XB-CLONE-3297071
Clone Name: IMAGE:7663326
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Gene:
syt7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121916 [+]
GAGAGAAGGACACAGGACCCCCCACAAGTGGCAGCTCAGCCCGTCCCCGCCCTCGACCTCAACCCACCTTGCAGGCTTTTGTGTGTGTTTTTGTATTATT
GTGAAGTACAAAGGGAAGGGACAAGAGTAGAGGGATCAGCGGAGGAGAAGAGGCAGTCAGGAAGATGCACATTAACCAAGAGGCTTCCAACACCAAAGGA
GCTCCCACCCGAGACGTTATCCTGGTCTCTACCGTCCTCACTTTGAGCCTCAGCATCACCATCATCATGTGTGGGCTCTGCCAGTGGTGCCAGCGTAAAC
TGGGAAAGCGCTACAAAAACTCTCTGGAAACAGCGGGGACTCCAGACTCCACTAGAGGAATCCGCAGCGAAAAGAAAACTTTAAATGGCACTGTATTGTC
CAGTAGTAAGTCAGCAGCTTGTGGCACATCTGGGTTACTGGACAAAGATGGCCGGCTCGGGGACAAACAGTTTCGCAGTGACAGCATGAAGATCAGCATG
TCCTCGCAAAGCCACCACAGCGAGCCAGTCCTGGGGAAGTCTGGGAGAGGTAGATGGCACACGGTTCAGAGCCACCTTGCATCTGGGAATCTCAGTCGAA
CCAATTTTGAGGACCCCACCCTGACAGCAGCCACTACCCTCGACCATATCCCCATCTCTGCAGGAGACATGAAATGCCAGCGTCCCCGCACGCTCCTCCG
CCAGCAGAGTCTCCAGCAGCCTCTCAGCCTCCACCAGAAGCACAATCACTGCCAGCCAACAACCAGTCAAAGCCTGGGGCAGCTACAGCTGCAGCATCAA
CCCCCTTCCACCAGCAGCAGCAACACCAGCAACACTCGTCCCCAACGCACGGGTCAGAACCGACCCCCAACCTCAGCTGGATCCAAGCACCGGCCAGCTG
GCATCAGGAACCGAACCAACGCTGGTAGCTGGGACTATGTGGTTGGTCAAATTCGAAACCGGGGCCTGGACATGAAATCTTTCCTGGAAGGCCGCATGGT
GGTGCTCTCCTTGGTGCTGGGTCTCTCCGAGCAGGACGACTTTGCAAATATCCCTGACCTACAGACCAATGGCTCGCAACAGAACCAGAACACTCAGGGC
GACAAGAGATTACAGACAGGTGGTAAAACCACAAGTGCGTCAGGAGTGCCGGGGCAAGGGCAGTCAGAAGATCCTGATCGCAAAACAGATCAACATGCTT
CTGTGTCCGATCTGGTGAACTCTCTGACGAGTGAGATGCTCATGCTGTCTCCTGGGTCGGACGAAGACGATGGTCACGACACTTCAGGAAGGGAGAACCT
GGGTCGCATTCAGTTCAGTGTGGGGTATAATTTCCAGGAGTCTACCCTCACGGTTAAGATCCTGAAAGCCCAGGAGCTGCCTGCCAAGGACTTCAGTGGA
ACCAGCGACCCCTTTGTGAAGATCTATCTGCTGCCAGACAAGAAGCACAAGCTAGAGACCAAGGTCAAGAGGAAAAACCTCAATCCACACTGGAATGAAA
CCTTCCTCTTTGAGGGTTTCCCTTATGAAAAAGTGGTGCAGCGGGTTCTGTATCTCCAGGTATTAGACTACGACCGATTCAGCCGCAACGACCCCATCGG
AGAGGTCTCCATCCCCCTAAATAAGACAGACCTGACACAGATGCAGACCTTTTGGAAGGAGCTGAAACCGTGCAGCGACGGAAGCGGGAGTCGAGGGGAA
TTGCTCCTGTCTCTGTGCTACAACCCTTCCACCAACGCCATAATTGTCAATATCATAAAGGCACGCAACCTGAAAGCCATGGATATCGGCGGCACCTCAG
ACCCCTATGTGAAAGTGTGGCTCATGTACAAGGATAAAAGAGTTGAGAAGAAAAAGACTGTGGTTATGAAGCGCTGCCTGAACCCTATTTTTAATGAGTC
GTTTATCTTCGACATCCCTACTGAAAAGCTCCGGGAAACAACCATTATCATCACTGTCATGGACAAAGACAAGCTGAGCAGGAACGACGTCATTGGCAAG
ATCTACCTGTCTTGGAAGAGTGGCCCCGGGGAAGTGAAACACTGGAAGGATATGATCTCTCACCCCAGAACGGCTGTAGCGCAATGGCATCAACTCAAGG
CCTGATTTGGTGGCCTCTTTGCACTCTGTCATCTCCAGAAGTCCTCGGTTACATTCAACCAATGCATTGGCCACACTCTTACTGTCTGCACAACCACAAC
CTGCAGGCTTCTCCAAGGAACCGCCAGCTTGGATCTCTGGGTAACCGAAAGCCCCAGGGCCAGACTGTGCCACCTGTACTTGTTATACTAGGCACAGCTG
CGGTTCCCCTCCTGGCCTCACAAGGCGTGGCGCACACCATAAAGCAGTGTTACAGAGACGCTGAAAAAGCTTGGGCGAGATGGCCCTGTAGAAAGCCAGT
CAGTATGCTGCGGCACCTGCAAACTTTCCATGGCATCTTCAAACACGTGGCAGCCACAGGAGCGCATCTAGCGTGCGTCAGTAGTGGTGGCCGGCGCTGC
AGCTCGCCTTTTGTAGTTGTGCAAAGGTGCACTAAACCCTCTTATTCTATTTTTTGGGGTTTTTAATCTTTGTTTAAATTTAAAAAAAAAAAAAGCAGTG
ATAATGAGCAATACCGGAGAAATTGCTACAGAGAAACATCGCTGGAACATGGAGGCAGATTACAAAGCATTCCGGTCCTCAGACTCTTTGCCATTTGTGC
GTGGTGTTCCGTGTCCAACGTGTGTAAAATAATGTGTGTGCGTGGGTGTTTTATTCTCCCACAATTCACCCTTTTTATCCTCTCTCCTCCCTTTGGCGGA
GTCGGGGGTAGTGCTCTGGTACACTTGCATATCTTCATATCATAATAATTTGTGTAATTTGTAGCTTCCTTTGTTGTCCTTTATTTTTTTAATTCTGTTT
TTTTTTTCACAAAATGGTTTCCTTTTTTCACAGTTTCACTAAAAAAAGGAGAAAAAGTTAAAAAAAAAAAAAAA
5' EST: CX846093 [+]agaaggacacaggaccccccacaagtggcagctcagcccgtccccgccctcgacctcaacccaccttgcaggcttttgtgtgtgtttttgtattattgtg
aagtacaaagggaagggacaagagtagagggatcagcggaggagaagaggcagtcaggaagatgcacattaaccaagaggcttccaacaccaaaggagct
cccacccgagacgttatcctggtctctaccgtcctcactttgagcctcagcatcaccatcatcatgtgtgggctctgccagtggtgccagcgtaaactgg
gaaagcgctacaaaaactctctggaaacagcggggactccagactccactagaggaatccgcagcgaaaagaaaactttaaatggcactgtattgtccag
tagtaagtcagcagcttgtggcacatctgggttactggacaaagatggccggctcggggacaaacagtttcgcagtgacagcatgaagatcagcatgtcc
tcgcaaagccaccacagcgagccagtcctggggaagtctgggagaggtagatggcacacggttcagagccaccttgcatctgggaatctcagtcgaacca
attttgaggaccccaccctgacagcagccactaccctcgaccatatccccatctctgcaggagacatgaaatgccagcgtccccgcacgctcctccgcca
gcagagtctctagcagcctctcagcctccaccagaagcacaatcactgccagctcacaatcagtcaaagcctggggcagctacagctgcagc
3' EST: CX846092 [+]tttttttttttttttaactttttctcctttttttagtgaaactgtgaaaaaaggaaaccattttgtgaaaaaaaaaacagaattaaaaaaataaaggaca
acaaaggaagctacaaattacacaaattattatgatatgaagatatgcaagtgtaccagagcactacccccgactccgccaaagggaggagagaggataa
aaagggtgaattgtgggagaataaaacacccacgcacacacattattttacacacgttggacacggaacaccacgcacaaatggcaaagagtctgaggac
cggaatgctttgtaatctgcctccatgttccagcgatgtttctctgtagcaatttctccggtattgctcattatcactgctttttttttttttaaattta
aacaaagattaaaaaccccaaaaaatagaataagagggtttagtgcacctttgcacaactacaaaaggcgagctgcagcgccggccaccactactgacgc
acgctagatgcgctcctgtggctgccacgtgtttgaagatgccatggaaagtttgcaggtgccgcagcatactgactggctttctacagggccatctcgc
ccaagctttttcagcgtctctgtaacactgctttatggtgtgcgccacgccttgtgaggccaggaggggaaccgcagctgtgcctagtataacaagtaca
ggtggcacagtctggccctggggctttcggttacc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: