XB-CLONE-3297832
Clone Name: IMAGE:7662550
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Gene:
necab1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136090 [+]
GAGACTGAGCCTTTCCAGTGCTGACCTGTGCCTCATCCAAGAGCACAAAGCACATTCCTGGCAGCAGCATCCACATTCCAGAACACAGGCAAGAAGCTCT
GAGCCAGCAGCCAGCCATGGATGGTCAGGAGATGCCTTCTCCTTCCAGGACCGACTCCTCCGAGGAGATCAGCTCATCCCTGCACCTAACCAAGGGCATG
TCCATCTTCCTGGATATTCTGAGGAGAGCAGATAAAAATGATGATGGCAAGCTCTCCTTTGAAGAATTCAAGGCTTACTTTTCTGATGGCATTCTCAGTG
CTGATGAATTAAGTGAGCTTTTCCGCACTATTGATACCCACAAAACTGACAACCTGGATACAGAAGAACTATACGATTATTTTTCACAGCACCTGGGAGA
ATATGAAAGTGTCCTGGCAGCCCTGGAAGACCTAAACCTCTCAATCCTGAAGGCAATGGATAAAACAAAGAATCAATATCAAAACGCCTCAAAACTGGAA
CAGTTTGTGACACGTTTTTTGTTGAAGGAGACACTCAACCAGCTGCAGTCTCTTCAGAATTCCTTAGAGTGTGCCATGGAAACGACTGAGGAGCAAACGC
GATTAGAAAGACAAATTCCAGCAAAGCCAGAGATGCTCGCAATTCCATGGGGGGGAAAACGATCCAATCGGAGGCTTCAGAGGAACAATAGTTTTTCCCC
AAACAGTCCCCAATTTAACATGGGCTTGTTAGAGGAAGATAACCAGTGGATGACCCAGATAAACAGACTCCAGAAACTGATTGACAGACTGGAGAAGAAG
GAACTTAAGATGGAGCCCCTAGAAGAAGAGGTAATCGAGGGGAACACAACATCACATATAATGATTGTTCAGCGCCAATTATCTGTGCTAGCGGAGGAAG
GAGAAGAATTCCGCCTGGCACTCAAGCAGTATGTGGAGTCCGTTTCGGCTCAGACGGGATGTTTACACGTTGGAGTGCAGAAGTTGTCGAATGAGTCGCG
TTTCATCGTTTATGAGTTTTGGGAAAACAGTAACATTTGGAAGAATCACCTTCAGACAAATTACAGCAAGACTTTTCAGAGGAGTAATGTGGACTTTTTG
GAAACTCCAGAAGTCATGACAACCATGCTGGTACCCGCTTCATGGTGGGTCCTCAACAACAACTAGACCTCCAGACCTGCGGTGATGGTGGCAGCAAATA
TCCAATTCATTCTTAAGAGACCAGTACAGCTATATATGTACCGTTGTGTGTCTGGTGAACATTAAGTATGTTCTACTCTCTATACTCCTATCTCCTGTAT
GACTTGCTATACGTAATATCATACATGAACCCGTGATTGTGGGAAAGCAGTCTATAACAGGCTGAGGACCTCATTAGGTGCAGGACACCTGCATTGCACA
AGATTCATTAGCTCTAGAAACAGCAGCACACAAACATGGCTTTTCTGTTGGACCACATCCACAGATGCCTTCCTTCCTAATGTACATTTAGTTTTTCTAG
TGAGTCTGTTTTGTGTATATATAAGTTTTTTTTTTTTTCCATTACAGCATTACATATAGGCGATTTAATGGTCCACAGCCATATAAATGTAGGTCTACAA
GTGCAATAACTTGGGCACCTTGATAAATAACAAACCGGATAATTGCACCAAAACATTCTTTGTATTCATTTATTGCATCCAAACATTCTTTTTTCCCTGG
GGAGACAGTGCCACTTGTTTACATTCACAGAAATTATTACTTTGTAGATTTGCACAGTAGCGTCACAATAACCCCTCTCCAGTACACCAATGGTGCCCCA
TGCCATCTTGTACTTCATATTATTCTTTCCCTCCCCACTTAGGAAGGATGGAGGTAGTGTACTTAACTCCTCTCCTTATGACAGTGCCCCTCCCAGGCCA
CAAGAGAAGATGGCAAACAGAGTTGTTAATACATCATTCCTTGTGTCTCTCCACTGGACAGAATCTCCATTATACCCACTCTAAGGTTGGGGCATGAAGC
AGATTACTTCCTTGTACTTGCCCCAAGTTAGAATACTTGTTACAAGGGAAGCTAGAGCAGGAGTTGCCCCCCAGAACCAGTTGGACTGGTTAAAACCAGC
TCAGCCACAGCCAAGGAATATATAGACTTGTATCTGCCCAAACCAAAAGTGTATGCCTTTTACATAGGAAGCTAGGGCAAAGGAACAGTCACCAAAAGTT
CTCGGCCTGGGTCTTTTTGTTTTGGTCACTAAAGACACCATGTTCAACTACACCCTTGGGTTTTATCCATTTTTAAGCAATTTCATGATCAGTTTCACCT
TTCCATGCATGCCACTCACTAGTCAGTCCCTTGAACATTCTTGAAAAGTAACCAGCCTTCTAAAATAGTGCACTCAAATGACCTTTCTAGATGACTTTAC
AGTAAGTGAACTTTTTTTAGGCAATAAATGGAAAATGGTCAACAAACTTTTTCTTGTAGTGTTAGATTTGTGCTGTATTTTAGCTATCAGTTTTAGGGAC
TGGTTAGTATCAAATAATATCTCCTGTATGAGTAGTAATGTAAGAATACTGTTGGTTACATAAAGATTTCTGTGCATCGATCTGCATTTTTATATGTGTA
TACTCTGTACATGTTTTTCATGTGAATACTTTGCGTATAACTATATCTATGGCACAGCATACAATTATTGTTTAAAATGAGATATGTATTCAAGTAACTG
GGACTGGTGACCCTGACCTGATGGTGTCTCATGAACAGGAAATACATGCCAATTATCATTTACTGAATTAAAAAAATATCTAGCAAAAAAAAAAAAAAA
5' EST: CX844958 [+]actgagcctttccagtgctgacctgtgcctcatccaagagcacaaagcacattcctggcagcagcatccacattccagaacacaggcaagaagctctgag
ccagcagccagccatggatggtcaggagatgccttctccttccaggaccgactcctccgaggagatcagctcatccctgcacctaaccaagggcatgtcc
atcttcctggatattctgaggagagcagataaaaatgatgatggcaagctctcctttgaagaattcaaggcttacttttctgatggcattctcagtgctg
atgaattaagtgagcttttccgcactattgatacccacaaaactgacaacctggatacagaagaactatacgattatttttcacagcacctgggagaata
tgaaagtgtcctggcagccctggaagacctaaacctctcaatcctgaaggcaatggataaaacaaagaatcaatatcaaaacgcctcaaaactggaacag
tttgtgacacgttttttgttgaaggagacactcaaccagctgcagtctcttcagaattccttagagtgtgccatggaaacgactgaggagcaaacgcgat
tagaaagacaaattccagcaaagccagagatgctcgcaattccatgggggggaaaacgatccaatcggaggcttcagaggaacaatagtttttcccaaac
agtccccaatttaacatgggcttgttagaggaagataaccagtggatgacccagataaacagactccagaaactgattgacagactgga
3' EST: CX844957 [+]tttttttttttttttgctagatatttttttaattcagtaaatgataattggcatgtatttcctgttcatgagacaccatcaggtcagggtcaccagtccc
agttacttgaatacatatctcattttaaacaataattgtatgctgtgccatagatatagttatacgcaaagtattcacatgaaaaacatgtacagagtat
acacatataaaaatgcagatcgatgcacagaaatctttatgtaaccaacagtattcttacattactactcatacaggagatattatttgatactaaccag
tccctaaaactgatagctaaaatacagcacaaatctaacactacaagaaaaagtttgttgaccattttccatttattgcctaaaaaaagttcacttactg
taaagtcatctagaaaggtcatttgagtgcactattttagaaggctggttacttttcaagaatgttcaagggactgactagtgagtggcatgcatggaaa
ggtgaaactgatcatgaaattgcttaaaaatggataaaacccaagggtgtagttgaacatggtgtctttagtgaccaaaacaaaaagacccaggccgaga
acttttggtgactgttcctttgccctagcttcctatgtaaaaggcatacacttttggtttgggcagatacaagtctatatattccttggctgtggctgag
ctggttttaccagtccaactggttctggggggcaactcctgctctagcttcccttgtaacaagtattctaacttggggcaagtac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: