XB-CLONE-3289744
Clone Name: IMAGE:7668641
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Gene:
chrnb2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136136 [+]
ATCGGGTCGGTTCAGCACCACGGACAGCGGCCGGTAGGAATGAGGCGATGAGATGATCCGGACCGGGATGGCCCCCCTCCTGGCGGCCCTGTACCTCCTG
CTGGGGCTGCTGCCAGGCTGCCTGGGGACGGACACAGAGGAGCGCCTGGTGGAACATCTTCTGGACCCTTCTCGCTACAACAAACTTATCCGCCCGGCAA
CAAATGGCTCAGAGCAAGTCACCGTTCAACTAATGGTCTCATTGGCCCAACTCATCAGCGTGCATGAGCGGGAGCAGATTATGACAACTAATGTCTGGCT
GACTCAGGAGTGGGAGGATTACAGACTCACCTGGGACCCAGCGGAGTTTGATGACATGAAGAAAGTCCGACTCCCATCCAAGCATATTTGGCTCCCCGAT
GTCGTGCTGTACAACAATGCCGACGGGATGTACGAGGTGTCGTTCTACTCCAACGCGGTGGTGTCCCACGACGGCAGCATATTCTGGTTGCCCCCTGCCA
TATACAAGAGCGCCTGCAAGATTGAAGTCAAGCACTTCCCCTTTGACCAGCAGAACTGCACCATGAAGTTCCGCTCTTGGACGTACGACCGCACAGAGCT
GGACCTGGTGCTGAGGAGCGACGTGGCCAGCCTCGACGACTTCACGCCCAGCGGCGAGTGGGACATCATCGCCCTTCCCGGACGGAGGAACGAGAATCCC
AACGACTCCACCTACGTGGACATCACCTACGACTTCATTATCCGGCGCAAACCCCTCTTCTACACCATCAACCTCATCATTCCCTGCATCCTCATCACCT
CATTGGCCATCCTAGTCTTCTACTTGCCCTCGGACTGTGGGGAGAAAATGACTCTGTGTATCTCGGTGCTTCTGGCTCTCACTGTATTCCTGCTCCTTAT
ATCCAAGATCGTACCCCCCACTTCTCTGGATGTGCCACTAGTGGGCAAGTACCTGATGTTTACTATGGTCCTGGTGACATTTTCCATCGTGACCAGTGTC
TGCGTATTGAACGTGCACCACCGCTCCCCAACCACACACACTATGCCACCCTGGGTGAAGGTAGTCTTCCTGGATAAGCTGCCCACCTTACTCTTTATGA
AGCAGCCCAGGCAGAACTGTGCCCGCCAACGGTTACGCCAGCAGAGGCAAAGCCAGGAGAGGGCTGCCGGTAGCTTCTTCCTCCGGGACGGCGCCAGGTC
CTGCACCTGTTATGTTAACCAAGCCTCGGTGAAAAAGTACGGAGCCCAGCTTTCCGAGCTCCCCGAAGGAGTCAACGGCTTCCGGGACAGACAGGGCAAG
GTGCGCCAGTGTCTGTGCGGCCTGCAGGAAGCGGTGGACGGAGTGCGTTTCATCGCCGACCACATGAAGAGCGAAGATGATGATCAGAGCGTGAGCGAGG
ACTGGAAATACGTGGCCATGGTTATCGACCGCCTCTTCCTGTGGATCTTCGTGTTCGTCTGCGTTTTCGGGACGATCGGCATGTTCCTGCAGCCGCTGTT
TCAGAACTACACGACCAATGCGTTGGTACATATGAACCACGCGGCCAACTGAGAAACATTGAGGGCCCTCAGACCTGGTCGCTGGCCCAAATCTACCTTA
GAGATTATTCCCACCCACCCCCGTCCCCAACACAGTGAAGAAATCCTGGTGGAACCTGGGAATAGCTCACCCCTCCCTTCACCACTGAATTAGTACAAAT
TTGGGGAACTTATTCCTCTGCACACGTTTATTGACCCAGCAAGTTCCAGAATCCAGGTGAGAGGGGTCCATGCGAATTGAACCCCCCCTACAGACTCTTA
TATTAATCTCAAGCAATAAAGATGAGCTTCTGGATCTGCAAAAAAAAAAAAAAA
5' EST: CX856050 [+]atcgggtcggttcagcaccacggacagcggccggtaggaatgaggcgatgagatgatccggaccgggatggcccccctcctggcggccctgtacctcctg
ctggggctgctgccaggctgcctggggacggacacagaggagcgcctggtggaacatcttctggacccttctcgctacaacaaacttatccgcccggcaa
caaatggctcagagcaagtcaccgttcaactaatggtctcattggcccaactcatcagcgtgcatgagcgggagcagattatgacaactaatgtctggct
gactcaggagtgggaggattacagactcacctgggacccagcggagtttgatgacatgaagaaagtccgactcccatccaagcatatttggctccccgat
gtcgtgctgtacaacaatgccgacgggatgtacgaggtgtcgttctactccaacgcggtggtgtcccacgacggcagcatattctggttgccccctgcca
tatacaagagcgcctgcaagattgaagtcaagcacttcccctttgaccagcagaactgcaccatgaagttccgctcttggacgtacgaccgcacagagct
ggacctggtgctgaggagcgacgtggccagcctcgacgacttcacgcccagcggcgagtgggacatcatcgcccttcccggacggaggaacgagaatccc
aacgactccacctacgtggacatcacctacgacttcattatccgggcgcaacccctcttctacaccatcaacctcatcattccctgcatcctcatcacct
cattggccatc
3' EST: CX856049 [+]tttttttttttttttgcagatccagaagctcatctttattgcttgagattaatataagagtctgtagggggggttcaattcgcatggacccctctcacct
ggattctggaacttgctgggtcaataaacgtgtgcagaggaataagttccccaaatttgtactaattcagtggtgaagggaggggtgagctattcccagg
ttccaccaggatttcttcactgtgttggggacgggggtgggtgggaataatctctaaggtagatttgggccagcgaccaggtctgagggccctcaatgtt
tctcagttggccgcgtggttcatatgtaccaacgcattggtcgtgtagttctgaaacagcggctgcaggaacatgccgatcgtcccgaaaacgcagacga
acacgaagatccacaggaagaggcggtcgataaccatggccacgtatttccagtcctcgctcacgctctgatcatcatcttcgctcttcatgtggtcggc
gatgaaacgcactccgtccaccgcttcctgcaggccgcacagacactggcgcaccttgccctgtctgtcccggaagccgttgactccttcggggagctcg
gaaagctgggctccgtactttttcaccgaggcttggttaacataacaggtgcaggacctggcgccgtcccggaggaagaagctaccggcagccctctcct
ggctttgcctctgctggcgtaaccgttggcgggcacagttctgcctgggctgcttcataaagagtaaggtgggcagcttatccaggaagactaccttcac
cccaggtggcatantgtgtgntgttggggagcggtggtgcacgttcaata
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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