XB-CLONE-3289014
Clone Name: IMAGE:7669497
|
Gene:
atg4b
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC121870 [+]
GTGAAAGGAATGATGGACGCAGCTACACTCACTTATGATACCCTGCGCTTTGCCGATACTCCCGACTTTCCTGAAACAGCGGAGCCAGTTTGGGTGCTGG
GAAGGAAATACAGTGCATTGACAGAGAAAGAGCAGCTCTTGAATGACATCACTTCCAGGCTCTGGTTTACCTACAGAAGGAACTTTCCAGCAATAGGGGG
CACAGGGCCCACGTCTGACACGGGCTGGGGCTGTATGCTACGATGTGGCCAGATGATCTTTGCTCAGGCTTTGCTCTGTAGACACATAGGAAGAGACTGG
AGATGGGACAAACAGAAATCCCAAGGAGAATATCTGAACATCCTTACAGCTTTTCTGGACAAAAAAGATAGTTATTATTCCATCCACCAAATTGCACAAA
TGGGAGTTGGAGAAGGCAAATGCATTGGCCAGTGGTATGGTCCAAACACAGTGGCGCAGGTCCTGAGGAAACTTGCGGTTTTTGACCAGTGGAGCTCTAT
TGCTGTTCATATAGCTATGGATAACACAGTTGTGATGGATGAGATACGCAGATTATGCAGAGCTGGAACCAATGAGAGCTCCGAGGCTGGGGCCCTTTGT
AATGGCTATACAGGCGTCTCTGACCCCAGCTGCTCCCTATGGAAGCCTCTTGTGCTACTCATACCTCTTCGGCTGGGACTAAGTGATATCAATGAAGCCT
ATATTGAAACACTAAAGCACTGCTTTATGGTACCTCAGTCCCTGGGGGTTATTGGTGGCCGACCCAACAGTGCTCATTACTTCATAGGATATGTAGGGGA
TGAGCTGATCTACCTGGACCCTCACACTACACAGCTTGCGGTGGAGCCCAGTGACTGCTGTTTTGTAGAAGACGAAAGTTTCCACTGTCAGCACCCTCCA
TGTCGAATGCATGTATCCGAGATTGACCCTTCTATCGCTGTGGGCTTTTTCTGTAGATCTCAAGAAGACTTTGAGGATTGGTGCCAACAAATTAAAAAGC
TGTCTCTTTCTGGAGGAGCCCTGCCGATGTTTGAAGTGGTAGATCAGTTACCTTTACATTTATCCAATCCAGATGTGCTAAACCTCACGCCAGATTCATC
AGATGCTGACAGACTTGACAGGTTCTTTGATTCTGAAGATGAAGAATTTGAAATTTTATCACTATGAAAAGTCTGAAGAATAAAATTACCTATGGATTAG
GAACACATTATATTCTACAGCCAGGCAATAGGAGAGCAGTCACCACTGTGGGCTACAGACTGCTGGATTACTTACACAGTTCCCTGCTCCTTGGGTTTGA
TGATGAGTTTTCCAGTTACAGATACTTCCTGCTGCATTGGGGAAATGCTGCCTATATTTTTGGTGTGGCCCCTTCAGCTGTCACTCCTGGATCTAGCTGG
GATGTCAAATAACTTCATGCTTATGTTCTGTTACATTCCATAGACAGGAAAACACCACTGCTTACATCAAGAATTAGTGACAAGGACAGAAAAAGATACG
TCAGGGCTCAGCATTAGGGTATGGCATGAGAACTGGGTAGAAAGGATAGGTTTTCTTAAGTAAGTGTGCAGGAATTTTGCACTCAGAATAAGGGAAAACC
CAGGATGGCCTATCTATTGGTCTCTACTTGCATAAAGCAGGCATAGGAAGCTCCTGAGACATCAGTGGGTAGTTATTGTAAAGGGCATATTGTAAAGATG
GGCCAACAAGCAGTGGTGTTGATCTGCTGTTCCTAAAACCATGCTGGACGTGTGCAAGTTTATGTAGGCAGACTGGAATTGTACACATTGTAGACAAATG
CTTTCACATTTTTTAAGTGCAAGTCCCCCAAATACATTAAAGACAGTATGCATTTGTTTTGTTTCCTCTTTTGAAAGCAGCACTATGAGCTGAGGATGGA
TTCCAAAAGTCATGTATATTTACAGATAAACATGAGAAGTGGTCAGCTTCATAATGCTGTGATTTTATTTAAGCTTGACTTTTAAAAATGATCTCTGAAC
TACTTCAATAAAAGCAAGTCATCAAGTAAAAAAAAAAAAAAAA
5' EST: CX875591 [+]tgaaaggaatgatggacgcagctacactcacttatgataccctgcgctttgccgatactcccgactttcctgaaacagcggagccagtttgggtgctggg
aaggaaatacagtgcattgacagagaaagagcagctcttgaatgacatcacttccaggctctggtttacctacagaaggaactttccagcaatagggggc
acagggcccacgtctgacacgggctggggctgtatgctacgatgtggccagatgatctttgctcaggctttgctctgtagacacataggaagagactgga
gatgggacaaacagaaatcccaaggagaatatctgaacatccttacagcttttctggacaaaaaagatagttattattccatccaccaaattgcacaaat
gggagttggagaaggcaaatgcattggccagtggtatggtccaaacacagtggcgcaggtcctgaggaaacttgcggtttttgaccagtggagctctatt
gctgttcatatagctatggataacacagttgtgatggatgagatacgcagattatgcagagctggaaccaatgagagctccgaggctggggccctttgta
atggctatacaggcgtctctgaccccagctgctccctatggaagcctcttgtgctactcatacctcttcggctgggactaagtgatatcaatgaagccta
tattgaaacactaaagcactgctttatggtacctcagtccctgggggttattggtggccgaccccacagtgctcattacttcataggatatgtaggggat
gagctgatctacctggaccctcacactacacag
3' EST: CX875590 [+]gatgacttgcttttattgaagtagttcagagatcatttttaaaagtcaagcttaaataaaatcacagcattatgaagctgaccacttctcatgtttatct
gtaaatatacatgacttttggaatccatcctcagctcatagtgctgctttcaaaagaggaaacaaaacaaatgcatactgtctttaatgtatttggggga
cttgcacttaaaaaatgtgaaagcatttgtctacaatgtgtacaattccagtctgcctacataaacttgcacacgtccagcatggttttaggaacagcag
atcaacaccactgcttgttggcccatctttacaatatgccctttacaataactacccactgatgtctcaggagcttcctatgcctgctttatgcaagtag
agaccaatagataggccatcctgggttttcccttattctgagtgcaaaattcctgcacacttacttaagaaaacctatcctttctacccagttctcatgc
cataccctaatgctgagccctgacgtatctttttctgtccttgtcactaattcttgatgtaagcagtggtgttttcctgtctatggaatgtaacagaaca
taagcatgaagttatttgacatcccagctagatccaggagtgacagctgaaggggccacaccaaaaatataggcagcatttccccaatgcagcaggaagt
atctgtaactggaaaactcatcatcaaacccaaggagcagggaactgtgtaagtaatccagcagtctgtagcccacagtggtgactgctctcctattgcc
tggctgt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: