccatcgctgagtttaataaatcatctctgtacactttaccgaccgaatcacaggatgaacataaagctaagcacta
ccagcgtagaaaatatacaaacgccaatcgaagaggacggcattgggatcatagaagagttactccacatagatcagcaactttaaaatataatttacta
gactttccatataataatagaaaatgtcctttcttcagggttataggcatcagagaataaagacgattatacatgcgtttcgatataggagtgcatgtgc
gctagagactgtataagtgcatctacttctgaagagacgctctccccgtcctttgagttactcttttgacagatttccagaaaaagctcaaggtacaggc
tcttccactcctgaaagtctttagatgtgaagtctggtttttccagaagtttgtcaataatagctacttctccttctttggcttgcatcttcaactttcc
ctggttggtcagattttgcttcaccttgtgtttccagtttcggtgaggattccagtggctccaggatactcactggtgacagtggcgcacagtggctcaa
gcctccatctcgtgggagtgtaaaactgcgtggttttgccccactaccactttgtgcttgtgattgtttacactctgccaaagaaaaaggaccataatga
tctag
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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