XB-CLONE-3297356
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Clone Name: IMAGE:7662790
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Gene:
slain1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121915 
[+]
GATGCAGCCCCTGTCCCTGCTGTATGGGAGCTATCAGAGATCCTGACTGCAGGTACCTGGATTTATCAGCCAGGGCAGCATCCTGGAACATTACCCCCTA
GGTGGAATTACAAATTAAGGCCGCTGGGGATTTGAGACACTGGCTATAGAACGCTGCAGCAGTGTGAGCCATGGCAGAGGTAAAGTGCAGCAGCAGCACT
GGGTCTCCCACTACTAATGGGCTGGTGGCGAATGCAGAGCTGGAGGTGAAGAAGCTGCAAGAGCTGGTGAGGAAGCTGGAGAAGCAGAATGAGCAGCTGA
GGAACCGAGCCAGTGCAGTGAGCAACTGTACGCCAAGCCCACACCTGCTGCTGCAGCATCCTCCTTCTGTGGTGCATCAGTCAGGGACATGCATCCTCTC
TTCCCCTGTGCTCACACGGCCCGCAGGGCTCTGCCTTCCCAGTCCCGTGCCCACCTTGCTGTGCACTTCAGCAGTGGCCGGCAGCCTGTTTTCCCCAGAA
TCCATGGGCTGTTTTAGTACCAACAAAAGGCTGCATTTGTCCTGCATCAATGCTGCAGAGGGGCCCCAAAATGACTGCCCAGTTCCAGGATCTACCCTAC
TGGATGAGGTGCAGGTTCTGGATTTGGAAGACGGGTATTGCAGTGGCGATGAGGATACCTGGTTATATGTCTCACCTACAAAGGATCAGAGGACATTTGA
CTCATCCGTGAGTCCACTGCAGTGGTGTAGGCAAGTTCTGGACCATCCATCGCCTGAGATAGAAGCAGCTAAGCGATCCCTGTGCTTCAGACTAGAGCAA
GCTGGTCGATGGAGGAGTCTCTTTTCCACTCCTGTATCCATGTCTTTTCCTTATAGTCCTGTTGCGAGACTCACTCCATATTGCAACACTCCCAGCGTCC
TTAAAAACACAACTAAAGCAATACTAACACCTGAGAAAACAGGTTACACTGTAAAGACTTCCCATCTGAGCCCCCAGTCTTCGGTAGACAGTGAACTGAG
TACGTCTGAGCTAGAAGATGATTCCATCTCTATGGGATACAAACTACAAGATCTCACAGATGTTCAGATCATGGCTAGATTACAGGAAGAAAGCCTTAGG
CAAGACTTTGCAACTACCTCTACCTGCAGTTCAGTATCACGGCCTCGCTCCAGCTTTTCACTGTATTCTGGGAAGAAACTATCATGCTCTAGTGATCAGG
ATTCAGATCGCTATAGTGTTGAAGAAGATGATGAAGATTTTGATCATTTGCCACCACCTCAGCCTCGACTGAGCCGTTGCTCTCCACTCCAGAGAGGCCT
TTCTCATTCTCAGACTTTTTCTAGCATTAGAGATTGCAGGAAGAGTCCAAGCTCCCAATTTTTAAATGGATATCAGCAATATAACTATTCCTCTCAGCCT
CAAACAACAGAGCAGTCACAAAGCAGGACAAATGCAGATAAACTCCGCAGAAGTATGCCTAATTTGGCTCGAATGCCAAGTACAAATAATAACCCTGTTG
TGTCAATGACCACTGTGCGCAACAGCCAGAGCTTTGATTCCAATCTGCATGGTGCAGCAAATGGAGTTTCCAGAATGCAGTCCAGTATTCCTTCTCCAGG
CCAGCTTCAGCAAAGAGTACACAGTGTAGGCCATTTTCCAGTGTCTGTAAGACCCCCTCTGAAAGCCACTGCCTACGTCAGCCCGACTGTGCAAGGTGGA
AGTGGTATTCCTTCATCAACTAGTTTACAGTCATTATCTAGCTCTGGAATTCCTATGCCAAACAAGACCACAAGTGCAGCAACTATTGGACGCAGTGCTC
TTCCAAGGCCAGCATTATCAACTGCAAATGGCAGCAGCATACCCCGCAGCAAAATAGCACAGCCACAGAGAAGTTTTCTTCAACCACCAAAAACCTTGGC
TTCACTGAGCACGCTGCGAGATGGAAATTGGAGGGATGGATGCTACTGACATCTTTTAAAGGAGAGCACCTGTGTATAAAGAAAGCAATGAAGAGTTCTA
CATGTCCCAGCTGGCTGGGAGAAACATGTATATAACTCTTTGAGATTTTATTATTTGTAATATATGTTTTATGTCAACAAATGATCATACTGTAGCTCTT
ACATGTTTGACGTGAGATCATTTCAGATCAGCTAACTGAAGTTCAGTCCCTCACATGTGCAAGATTCTTGAGGAGAAACTGTTGAGGCTCCCTCTGCACG
TATGGGTTCATACATCATGGTAGAACATACAACATATGCAAAGAATTATCACTTTGTTCTGATTTACACATAGTAAATCACATTTTCAACTAACTGAACT
TTTTTTAATGATAACCAACACATTCATGCTAAAAGCGGGGGTTTTTTACACTGAAGTTCTTGGAGCACTGCAAACTATAATATGCCACACTTTTTTCATT
TTTGCTTTTGTTTTATTAAAGGCAAGTACATACAGACGCAAAATTTGTGACACTGAACAAAGCTCAAAATGCTGCTTAATGATTTGTGCCATAGATGCCA
ATTTATTTTTGGCATTATTATCATTTGGTGTGTCTTGGGAACCAACTGGTAAAATATCCATGGAATATGTGTGTTTTTTCCTTTTCTCTCTCTTTCCTTT
TTGTTTGCAAAAGCACAAGGGTATAAAGTCACTACAGTTTGCTATGGAGCCATCTTAAAACCGTCCTCGTTTTTGTGCATTTACATGTTTATTTATTTAA
CTATTTAATGATTTGCAATCATTGAATATTGATTGACTTTTTGCAGCTGTACTGTATTATCTTCTAACAACAAGGGGATTTGCTTTTTTATTATTAAAAC
TGGTTTACTTGCTGTCAAATTCTGGAACATAAGCTCTTAAATGGACAGAAAACATTTTGATAGGCATTTTACAAAATTTTTATTGCACTAATGATTTAAT
ATGACTGTATGATTTCATAATTATGAAAACTGCATACTACTGTTTTGGAATGTTTCAGTAATAAAGAATATTTTCATCAAAAAAAAAAAAAAAA
5' EST: CX845668 [+]tgcagcccctgtccctgctgtatgggagctatcagagatcctgactgcaggtacctggatttatcagccagggcagcatcctggaacattaccccctagg
tggaattacaaattaaggccgctggggatttgagacactggctatagaacgctgcagcagtgtgagccatggcagaggtaaagtgcagcagcagcactgg
gtctcccactactaatgggctggtggcgaatgcagagctggaggtgaagaagctgcaagagctggtgaggaagctggagaagcagaatgagcagctgagg
aaccgagccagtgcagtgagcaactgtacgccaagcccacacctgctgctgcagcatcctccttctgtggtgcatcagtcagggacatgcatcctctctt
cccctgtgctcacacggcccgcagggctctgccttcccagtcccgtgcccaccttgctgtgcacttcagcagtggccggcagcctgttttccccagaatc
catgggctgttttagtaccaacaaaaggctgcatttgtcctgcatcaatgctgcagaggggccccaaaatgactgcccagttccaggatctaccctactg
gatgaggtgcaggttctggatttggaagacgggtattgcagtggcgatgaggatacctggttatatgtctcacctacaaaggatcagaggacatttgact
catccgtgagtccactgcagtggtgtaggcaagttctggaccatccatcgcctgagatagaagcagctaagcgatccctgtgcttcagactagagcaagc
tggtcgatggaggagtctcttttc
3' EST: CX845667 [+]gaaaatattctttattactgaaacattccaaaacagtagtatgcagttttcataattatgaaatcatacagtcatattaaatcattagtgcaataaaaat
tttgtaaaatgcctatcaaaatgttttctgtccatttaagagcttatgttccagaatttgacagcaagtaaaccagttttaataataaaaaagcaaatcc
ccttgttgttagaagataatacagtacagctgcaaaaagtcaatcaatattcaatgattgcaaatcattaaatagttaaataaataaacatgtaaatgca
caaaaacgaggacggttttaagatggctccatagcaaactgtagtgactttatacccttgtgcttttgcaaacaaaaaggaaagagagagaaaaggaaaa
aacacacatattccatggatattttaccagttggttcccaagacacaccaaatgataataatgccaaaaataaattggcatctatggcacaaatcattaa
gcagcattttgagctttgttcagtgtcacaaattttgcgtctgtatgtacttgcctttaataaaacaaaagcaaaaatgaaaaaagtgtggcatattata
gtttgcagtgctccaagaacttcagtgtaaaaaacccccgcttttagcatgaatgtgttggttatcattaaaaaaagttcagttagttgaaaatgtgatt
tactatgtgtaaatcagaacaaagtgataattctttgcatatgttgtatgttctaccatgatgtatgaacccatacgtgcagagggagcctcaacagttt
ctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.| Anatomy: brain | Stage: frog |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI |  |
Usage:
Suppliers:
