XB-CLONE-3292380
Clone Name: IMAGE:7665757
|
Gene:
entrep3
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX851392 [+]
tcctcccctaactccactgctgcctcctgctcccagggctcgcgcctcgaagtgccacctcgggcaacttggagtgcatttcaccccaggacaccctctt
gcagagtatgatagagtgcgaggagtttgtcccaccggtgcccccacctccctattaccccccagaatacacctgcagctctgagacggatgcacagagc
atcacttataatggctcaatggacagcccaatccctctctacccaactgacttccctccctcctatgagagcgtcatgggaatccgcgaggacagccagg
caacgttgtttgatgcccaggtcaacgaggtctcgtgcctgagtgtgtccgacagaatcggttccaccggtcacagcggcaatgaagacagcagtccctc
tgaagactcggttctcctggagatgcagggttccatccgttctgtggactatgttctgtaccggtccatccaacgcagcagggcagattactgtctcagt
gtggactgtggccagtgtgggcaccatcagcagagcccgactctcagccaccagggcccatttgaggagatcccattgcctcatgccaaggggggcagat
ccttctcatgctccacgccgagtgctggtgatgatgttctgccgaatgtggttggtgccgtggtgactcgcagctgtaaccgcctggagaccattggccg
atctgttggtccttgtttcccggaggtgcgactganagcaaaggtttctgggaggtctgtacaaagcagggggtccggttgctccctccatcacagctcc
ccctgccagacacccgactgcccccaccgcaagtacagcgagaacac
3' EST: CX851391 [+]gcaataaatattaataaacatacttaacagtagcattgcgcaagagctaacaatccgggggccgcaccccgagggccacatgatgttgcagtttctcagt
ccgtggttaacccttttctgcccacaaggcgggactgacagcaaagcaataaatgaacatgcaaagtaaaggggggcattcccaaaggctcctgtatgaa
tgtgagggcaaggtgcccacggtgggtcacatactggtactgccaccctaaggtgggtcacatactggtactgccaccctgttctgggagaaccagcagc
ctggactggcactgacactggggggcactactgccattattaattctgcccaccctaggaacagtgccctgcaactgaccaacatgcccaaactggggag
agtggggcattgtgggtaaaacagggtggtttgctgccaagttgggcactttgcactcgttggtacatgagccattgatccctcacatgagttcccccag
tccatctgtctgtgacgccccccggggggcagttccatgtagggtaccgactgggtataactgtgtgccagggaagtggcatcagaatatggtggctgcc
aacatcacagcccaggcgttggccacactgccagccttgtatc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: