XB-CLONE-3297472
Clone Name: IMAGE:7662768
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Gene:
lrrc7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC122962 [+]
GGGCATCCCATGCTCGCACCTGAAGGGCATCTTTTAGGCACCATGAGCTGCTGCCGCCGCTGCCATTGATTCAGTTCCTTCCCGTCCCGCACCCCGGAGA
CCCTAGGAATAGCGCCCGCAAAGCTTCGCTGGAGCCTAATGGGCAATTTATCGTCCCTCCAATTGCAGTGCCTGGAGATGACAACTAAGCGGAAAATTAT
TGGGAGGCTGGTCCCATGCAGGTGTTTTCGGGGTGAAGAGGAAATTATATCGGTGTTGGATTATTCCCACTGCAGCCTTCAGCAGGTGCCAAAGGAGGTG
TTCAGTTTGGAAAGAACATTAGAGGAGCTTTATCTTGATGCTAATCAAATTGAAGAGTTACCTAAGCAATTGTTCAGTTGCCAGGCCCTTCGGAAGCTGA
GTATACAAGACAATGATCTTTCAAACCTGCCAACCACTATTGCAAGTTTAGTGAATCTCAAAGAGTTGGATATCAGCAAGAATGGAATTCAAGAATTCCC
AGAAAACATCAAATGCTGCAAATGCTTAACCATAGTTGAAGCCAGCGTGAACCCCATTTCAAAGCTCCCAGATGGTTTTACTCAGCTTCTTAACTTGACT
CAGCTGTACCTTAATGATGCCTTTCTTGAGTATCTTCCTGCTAACTTTGGAAGGCTCAGCAAATTGCGGATATTGGAATTAAGAGAAAACCATTTGAAAA
CATTACCAAAGTCTATGAGTAAACTTGCCCAGCTGGAGAGGCTTGATCTAGGCAATAATGAGTTTACAGAACTGCCAGAAGGCCTGGAACTAATACAGAA
CTTAAAGGAATTATGGATAGACAACAATTCCCTTCAAACACTGCCTGGGGCTACAGGAAAGTTAAAGCAGTTAATATACCTGGACATGTCAAAGAACCGC
ATTGAAAGTGTAGACACAGATATATCTGGGTGCGAATCCCTTGAAGATCTCTTACTTTCCTCCAATTTATTACAACAGTTACCTGATTCTATAGGTCTCT
TGAAAAAACTTACAAATCTGAAAGTAGATGACAATCAACTTGTAGCATTACCAAATACAATTGGAAATTTATCTTTATTAGAAGAGTTTGATTGCAGCTG
TAACGAGCTGGAGTCTTTGCCGCCCACTATAGGATACCTGCATAGTCTCCGAACATTAGCAGTTGATGAAAATTTCCTCACAGAGTTGCCCAGGGAAATT
GGGAACTGCAAGAATGTCACCGTAATGTCACTTCGGACTAATAAGCTGGAGTTCCTTCCTGAAGAGATTGGACAGATGCAAAAATTGAGAGTGTTAAACT
TGAGCGATAACAGGCTGAAGAATTTGCCATTCAGTTTCACTAAACTTAAAGAACTTGCTGCTCTGTGGCTTTCTGACAATCAGTCCAAAGCACTCATTCC
TCTTCAAACTGAAGCTCACCCGGATACAAAACAAAGGGTATTAACAAATTATATGTTCCCGCAGCAACCGCGTGGTGATGAAGATTTCCAGTCAGACAGT
GACAGCTTTAACCCCACACTTTGGGAGGAACAGCGGCAGCAACGAATGACAGTCGCATTTGATTTTGAAGAAAAGAAAGAAGAAGAAGAAGCCCCAGGAA
AACTCAAAGTGGAAATTAATCTAAAACGCTACCCAACACCATATCCTGAAGATTTAAAAAATATGGTCAAATCGGTGCAAAACTTGGTAGTCAAAGGAAG
CCTTGGATCATCATTGGAAAATTCCATACCAACACCAAGTGCTGAACAAAATCTCAAGGAAAAATATGAGCATAAGTGGCCAGTATCAACAAAGGAAATG
AAGTCTGAGGTGTCAAATCGGGAACATAAGGAGTTCCAAAAAAGCCAAATATCAGACTCAGGCAATATTCGTAGCTTTTCTGTGGATGTACCTAAAAGGA
AGGAAAAGGAGGAACATTCAGAGAGACCAGAGCAGGAAGCTTTTGTGCATCGTCCAAATGAGATGAGAATAGCAGAGCTTCGTCCTTCCTTAATTGAAAC
TCCATTATACCCACCCAAACTGGTATTGTTGGGAAAAGATAAAAAAGAGTCTACAGATGAGTCTGAGGCTGATAAACATCATCATAACAGCGTTTCTTCA
GGCACCTACTCAGACTATTCTCCTTCTCAAGCATCTTCGGGATCATCTAATGCTCGGGTCAAAGTGCAGATTTCAGCTAAGGAAGCAGTAAATAGTTCTT
TGTGGGGTAACAGACTTACCCAGTCATTCCCACAGCCACTTGAAACAAAGCCATTACTGAGTCAGCGAGAGTCAGCACCGGTTGGGAATTTACAACAGCG
AAATGATAGACAAGCTCTCAATGACTCCTTTACTGAGAACTGGAATGATAGCCCACACTATGATAACACTGGTTTTGTTGCAGAAGAAAATGTAGTAGAG
AATCCAAGCAGTAACCCAATGCTAAATGCAAAATCAAGAAGCACATCTGCTCATGGACGCAGGCCATTAATTAGACAAGACAGAATAGTGGGTATTCCTT
TGGAACTGGAACAGCCTGCACAAAGAAATTCCCAAGAATCAGATGTGGCCAATCCTTGGCAGAACTGGACTAGAACTCCAAGTCCTTTTGAAGACAGGAC
TGCCTTTCCTTCTAAACTAGAAAGCACTCCTGTCAGTAGTCCATTGCCAGAAAGGAAGGAACATAAACAGCAGTCTCTTGAAACCCCAAGCACATATTCA
TCAGGGGCAACCTGGGAGTTTCATGATTCAAGTACCAGTAGGAGTATGGGGAATGTATTTTCACATAACCATTGTCGTCCAGAGTCTGCCAAAAACTCTG
TATCAGTGAGCAAAAGTACTGAGAGGCTGTCACCATTAATGAAAGATTTAAAGACTGGCCGGTTTAAAAAGTCACAGAGCATAGATGAAATAGATATAGG
TGCAATGAAAGTGTATAACATACCACTGCAACATTATGACCCAAGAAATGTCATGCAAGGAAGTCATGAAAGACAAGACTTAGGGATGGTGCAGGAGCAG
AGTATGTCACGCAGTCAGTCAGTACCAATGCTTGATGATGAACTGCTTGGTTACGGAAGCAGCAGGGGGCATCAGCAAAAACATGTAACTAAAAAAGTGT
ATCAGTTTGACCAAAGTTTTAACCCTCAAGGAGCAGTAGAGGTGAAGCCAGAAAAAAGAGTACCTGCACCTTTTTCATACAATCAAGAATATGCTGCACA
GACAAAAACAATTGCAAAGGATTTGATCAGCCCAAGGGGCTACAGAGGTTATCCCCCTATGGAGCAGATCTTTTCTTTTTCTCAGCCATCTGTCAATGAA
GATTCAGTTCCACCCCAATTTACAAGTCAAGGGTCAAGGCCAGGGTTTTTGAAAAGAGCAGATTCATTAGCAAGTGCCACTGAAATGGCAATGTATAGGA
GAGTCAGTGAGCCGCATGAACCTCCACAGACTGATAGATATGGCAGGCCTCCTTTTCTTGGGGCTATGGACCGCCAAAGTAGCATGTCAGTAGCTGAGTC
ACAGTTTCTGAAAAGGAATGGAAGGTATGAAGATGATCATAGCTTATACCCAGAAATGAAATCTCAAAGTGGTAGTTATCAAGTTAAAACCCTTACACAA
AGAAGGCCATTATCTGCAAGAAGCTACAGTACAGAGACCTATGGAACCACTCAAACAAGACCTGTTTCTGCAAGGCCCACAATGGCAGCTCTGCTTGAAA
AGATTCCATCTGATTATAATCTGGTTAACTATGGTGAAAAATCATTTGAAAACAGTGATATAAAGTCAAGGCAAAATGCTGGAAAATTAGAAGAAAGCTG
CAATAAGATGCCTGCAGACTGGAGACAACAACTCCTTAGACATATAGAAGCTAGAAGGTTAGACAGGACGCCATCACAGCAAAGCAACATTTTAGACAAT
GGACAGGAAGACATGTCTTTGGGTCAATGGAACCCTTATCCGCTGGGTCGAAGGGATGTACCACCTGAAAATATCACAAAAAAGGCAGGTAGCCATATAC
AGACATTAATGGGATCCCAGAGCCTGCAGCATAGGTCCAGAGAGCAGCAGCACTATGAAGGGGGTATGAATAAAGTCACCATCCAGCAGTACCAGTCACC
ACTTCCTATTCAGATCCCTTCTGCTCAGCGAGGTCCTCAGTCAGGAAGGTGTTTGATCCAAACTAAAGGGCAACGGAGTATGGATGGATACCCTGAGCAG
TACTGTGTGAGAATAGAAAAGAACCCTGGGCTTGGATTTAGCATCAGTGGCGGGTTAAGTGGGCAAGGCAATCCTTTCAAGCCGTCTGACAAGGGTTCAG
CCTGATGGACCAGCGGCCGGTGTCCTGCAGCCTGGAGACAAGATTTTAAAGGCCAACGGACACAGTTTTGTACATATGGAGCATGAAAAAGCTGTTTCAC
TCCTGAAGAGTTTTCAGATCACAGTAGACCTAGTTATTCAACGTGAGCTTACTGTCTAAATATTTTTATAAATGTAAAAGATATGTTTAGACAGCTCTTA
TCCTTAAGGTAAATTTATACATAGGAAAAACATTTTGCCAATTGCTGGATCAATGGCAAAGATTAGTGCCAAATGTATAATATATGTTACTACTGACTTT
CAGATGTTCACAAAATACATATGAAAATCACATAGGTCTGTATTAGTTATAATTGTCAGCCAGAGGCTGTGCATTGGTGCAGTTTTTTAAATAAATACCT
TTCTTCCCAAAGTGGATTTCATATGGTTTCAGAGCATGGAAGCACACAGAAGCTATTAAATTATACTAATTGCCATGAAACACTGCCTCAAAATTGTATA
TGCCTTTTATATATTAAATAAATAAATCATAGTAGGATCTGCAAGTCATATTTTGACTTCTATAAAAAAAAATGATTACAAAACAGAATCATATTTTCTT
AACTAAGGATTTGATTGATAAGAAAGTATTTATTAAAGATGTAGATATATATACTGATTTAATCCCTTTTTATATAAAAAAAAAAAAAAA
5' EST: CX845499 [+]ggcatcccatgctcgcacctgaagggcatcttttaggcaccatgagctgctgccgccgctgccattgattcagttccttcccgtcccgcaccccggagac
cctaggaatagcgcccgcaaagcttcgctggagcctaatgggcaatttatcgtccctccaattgcagtgcctggagatgacaactaagcggaaaattatt
gggaggctggtcccatgcaggtgttttcggggtgaagaggaaattatatcggtgttggattattcccactgcagccttcagcaggtgccaaaggaggtgt
tcagtttggaaagaacattagaggagctttatcttgatgctaatcaaattgaagagttacctaagcaattgttcagttgccaggcccttcggaagctgag
tatacaagacaatgatctttcaaacctgccaaccactattgcaagtttagtgaatctcaaagagttggatatcagcaagaatggaattcaagaattccca
gaaaacatcaaatgctgcaaatgcttaaccatagttgaagccagcgtgaaccccatttcaaagctcccagatggttttactcagcttcttaacttgactc
agctgtaccttaatgatgcctttcttgagtatcttcctgctaactttggaaggctcagcaaattgcggatattggaattaagagaaaaccatttgaaaac
attaccaaagtctatgagtaaacttgcccagctggagaggcttgatctaggcaataatgagtttacagaactgcccagaggcctggaactaatacagaac
ttaaa
3' EST: CX845498 [+]aaaaagggattaaatcagtatatatatctacatctttaataaatactttcttatcaatcaaatccttagttaagaaaatatgattctgttttgtaatcat
ttttttttatagaagtcaaaatatgacttgcagatcctactatgatttatttatttaatatataaaaggcatatacaattttgaggcagtgtttcatggc
aattagtataatttaatagcttctgtgtgcttccatgctctgaaaccatatgaaatccactttgggaagaaaggtatttatttaaaaaactgcaccaatg
cacagcctctggctgacaattataactaatacagacctatgtgattttcatatgtattttgtgaacatctgaaagtcagtagtaacatatattatacatt
tggcactaatctttgccattgatccagcaattggcaaaatgtttttcctatgtataaatttaccttaaggataagagctgtctaaacatatcttttacat
ttataaaaatatttagacagtaagctcacgttgaataactaggtctactgtgatctgaaaactcttcaggagtgaaacagctttttcatgctccatatgt
acaaaactgtgtccgttggcctttaaaatcttgtctccaggctgcaggacaccggccgctggtccatcaggctgaacccttgtcagacggcttgaaagga
ttgccttgcccacttaacccgccactgatgctaaatcccagcccagggttcttttctattctcacacagtactgctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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