XB-CLONE-3291444
Clone Name: IMAGE:7666630
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Gene:
syt3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121381 [+]
GCCAATACAGGAGGAAGGGAGAGCTCTCACTCACACACACACTGAGCAATGGGATCCTGCGTGGGGATTGTTGTGACCCAGAAATTGCTCCACATCTGCA
AGTAAAGGGCTGAGAGCGGGCGGCGGGGCGGAGGGACGGGCGGCCGGAGCAGCAGTGACTCGGCGAATTGCAGCGCACGGATAACAGGAAGGGGATGAAT
AATGATTTGATTTCTCCGCCGAGCTCCGGTCTGACTGCCGCCCGGCTGGGAGGATGCAAAGAGCGGACAGGACTGCCGGATAAGGGAGAAGCCGTAAATG
CAAATGTTGGCACCTGCAAGCAAAACTTTCCACACAAGACAGTTACAAAGTTGCTGGGCAAGTTTTTCAGTGGAAAGTAGCTGGAATGGCGGGAAGAAGA
AAGCATGAGAATGGTGTGACCCAACTGGAAAGGTACTGAAGAACCCCTCCCTGGCAATCGAGACACAGCCCAAACCACTGGAGAGGGACCTTCCCAGTAC
TGGAGTAGGAGGGTTGGCCAGTTTCAAGTGATCATTTGAATGGAGAGTGAAGACAGGTGCTTCCCCAGCTTTCTCTATAAAAGTGCTGAGAGATGGTAGT
GCGAGATGTCCGGGGACTATGAAGAGGATCTTTGCCGCAGCACTATGATGCTGATGAGGGAGCTATGCTCTAGCATGCAAAGCTACGCTGAGAATGAAAA
ATGTCTGGAATTTTACGACTTGTTGCGAAGCCAAGGGATGACACACATCAGAAACACAGATGTGTCCGTCAGCCTGCTGTCAGTTATTGTCACCTTCTGC
GGGATTGTTTTATTAGGAGTCTCACTCTTTGTTTCCTGGAAGTTGTGTTGGATTCCATGGAGGGACAAAGGTTCTTCACAGACACAACGCAAAGATCATG
GACATCATGGCCATCTCCATCACTCTCACTATGGGGACTTGCTAGCAGACCGTGTGGAGCTTGGACCTGAGATGCCTGAACGTTCCTATTTAGATATGGA
CTCCTACCCAGAAACCAACATTAAAATTAGTCAGACATCTCCTGACATACCTGTGGATAGCCAACCGCCCAAGGAATCTCAGATTCCATATGCTCAGTCT
CACCAGCAAGTGGCAAATATAGCCCCGGCAAATGTAGCCCCGGCAAATGTAGCCCCGGCAAATGTAGCCCCAGCCAACAGGTATAGTACTCTGCCCAGAC
CCATGACCCAACAGTTGTCTAGTCCTGATTTTTCAGGTCATGTGGATGAAAAGCCAGAACAAGCCACCAGCATTGGACAGATAAAGCCGGAGTTGTACAA
GCAGCGCTCGGCCGATGCAGAGATGAAGAAGCAGGAGGCCACCAGCTGTGGCCGTATCAGTTTCATTCTTCGCTATGCCTACAACTCTGAGCAGCTAGTG
GTAAAGATCCTGAAGGCATTAGAACTACCAGCAAAGGATGCAAATGGTTTCTCAGACCCCTATGTAAAGATGTATCTGCTGCCTGATCGCAAGAAAAAGT
TCCAGACCAAGGTTCACCGCAAGACACTTAACCCAATCTTTAATGAGACATTCCATTTCAATGTGCCCTTTAATGAACTGCAGAATCGCAAATTGCACTT
CAGTATCTATGACTTTGATCGCTTCTCCCGTCACGACCTTATCGGACAAGTGGTGCTTGATAACCTGCTAGAGTTTTCAAATGCTACAGATGAGACTCCC
ATTTGGAGAGACATCTTGGAGGCCAGTTCTGAGAAAGCAGATCTGGGTGAGATAAACTTCTCTCTGTGTTACCTCCCAACTGCCGGTCGCCTCACTGCTA
CCATCATTAAAGCAACGAACCTGAAAGCTATGGATCTGACAGGATTCTCCGATCCTTACGTAAAAGCCTCTCTCATCTGCGAGGGAAGGAGACTGAAGAA
AAGGAAAACATCAAAAAAAAAAAAAAA
5' EST: CX853098 [+]ccaatacaggaggaagggagagctctcactcacacacacactgagcaatgggatcctgcgtggggattgttgtgacccagaaattgctccacatctgcaa
gtaaagggctgagagcgggcggcggggcggagggacgggcggccggagcagcagtgactcggcgaattgcagcgcacggataacaggaaggggatgaata
atgatttgatttctccgccgagctccggtctgactgccgcccggctgggaggatgcaaagagcggacaggactgccggataagggagaagccgtaaatgc
aaatgttggcacctgcaagcaaaactttccacacaagacagttacaaagttgctgggcaagtttttcagtggaaagtagctggaatggcgggaagaagaa
agcatgagaatggtgtgacccaactggaaaggtactgaagaacccctccctggcaatcgagacacagcccaaaccactggagagggaccttcccagtact
ggagtaggagggttggccagtttcaagtgatcatttgaatggagagtgaagacaggtgcttccccagctttctctataaaagtgctgagagatggtagtg
cgagatgtccggggactatgaagaggatctttgccgcagcactatgatgctgatgagggagctatgctctagcatgcaaagctacgctgagaatgaaaaa
tgtctggaattttacgacttgttgcgaagccaagggatgacacacatcagaaacacagatgtgtccgtcagcctgctgtcagttattgtcaccttctgcg
ggnatgtttattaggagtctcactc
3' EST: CX853097 [+]tttttttttttttttgatgttttccttttcttcagtctccttccctcgcagatgagagaggcttttacgtaaggatcggagaatcctgtcagatccatag
ctttcaggttcgttgctttaatgatggtagcagtgaggcgaccggcagttgggaggtaacacagagagaagtttatctcacccagatctgctttctcaga
actggcctccaagatgtctctccaaatgggagtctcatctgtagcatttgaaaactctagcaggttatcaagcaccacttgtccgataaggtcgtgacgg
gagaagcgatcaaagtcatagatactgaagtgcaatttgcgattctgcagttcattaaagggcacattgaaatggaatgtctcattaaagattgggttaa
gtgtcttgcggtgaaccttggtctggaactttttcttgcgatcaggcagcagatacatctttacataggggtctgagaaaccatttgcatcctttgctgg
tagttctaatgccttcaggatctttaccactagctgctcagagttgtaggcatagcgaagaatgaaactgatacggccacagctggtggcctcctgcttc
ttcatctctgcatcggccgagcgctgcttgtacaactccggctttatctgtccaatgctggtggcttgttctggcttttcatccacatgacctgaaaaat
caggactagacaactgttgggtcatgggtctgggcagagtactatacctgttggctggngctacatttgccggggctacatttgccggggctacatttgc
cggggctatatttgccacttgctggtgagactgagcatatgggatctg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: