XB-CLONE-3297466
Clone Name: IMAGE:7662626
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Gene:
gpr78
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136127 [+]
CTCCCAACCACACCTGCTCCTTATTTTCTCCCTCACACTAACAGCCTCTCTCAGCCCGTCCCGGGAAGCCGCTGCTCTGAGTGCTGCTCTGAGTGCTGCT
CTGTATGCTGCTGTGTGTGCTTCTCTGAGTGCTGCTGTGCCACAGAATGCCGGGGGCAGCGGTGCTGTGAGAGGGAACACTATCAGCTCCCTGGGTTCCA
TGTGGCTCCTTGCTGGCATGCCTGCCACTCACTCTACTTCCAGAACCCTGGACAGCAACCTTATCCCATTGCTGGAGAGCTGCTCCTTGTGTGGCCAACC
CCTGGGGGTCCAACTCCGCTGACAGCATGTAGGGGGCCACTCTCAGCCTTCTGCCGCACTCTCTCTGCTCTGCACATGGGGCTCATGGATACACATTAAG
AAATGAACCCGGCAGAGGTTCTGCTGGCAATTTTCCTGATTGCGGTGCTCATCGTGTCCTTACTTGCCAACCTCCTGGTGGTGATATGCTTCTTGTACAG
CACCGAGATCCGCAAACAGGTCTCTGGGATATTCCTGGTAAATTTATCTCTCTGCAACTTGCTTCTGACTATCCTAAACATGCCTTCCACTTTCCTGGCT
ATCGTCAAGCACCAACAGCCCTTTGGCGAGAGCCTGTGCCAAGCCGTGGGCTTCCTGGAAACTTTTCTGACTTCTAACACCATGCTAAGCATGGCGGCTC
TCAGCATAGATAAGTGGATTGCTGTAGTGTTCCCTTTAAGTTACACCAGCAAGATGAGGTATAAAGATGCTGCGCTTATGATGGGCTATTCCTGGCTCCA
TTCGCTCACCTTTCCTTTGGTCTCTTACTTCTTCTCTTGGTTAGACTACAGTAGCATGTACGCTTCTTGCACTTTGCATGCCCAGGAAGAGGCTGACACG
CGGAGGTTTATGGTATTTACCATCGTGTTCCATGCTGCCACTTTCATGCTCTCTCTGCTTATTTTATGTTTTACATATTTAAAGGTGTTAAAGGTGGCGC
GGTTCCATTGCAGGAGGATAGATATAATTACCATGCAAACGCTTGTTTTGCTAGTTGACCTCCACCCCAGCGTGAAGCAGAGATGTCTCAGCGAGCAGAA
AAGGAGACGACAGCGGGCGACAAAGAAAATCAGTGTTTTTATAGGATCATTTGTGGTGTGCTTTGCGCCTTATGTTGTCACCAGGTTAATAGAACTTCTT
CCCTTTGTAAAAATAAACCGCTACTGGGGCATTGTAAGTAAGTGCCTTGCATACAGCAAAGCTGCGTCCGACCCTTTTGTATATTCCCTTCTGCGCCAAC
AGTACAAGAATGTTCTGCTGAACATTGTTAACAGAGTATTAAAAAGAGAACTTTACCCTTCGTCAGGCTATAACAGCTCCCTCGATACAGAGAATGACTA
CTGTTTGCACAGACCGAGCTAAGGCACTCCAGCGCTGCTGTGATATCAGTACCTTGATCCTTCATCAGAGGAACACATCATCATCATCTTCAGTTCTATT
ATGGAAAGTTGTCATAACCAAAGGTCACACTTTTGCTTTCTACCATTTTATGGTCCCTCGTTGCTTATATATACATATTTATATACTGTATATATATGCA
TTCCTCGTTTCTAGGTTCAAACTCAACCAAAACCTGAATCCAAAATGTTGCACTACAAAACTAAGGTGGGGTGGCTCGGCACTTTCTCAAAAAGTTCTAG
CCCAGTGTTAGCGCCCCCCAGAAGAACATTCTTTAGTGCTGATCTGAGCATACTATCCATGCCAACATAGAAGGGACCCAACAGGGAGGGCACAGTTGTC
TGGACCAGGCATGGAATCAGAGGGTTGCAGGATGTGTGGGCACTTACCATAGTGCCACTTTGTCATGGAGTAAATCTCTGGCAGGATAATGGATTCAGAT
TTCTAAATCTTCTACCTTATTCTCCAAGTTTGCTCTCCAGATCCGGACCCATTTTGTTTTTAAAGTAATGAATCAAAAATTAGTACTTAACCCTCTGGTG
TATGCTCAGAATTATCCATCTCATGTAGTTTGGTCCTAGTAGAAGCAAGCTAGAAATAACTGAAGCCAGTTTCACACAGTACTCCTTGTGGTACAACTGT
CCAACTATGCTCCACCATGAGCTAGTGGATAGGGACGGAATATGGCACTAAAATCTTTTCCCCAATGGCAGCAACATCCATGGTCTCTGCTGGTGTTTGT
TAAGCAGCAGAGACCATGGATGGTCTTATGTCTTATGCCAAAGCATAGACATGAACTGCCCATTCAGAACAAGTTGGTAGCTTATGAAGCAAAACTCATG
TCTGACCGATATGTGGCTAAAACTTGTCCATATATCAGATTGGAAAATCCCATTGGATAGGATGTTATTGACCAGTCAGTGCAGTCCTTGCTCAAATGGG
TCTGCCTAGGCACCTATTATGATCCTATTGTTGGCCTTCAGGTCAAGGGAAAAATTGTTCAAATGTCCCAGGTCTCCCTGCCAGCTTCTTATCTCTGTTC
TCCTTTGGATATAGTCATTAACAAGCCATAGAAAATAAAGTCAGCGCTCCTGCGAGTTAAAGATCAGATTAGCCCACTTTGCTCCACCACAACTGGATCT
TGTTTTTCCAACTTAATGTAGACATATTTCTAATTTAATATTATTTGGTAAAAAAATGTTTTCTCAGCCTTCATAATGAACAATATTAGCCATAGTAGAT
GTTGCAGTTGTGAGTCCACAGGGAGTGAGTCCACTATCTATGTCTTTTCAACAAGGCACTTTGATGAAATTATTACTACTTTGGTAAATAATATTCATTC
TCACCTTTAGAAAGCAACCTGATGGTTTTGGGACCATGGGCTCCATCTGAGATGATCAGGGGAATGTTGCAGCATGTTCACAGTTTGCCCCAGGCCTATT
ACCCATAGCAACCAATAAGAAGGCAGCATTTATGGATTTGTTGTTTTGAAGCATCTGTGCACAAACTATGACCCTCCGGCTATTGTTGAAACAACTACCA
GTTCCATAACAGCTGAAGGGCTGCATCACTAATGTACATAAATCTACAGTCATTACCCAGTATAATTTTATACTTTCATATATATTATATACATGATATA
ATTTTATACCATATACACAATTTTACACTTCTTGCTGCTCTAGCACTTGCATTTGGGGCACAACTAAATGACCCCTCTAATGTTCATGAATCAGGCCCAG
ATTTTTGGAATAGGCCACATAGGACAGACTCTAGGGCAATAGATTTTTTTCAGTGAGGCAGGCTGTCTCTAAAATGCAGAAAATAGCATTTGTGTCATAC
ATTTTGTTGCAGCAGAAAACTAATTATTTAGAAATTTTCCACGCATTTTCTTACCCAAAAAAATACATTTTTTTACACTGAAAAGAGCACCCACCCCAAT
GCATTTGACTAGGGGAACTCCCATTGACTCCATTGGTGTGGGAAAAACAAACGCAGAGAAAAAGATGCCCATAGACTCCAATGAATTTGCTGAAAATATT
GCCCATGGTGGGCCAATATATAGCCTACTTGTCTCACTGCCCAGTGATCTCTTGACTGGCTGCCTGACCAATGCCAAATGCGTGCTTAACATTACATTTG
AAGGCTGCAGTGTTTAATGGTCAGGGCCAGAACTGGCCAGTGGGACACTGGGAAAAAAACCCAGTGGGTCCTGCTGACCCAAAACTGATCCCTGGCAGGA
AGCATTGGTGCTAGGTAATTATAGTTATTCTAGGTAATTATGAGATATAGGGAGCCCTTGGGGAGTGTGGGGGGCCCCTTAGGTGGCAGCCCTGTTGAGC
CACGTACACCCCAGTCTGACCCTGTTGTTGGTTAAACTACCAGTTCCTGTGTATCAATAAATAAGTATTTGAGCTCATTAATACTGGCATTAACAATACT
ACTGGCCTATTATTAGAGACACGCAGACCACATCATGTAGATAAAAGGTAGCAGATTTAGTTACTTGGTACCCTACCTACATAATCCAACAGATTACCAA
CAGACACAGGGGCTTATTTATTACTGGTCGGCTGCAATGCAAGAACGCTAATATGTCCAGCGACAATATAAACATTACTTGACGAATGCAAAAAGAATTG
TATTTTTAGGTTCCGTTACTAAAACACTGAAGCCCAAGAAACACTCTCACAGCAATACATTCGCAGTATATACAGTACATTTCCCATCAATATGGCAGAT
GTTTAATGAGTGTTTATTGTTTTCTGTGCGAGGTTTAATATCTCAGGCTTTTTTTAAGTTTACATTTTTTTGTTTACATATATATATATAATTAGAGTCC
AAAGATTGTCCATTTTCAATAAACTACATATAAACTGAAAAAAAAAAAAAAA
5' EST: CX845509 [+]ctcccaaccacacctgctccttattttctccctcacactaacagcctctctcagcccgtcccgggaagccgctgctctgagtgctgctctgagtgctgct
ctgtatgctgctgtgtgtgcttctctgagtgctgctgtgccacagaatgccgggggcagcggtgctgtgagagggaacactatcagctccctgggttcca
tgtggctccttgctggcatgcctgccactcactctacttccagaaccctggacagcaaccttatcccattgctggagagctgctccttgtgtggccaacc
cctgggggtccaactccgctgacagcatgtagggggccactctcagccttctgccgcactctctctgctctgcacatggggctcatggatacacattaag
aaatgaacccggcagaggttctgctggcaattttcctgattgcggtgctcatcgtgtccttacttgccaacctcctggtggtgatatgcttcttgtacag
caccgagatccgcaaacaggtctctgggatattcctggtaaatttatctctctgcaacttgcttctgactatcctaaacatgccttccactttcctggct
atcgtcaagcaccaacagccctttggcgagagcctgtgccaagccgtgggcttcctggaaacttttctgacttctaacaccatgctaagcatggcggctc
tcagcatagataagtggattgctgtagtgttccctttaagttacaccagcaagatgaggtataaagatgctgcgcttatgatgggctattcctggctcca
ttcgctcacctttcctttggtctcttacttc
3' EST: CX845508 [+]tttttttttgtttttcagtttatatgtagtttattgaaaatggacaatctttggactctaattatatatatatatgtaaacaaaaaaatgtaaacttaaa
aaaagcctgagatattaaacctcgcacagaaaacaataaacactcattaaacatctgccatattgatgggaaatgtactgtatatactgcgaatgtattg
ctgtgagagtgtttcttgggcttcagtgttttagtaacggaacctaaaaatacaattctttttgcattcgtcaagtaatgtttatattgtcgctggacat
attagcgttcttgcattgcagccgaccagtaataaataagcccctgtgtctgttggtaatctgttggattatgtaggtagggtaccaagtaactaaatct
gctaccttttatctacatgatgtggtctgcgtgtctctaataataggccagtagtattgttaatgccagtattaatgagctcaaatacttatttattgat
acacaggaactggtagtttaaccaacaacagggtcagactggggtgtacgtggctcaacagggctgccacctaaggggccccccacactccccaagggct
ccctatatctcataattacctagaataactataattacctagcaccaatgcttcctgccagggatcagttttgggtcagcaggacccactgggttttttt
cccagtgtcccactggccagttctggccctgaccattaaacactgcagccttca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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