XB-CLONE-3298611
Clone Name: IMAGE:7661951
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Gene:
cib2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121478 [+]
CTAGAAAGCGCTCCTGCCCCTCCCTGTGTGATGCATTTCAGGAGAACCAGGATCCCTCATCTATCATGGGCAATAAGCAAACAATATTTACTGATGAACA
GCTAGATGCATACCAGGACTGCACCTTCTTTACACGAAAAGAAATTCTCCGGTTACACGGGAGATTCTATGAGCTGGCACCTAACATTGTGCCGATGGAT
TATACAGATGACCCAGATGTCAAACTGCCAATGCAGCTAATAATTAACATGCCAGAGCTTGTGGAAAATCCTTTTAAGGAGAGAATTGTGCAGTCTTTCT
CTGAAGATGGAGAAGGAAACCTCAGTTTCAATGACTTTGTGGATATGTTCTCTGTGATGAGTGAAATGGCTCCCCGGGAGCTTAAGGCTATCTACGCATT
TAAAATTTATGATTTCAACACTGACAACTTCATTTGTAAATCAGATCTGGAGAAAACCCTGAACAAACTGACAAGAGAAGAGCTCGAGGAAGAGGAGGTG
AATCTGGTCTGTGAGAAGGTTATTGAAGAAGCTGATATGGATGGGGACGGCAAACTGGCATTTGCAGATTTTGAGAACATGATTTCCAAAGCTCCAGATT
TTCTCAGTACTTTTCATATACGAATATGAAGATTTAGCAATTGGAAGGTTTTGCTGGTTCATAACTGCTGAATCTCAGGCATCTCCAGGCCCTGGCCTCT
CTTACTGTGCAAATACATAAACAAAAGAGCTCCATCTGCTGAGATGAAGCTGACCTTGCATCAGGAATCTTTTTCCGAAATTTGAGGATGCGTTTTCTTG
CTGTCTTTTTTATTCCTTCAATTTTTATTAATTATATTGATGCAAAAAAAGAAGAACAGAAGATCTAGAATTTGAACAGAAGGGGATTATAGGCCTTATT
TGCTGAATGGATAAAGAAGTGGAAAATAGGAAATGACAATATGAACACATCTAAGTGCAGTCTTGCTGCAGAAGAAAACATGCAGCCGTGTGACATCACA
TTTAATGCAATCTTCATTTAACAGGATTCCTTTTTTTCCTGGTAAGTATAATTAGCACCAGTGATTTGGAACAAGTATTTTTTCCAAAGGGCTTTTTCTT
GACGAAGTATAGCATGTCCTGCAAGAATTCAGATTTAAAATCCATTTTAGGAAAGCACAGGAAGCCATATTTTGTCAATTGAGATTAATGTTCTCTCTCT
ATATATATATATGTACCTATACCACAAAAAACATGAGAATATGTGGTGCTTTGTGCTATCTTCTCTTTTCTATAATACTTCGGAGCCCTTGTCTTTCATC
ACTGACAATTTTACAGACAAGCTGGAACGTATGAAAACATTAAGAAATAGGTATGCAAATGACTTGATAGCTGTTATGAACTCCAACTCCACTCCAATAT
TTTAGTGGCTTTTGAGCACAGTGTGTGCATTTTTCAGCAAGGCAATACAAATCTGTTCACAGTTTAATGGAATTAAGTGGAAGTTTAATATGTACAAGAA
AGGTTATAAGGGCTAGAATGGGCGACAGAAGCTCACTCCAAACTAGGGGCACAATGAGGATAAAAAGCACTGGAACGTGATACATAAGTGGACTTTTTAA
TGACTGTGCTTTTGTAGCTTTGTAGCTTCTCACATAAAAACTACTATGGTGCCTTTCATATCAAAGCTGCATTTATGTATATCTCCACTAGAACTTCCCT
TTGATCCATTGGTCCATTTATGGAGCAATTAAAGAGGAAAAAAAATGCTGTTATCACACTTTTCTACAATGTTTCATTAAGTTCTTAAGTTCTAACCAAT
GTGACAGAAGGTAGAGGGTTTCCAGAGTATTTCTGTGGCAGCTGTCACAGTGTGTATTTTATTTGAAATGTTTTTCATTTGAAACAGGGGTGGGCCATGC
CCTAGGCAACCTGGTCGGCCACCTCGCCCCTGCACCTCCCCTCCTTGTGCTTGTGCTGTGCCCCCACCTTGTAATGACAACCAGTGGGGGCAAGAGAAGG
AAGAGCGTCTAGGGGTAGGCAGGAGAGGTTTCTGCCTGGCGCCCCCCAATCACAGCGCCCTAGGCAGGTGCCTCTTCTGCCTACCGCTAGTTCCAGCCCT
GATTTGAAAATTATTCTGTGACTTGCTGCAGTCCCATGTAGCATCCACCTGTTCATTACCTGCTATCCTCTTTGCTTAGTATTGCATCCAGTAGTAAAAC
TTGTGCATTGGTTGGAAAGGAAAATTAAATACAACATCACTATTGGATATATAACTGCCTTAAAGTAATATTTACAAGGGGCTTCTGCAGAGGGAATACA
GATGGTGTCAATCCAGGGAAGAAAGAACTGCTGCCTAGCAGTGACCTCCTTGTGTGGTATTATCTTTTGTCCCCTTGTGAAATGAGATTGATAAAAAAGC
CTTGATTTTTTTTTGGAAAAAAAGCTTTGCTTCCCCCCAAAATTTTTTATTTTGATACAGTGCAATTAAATGATCATGGACAGAAATGTTCTGTCACCAC
ACCACAATAATGTTGGATTTCTTCATTGAGTGGTCCTCAAGGCTGACAGTAGCATACATTGCTAAACGTACACAAAAGAAAATATGTTTTGATGCAACTT
CTAATTTTAATTTAGGAAATGTAATTGAATATAAAAATCTCTTCCTACAAGGACACCAAAGACAAATTAAAAGCAAGAAAAATACATGTATATTCTGTGT
GATTCCCCCCTTTCTTCTGTAGAAATGTCTTTAAAATGATGTATAGGTTAATAAAAAAAAATATTTTTAAAAAAAAAAAAAAA
5' EST: CX843708 [+]ctagaaagcgctcctgcccctccctgtgtgatgcatttcaggagaaccaggatccctcatctatcatgggcaataagcaaacaatatttactgatgaaca
gctagatgcataccaggactgcaccttctttacacgaaaagaaattctccggttacacgggagattctatgagctggcacctaacattgtgccgatggat
tatacagatgacccagatgtcaaactgccaatgcagctaataattaacatgccagagcttgtggaaaatccttttaaggagagaattgtgcagtctttct
ctgaagatggagaaggaaacctcagtttcaatgactttgtggatatgttctctgtgatgagtgaaatggctccccgggagcttaaggctatctacgcatt
taaaatttatgatttcaacactgacaacttcatttgtaaatcagatctggagaaaaccctgaacaaactgacaagagaagagctcgaggaagaggaggtg
aatctggtctgtgagaaggttattgaagaagctgatatggatggggacggcaaactggcatttgcagattttgagaacatgatttccaaagctccagatt
ttctcagtacttttcatatacgaatatgaagatttagcaattggaaggttttgctggttcataactgctgaatctcaggcatctccaggccctggcctct
cttactgtgcaaatacataaacaaaagagctccatctgctgagatgaagctgaccttgcatcaggaatctttttcc
3' EST: CX843707 [+]tttttttttttttttaaaaatatttttttttattaacctatacatcattttaaagacatttctacagaagaaaggggggaatcacacagaatatacatgt
atttttcttgcttttaatttgtctttggtgtccttgtaggaagagatttttatattcaattacatttcctaaattaaaattagaagttgcatcaaaacat
attttcttttgtgtacgtttagcaatgtatgctactgtcagccttgaggaccactcaatgaagaaatccaacattattgtggtgtggtgacagaacattt
ctgtccatgatcatttaattgcactgtatcaaaataaaaaattttggggggaagcaaagctttttttccaaaaaaaaatcaaggcttttttatcaatctc
atttcacaaggggacaaaagataataccacacaaggaggtcactgctaggcagcagttctttcttccctggattgacaccatctgtattccctctgcaga
agccccttgtaaatattactttaaggcagttatatatccaatagtgatgttgtatttaattttcctttccaaccaatgcacaagttttactactggatgc
aatactaagcaaagaggatagcaggtaatgaacaggtggatgctacatgggactgcagcaagtcacagaataattttcaaatcagggctggaactagcgg
taggcagaagaggcacctgcctagggcgctgtgattggggggcgccaggcagaaacctctcctgcctacccctagacgctcttccttctctt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: